Novel 307 polymorphic SSR markers from BAC-end sequences in walnut ( Juglans regia L.): Effects of motif types and repeat lengths on polymorphism and genetic diversity

2016 ◽  
Vol 213 ◽  
pp. 1-4 ◽  
Author(s):  
Adi Surya Ikhsan ◽  
Hayat Topçu ◽  
Mehmet Sütyemez ◽  
Salih Kafkas
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Juglans Regia ◽  
End Sequences

scholarly journals Genetic diversity detection of seed-propagated walnut (Juglans regia L.) germplasm from Eastern Anatolia using SSR markers

2020 ◽  
Vol 32 (1) ◽  
pp. 37-46
Author(s):  
Emine Orhan ◽  
Sadiye Peral Eyduran ◽  
Danijela Poljuha ◽  
Meleksen Akin ◽  
Tim Weber ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Ssr Markers ◽  
Phenotypic Variability ◽  
Juglans Regia ◽  
Molecular Data ◽  
Fruit Weight ◽  
Morphological And Molecular Data ◽  
Bud Breaking ◽  
Seed Propagation

AbstractContinuous seed propagation in Turkey has given rise to a great number of seedling walnut trees which represents valuable walnut genetic resources. The number of native walnut trees is estimated to be over 5 million in Turkey and they possess large phenotypic variability in yield, nut and kernel characteristics, late bud breaking, late flowering, winter hardiness and tolerance to diseases. Progress in walnut breeding requires the exploitation of genetic variation among cultivars and landraces. In this study, we used 32 local diverse walnut genotypes obtained from seeds and 2 standard cultivars (‘Sebin’ and ‘Bilecik’). This study implemented 21 previously used simple sequence repeats (SSR) markers to determine genetic diversity. The analysis revealed 135 alleles with an average of 6.43 alleles per locus. Genetic similarity ranged from 0.23 (for samples KW22 and KW29) to 0.87 (for samples KW27 and KW28). The highest number of alleles per locus was obtained from WGA276 locus (11 alleles), followed by WGA054 (9 alleles), WGA202 and WGA321 (8 alleles) while the lowest number was detected in WGA027. According to the morphological and molecular data, the genotypes differed from each other and the cvs. Sebin and Bilecik. The majority of the genotypes had higher fruit weight and some of the genotypes had higher kernel ratio than cvs. Sebin and Bilecik implying the importance of registering genotypes as cultivars. This research provides information on the genetic relationship of walnut genotypes and cultivars and emphasises the importance of protection and utilisation of seed-propagated walnut genetic resources.

scholarly journals Genetic diversity of walnut (Juglans regia L.) seedlings through SSR markers in north-western Himalayan region of Jammu

2020 ◽  
Vol 49 (4) ◽  
pp. 1003-1012
Author(s):  
Rafiq Ahmad Shah ◽  
Parshant Baksi ◽  
Amit Jasrotia ◽  
Deep JI Bhat ◽  
Rucku Gupta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Decision Making ◽  
Ssr Markers ◽  
Juglans Regia ◽  
Germplasm Management ◽  
Expected Heterozygosity ◽  
Western Himalayan Region ◽  
Ssr Primers ◽  
Genetic Dissimilarity ◽  
North Western

screening of 25 SSR markers, revealed 23 clear and consistent amplification profiles in the entire walnut germplasm set. A total of 54 alleles were amplified by SSR primers and the number of alleles range from 2 to 3. The PIC value ranged from 0.36 to 0.68. The dendrogram classified all genotypes into two main clusters with various degrees of subclustering. Estimated genetic dissimilarity coefficient ranged from 0.36 to 0.85. Through model-based cluster analysis all genotypes were grouped into 5 genetically distinct subpopulations. The expected heterozygosity at a given locus was found to range from 0.520 to 0.5477. Similarly, population differentiation measurements (Fst) ranged from 0.2286 to 0.2909. These findings would be helpful for decision making in future walnut breeding studies, germplasm management activities to maximize genetic diversity in walnut germplasm and may also prove useful in future for conducting association mapping in walnut for different traits.

scholarly journals Analysis of genetic diversity and structure in a worldwide walnut (Juglans regia L.) germplasm using SSR markers

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0208021 ◽  
Author(s):  
Anthony Bernard ◽  
Teresa Barreneche ◽  
Fabrice Lheureux ◽  
Elisabeth Dirlewanger
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Juglans Regia ◽  
Genetic Diversity And Structure

scholarly journals Population Structure, Genetic Diversity, and Gene Introgression of Two Closely Related Walnuts (Juglans regia and J. sigillata) in Southwestern China Revealed by EST-SSR Markers

Forests ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 646 ◽  
Author(s):  
Xiao-Ying Yuan ◽  
Yi-Wei Sun ◽  
Xu-Rong Bai ◽  
Meng Dang ◽  
Xiao-Jia Feng ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Juglans Regia ◽  
Southwestern China ◽  
Gene Introgression ◽  
High Genetic Diversity ◽  
Important Species ◽  
Sympatric Distribution ◽  
Geographical Regions ◽  
Genetic Clusters

The common walnut (Juglans regia L.) and iron walnut (J. sigillata Dode) are well-known economically important species cultivated for their edible nuts, high-quality wood, and medicinal properties and display a sympatric distribution in southwestern China. However, detailed research on the genetic diversity and introgression of these two closely related walnut species, especially in southwestern China, are lacking. In this study, we analyzed a total of 506 individuals from 28 populations of J. regia and J. sigillata using 25 EST-SSR markers to determine if their gene introgression was related to sympatric distribution. In addition, we compared the genetic diversity estimates between them. Our results indicated that all J. regia populations possess slightly higher genetic diversity than J. sigillata populations. The Geostatistical IDW technique (HO, PPL, NA and PrA) revealed that northern Yunnan and Guizhou provinces had high genetic diversity for J. regia while the northwestern Yunnan province had high genetic diversity for J. sigillata. AMOVA analysis revealed that significant genetic variation was mainly distributed within population as 73% in J. regia and 76% in J. sigillata. The genetic differentiation (FST) was 0.307 between the two walnut species (p < 0.0001), which was higher than FST values within populations (J. regia FST = 0.265 and J. sigillata FST = 0.236). However, the STRUCTURE analysis of the J. regia and J. sigillata populations revealed two genetic clusters in which gene introgression exists, therefore, the boundary of separation between these two walnut species is not clear. Moreover, these results were validated by NJ and UPGMA analysis with additional conformation from the PCoA. Based on the SSR data, our results indicate that J. sigillata is an ecotype of J. regia. Taken together, these results reveal novel information on population genetics and provide specific geographical regions containing high genetic diversity of the Juglans species sampled, which will assist in future conservation management.

Genetic diversity and population structure of Populus yunnanensis revealed by SSR markers

2020 ◽  
Vol 52 (6) ◽  
Author(s):  
Anpei Zhou ◽  
Dan Zong ◽  
Peihua Gan ◽  
Yao Zhang ◽  
Dan Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Ssr Markers

Genetic diversity in Chinese rapeseed (Brassica napus) cultivars based on EST-SSR markers

2009 ◽  
Vol 17 (5) ◽  
pp. 482 ◽  
Author(s):  
Dai Li-chuan ◽  
Zhang Ming-long ◽  
Liu Ji-ye ◽  
Li Xiao-bai ◽  
Cui Hai-rui
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Ssr Markers

Genetic diversity in adzuki bean and its relatives based on SSR markers

2011 ◽  
Vol 19 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Wang Lixia ◽  
Cheng Xuzhen ◽  
Wang Suhua
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Adzuki Bean
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