Comparative transcriptome profiling of genes and pathways related to resistance against powdery mildew in two contrasting melon genotypes

2018 ◽  
Vol 227 ◽  
pp. 169-180 ◽  
Author(s):  
Qianglong Zhu ◽  
Peng Gao ◽  
Yan Wan ◽  
Haonan Cui ◽  
Chao Fan ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Transcriptome Profiling ◽  
Comparative Transcriptome

Comparative transcriptome profiling of Chinese wild grapes provides insights into powdery mildew resistance

2021 ◽  
Author(s):  
Chen Jiao ◽  
Xuepeng Sun ◽  
Xiaoxiao Yan ◽  
Xiaozhao Xu ◽  
Qin Yan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Powdery Mildew ◽  
Fungal Pathogen ◽  
Fatty Acid Biosynthesis ◽  
Transcriptome Profiling ◽  
Wine Industry ◽  
Post Inoculation ◽  
Comparative Transcriptome ◽  
Genes Encoding ◽  
Stage Analysis

Erysiphe necator, the fungal pathogen of grape powdery mildew disease, poses a great threat to the grape fruit market and wine industry. To better understand the molecular basis of grape responses to E. necator, we performed comparative transcriptome profiling on two Chinese wild grape accessions with varying degrees of resistance to E. necator. A total of 2,856, 2,678 and 1,542 differentially expressed genes (DEGs) were identified in the susceptible Vitis pseudoreticulata ‘Hunan-1’ at 6, 24, and 96 hours post-inoculation of E. necator, respectively, while 1,921, 2,498 and 3,249 DEGs were identified in the resistant V. quinquangularis ‘Shang-24’. ‘Hunan-1’ had substantially larger fraction of down-regulated genes than ‘Shang-24’ at every infection stage. Analysis of DEGs revealed that up-regulated genes were mostly associated with defense response and disease resistance-related metabolite biosynthesis, while signaling genes were significantly suppressed in ‘Hunan-1’. Interestingly, fatty acid biosynthesis and elongation related genes were suppressed by the fungus in ‘Shang-24’ but somehow induced in ‘Hunan-1’, consistent with that E. necator is likely a fatty acid auxotroph that requires lipids from the host. Moreover, genes involved in biosynthesis and signaling of phytohormones, such as jasmonic acid and cytokinin, as well as genes encoding protein kinases and NLR proteins were differentially responded to E. necator in the two wild grapes. The variation of gene regulation associated with nutrient uptake by the fungus and with signaling transduction and pathogen recognition suggests a multi-layered regulatory network that works in concert to defend fungal pathogen infections.

scholarly journals Characterization of novel regulators for heat stress tolerance in tomato from Indian sub-continent

2019 ◽  
Author(s):  
Sonia Balyan ◽  
Sombir Rao ◽  
Sarita Jha ◽  
Chandni Bansal ◽  
Jaishri Rubina Das ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Heat Stress ◽  
Functional Characterization ◽  
Transcriptome Profiling ◽  
Negative Regulator ◽  
Comparative Transcriptome ◽  
Regulatory Pathways ◽  
Cool Region ◽  
Promoter Swapping

AbstractThe footprint of tomato cultivation, a cool region crop that exhibits heat stress (HS) sensitivity, is increasing in the tropics/sub-tropics. Knowledge of novel regulatory hot-spots from varieties growing in the Indian sub-continent climatic zones could be vital for developing HS-resilient crops. Comparative transcriptome-wide signatures of a tolerant (CLN1621L) and sensitive (CA4) cultivar-pair short-listed from a pool of varieties exhibiting variable thermo-sensitivity using physiological, survival and yield-related traits revealed redundant to cultivar-specific HS-regulation with more up-regulated genes for CLN1621L than CA4. The anatgonisiticly-expressing genes include enzymes; have roles in plant defense and response to different abiotic stresses. Functional characterization of three antagonistic genes by overexpression and TRV-VIGS silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis), that are up-regulated in tolerant cultivar, as positive regulators of HS-tolerance and Solyc03g020030 (Pin-II proteinase inhibitor), that is down-regulated in CLN1621L, as negative regulator of thermotolerance. Transcriptional assessment of promoters of these genes by SNPs in stress-responsive cis-elements and promoter swapping experiments in opposite cultivar background showed inherent cultivar-specific orchestration of transcription factors in regulating transcription. Moreover, overexpression of three ethylene response transcription factors (ERF.C1/F4/F5) also improved HS-tolerance in tomato. This study identifies several novel HS-tolerance genes and provides proof of their utility in tomato-thermotolerance.HighlightNovel heat stress regulatory pathways uncovered by comparative transcriptome profiling between contrasting tomato cultivars from Indian sub-continent for improving thermotolerance. (20/30)

Comparative transcriptome profiling of developing caryopses from two rice cultivars with differential dormancy

2013 ◽  
Vol 170 (12) ◽  
pp. 1090-1100 ◽  
Author(s):  
Sun Mi Huh ◽  
Yong-sic Hwang ◽  
Young Seop Shin ◽  
Myung Hee Nam ◽  
Dool Yi Kim ◽  
...  
Keyword(s):  
Transcriptome Profiling ◽  
Rice Cultivars ◽  
Comparative Transcriptome
Sign in / Sign up

Export Citation Format

Share Document