Nitric oxide is involved in ethylene-induced adventitious root development in cucumber (Cucumis sativus L.) explants

2017 ◽  
Vol 215 ◽  
pp. 65-71 ◽  
Author(s):  
Xiao-Ting Xu ◽  
Xin Jin ◽  
Wei-Biao Liao ◽  
Mohammed Mujitaba Dawuda ◽  
Xue-Ping Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cucumis Sativus ◽  
Root Development ◽  
Adventitious Root ◽  
Cucumis Sativus L ◽  
Adventitious Root Development

scholarly journals Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yutong Li ◽  
Yue Wu ◽  
Weibiao Liao ◽  
Linli Hu ◽  
Mohammed Mujitaba Dawuda ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Root Development ◽  
Adventitious Root ◽  
Adventitious Root Development

scholarly journals Proteomic analysis reveals key proteins involved in ethylene-induced adventitious root development in cucumber (Cucumis sativus L.)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10887
Author(s):  
Jian Lyu ◽  
Yue Wu ◽  
Xin Jin ◽  
Zhongqi Tang ◽  
Weibiao Liao ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Cucumis Sativus ◽  
Root Development ◽  
Proteomic Analysis ◽  
Adventitious Root ◽  
Adventitious Rooting ◽  
Ethylene Synthesis ◽  
Adventitious Root Development ◽  
Endogenous Ethylene ◽  
Exogenous Ethylene

The mechanisms involved in adventitious root formation reflect the adaptability of plants to the environment. Moreover, the rooting process is regulated by endogenous hormone signals. Ethylene, a signaling hormone molecule, has been shown to play an essential role in the process of root development. In the present study, in order to explore the relationship between the ethylene-induced adventitious rooting process and photosynthesis and energy metabolism, the iTRAQ technique and proteomic analysis were employed to ascertain the expression of different proteins that occur during adventitious rooting in cucumber (Cucumis sativus L.) seedlings. Out of the 5,014 differentially expressed proteins (DEPs), there were 115 identified DEPs, among which 24 were considered related to adventitious root development. Most of the identified proteins were related to carbon and energy metabolism, photosynthesis, transcription, translation and amino acid metabolism. Subsequently, we focused on S-adenosylmethionine synthase (SAMS) and ATP synthase subunit a (AtpA). Our findings suggest that the key enzyme, SAMS, upstream of ethylene synthesis, is directly involved in adventitious root development in cucumber. Meanwhile, AtpA may be positively correlated with photosynthetic capacity during adventitious root development. Moreover, endogenous ethylene synthesis, photosynthesis, carbon assimilation capacity, and energy material metabolism were enhanced by exogenous ethylene application during adventitious rooting. In conclusion, endogenous ethylene synthesis can be improved by exogenous ethylene additions to stimulate the induction and formation of adventitious roots. Moreover, photosynthesis and starch degradation were enhanced by ethylene treatment to provide more energy and carbon sources for the rooting process.

scholarly journals Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber (Cucumis sativus L.) explants

2019 ◽  
Author(s):  
Li Yutong ◽  
Yue Wu ◽  
Weibiao Liao ◽  
Linli Hu ◽  
Mohammed Mujitaba Dawuda ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cucumis Sativus ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Adventitious Rooting ◽  
Adventitious Root Formation ◽  
No Donor ◽  
Cucumis Sativus L ◽  
Positive Effects ◽  
Relative Expression Level

Abstract Background: Brassinolide (BR), as a new type of plant hormones, is involved in the process of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant developmental processes and also response tolerance to abiotic stresses in plants. In my study, the main purpose is to explore whether nitric oxide (NO) is involved in the process of BR-induced adventitious root formation in cucumber (Cucumis sativus L.), and whether it plays a certain role. Results: Exogenous application of 1 μM BR significantly promoted adventitious rooting, while high concentrations of BR (2-8 μM) effectively inhibited adventitious rooting. NO donor (S-nitroso-N-acerylpenicillamine, SNAP) promoted the occurrence of adventitious roots. Simultaneously, BR and SNAP mixed treatment significantly promoted adventitious rooting and the promoted effects was significantly superior to the application of BR or SNAP alone. Moreover, NO scavenger (c-PTIO) and inhibitors (L-NAME and Tungstate) inhibited the positive effects of BR on adventitious rooting. BR at 1 μM also increased endogenous NO content, NO synthase (NOS-like) and Nitrate reductase (NR) activities, while BRz (a specific BR biosynthesis inhibitor) decreased these effects. In addition, the relative expression level of NR was up-regulated by BR and SNAP, whereas BRz down-regulated it. The application of NO inhibitor (Tungstate) in BR also inhibited the up-regulation of NR. Conclusion: BR promoted the formation of adventitious roots via inducing the generation of endogenous NO.

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