Validation of molecular markers associated with fruit ripening day of Japanese pear (Pyrus pyrifolia Nakai) using variance components

2016 ◽  
Vol 199 ◽  
pp. 9-14 ◽  
Author(s):  
Sogo Nishio ◽  
Takeshi Hayashi ◽  
Toshiya Yamamoto ◽  
Masahiko Yamada ◽  
Norio Takada ◽  
...  
HortScience ◽  
2011 ◽  
Vol 46 (11) ◽  
pp. 1540-1544 ◽  
Author(s):  
Sogo Nishio ◽  
Masahiko Yamada ◽  
Yutaka Sawamura ◽  
Norio Takada ◽  
Toshihiro Saito

The effectiveness of detected quantitative trait loci (QTLs) and molecular markers associated with them in tree fruit breeding is measured by the percentages of the variance associated with detected QTL effects accounting for not phenotypic variance, but genetic variance of the trait. The genetic variance can be obtained by subtracting environmental variance from the phenotypic variance. Once accurate environmental variance components are obtained for a given selection field, environmental variances under any number of replications and measurement repetitions can be estimated. We estimated environmental variance components of fruit ripening date measured by days in a Japanese pear (Pyrus pyrifolia Nakai) breeding field in the National Institute of Fruit Tree Science, Tsukuba, Ibaraki, Japan. We estimated variance among fruits within a tree (σf2) as 25.6, among trees within a genotype (σt2) as 0.2, among years (σy2) as 9.4, associated with genotype × year interaction (σgy2) as 7.9, and associated with tree × year interaction (σty2) as 1.2. Because σf2 was the largest environmental variance component, increasing the number of fruit evaluated would most effectively reduce the environmental variance, and tree replication would not because of very small σt2 and σty2. The 95% confidence limit of a genotypic value was ± 10 days in the evaluation of five fruits on a single tree in a year and ± 7 days over 2 years. Broad-sense heritability in a family, each offspring in which was evaluated using five fruits on a single tree in a single year, was estimated at 0.83 for three full-sib families analyzed.


2002 ◽  
Vol 19 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Carlos CASTILLO ◽  
Takeshi TAKASAKI ◽  
Toshihiro SAITO ◽  
Shigemi NORIOKA ◽  
Tetsu NAKANISHI

2005 ◽  
Vol 74 (3) ◽  
pp. 255-257 ◽  
Author(s):  
Haruyuki Kuroda ◽  
Toshihiko Sugiura ◽  
Hiroyoshi Sugiura

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