Culture vessel and auxin treatments affect in vitro rooting and ex vitro survival of six Arachis paraguariensis genotypes

2015 ◽  
Vol 183 ◽  
pp. 167-171 ◽  
Author(s):  
O.O. Aina ◽  
K.H. Quesenberry ◽  
M. Gallo
Keyword(s):  
In Vitro Rooting ◽  
Culture Vessel ◽  
Ex Vitro ◽  
Arachis Paraguariensis

scholarly journals Micropropagation of Achillea millefolium L. on half-strength ms medium and direct rooting and acclimatization of microshoots in hydroponic culture

2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Nutrient Solution ◽  
Ph Value ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Efficient Production ◽  
Ms Medium ◽  
Ex Vitro ◽  
Half Strength

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.

scholarly journals A COMPREHENSIVE DESCRIPTION OF EX-VITRO ROOTING OF PISTACHIO ROOTSTOCKS GROWN IN PHOTOMIXOTROPHIC AND PHOTOAUTOTROPHIC CONDITION

2020 ◽  
Vol 36 ◽  
pp. 66-74
Author(s):  
Mohammad Javad Mahmoudi Meimand ◽  
Mohammad Hossein Shamshiri ◽  
Khalil Malekzadeh ◽  
Mohammad Reza Dehghani
Keyword(s):  
Sucrose Concentration ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Naphthalene Acetic Acid ◽  
Ex Vitro ◽  
Indole Butyric Acid ◽  
Micro Propagation ◽  
The Media ◽  
Photoautotrophic Condition

As a basic principle, ex-vitro rhizogenesis increases the micropropagation efficiency of Micro propagation in any plant from both biological and economic viewpoints. In the current study, we surveyed the effects of number of air exchanges along with sucrose concentration on in-vitro rooting of two pistachio rootstocks consisting of UCB1 and Qazvini versus ex-vitro rooting. Based on our findings for the UCB1 rootstock, microshoot ex-vitro rooting reached the highest percentage (63.70 %) after six weeks' treatment with indole butyric acid (IBA) (5000 ppm) and free naphthalene acetic acid (NAA), while for Qazvini rootstock treated with NAA (6000 ppm) along with IBA (5000 ppm), rooting achieved 35.06%. Photomixotrophic resulted from decreasing sucrose concentration from 30 to 15 (g L-1) in corporation with ventilation condition increased UCB1 rooting (67.89%) as well as plant survival (58.34%). For Qazvini rootstock, maximum sucrose concentration (30 g L-1) improved rooting parameters. For in-vitro rooting experiment, rooting percentage of UCB1 plantlets as well as the main and lateral produced roots were higher in media supplemented with (1 mg l-1) IBA, free NAA, and BA. Regarding Qazvini rootstock, the highest in-vitro rooting percentage (43.75%) and root length were associated with the media supplemented with BA (0.5 mg l-1), IBA (2 mg l-1), and NAA (2 mg l-1). As a result, for both the studied rootstocks, better rooting parameters were observed in the ex-vitro rooted microshoots than in-vitro rooted.

In vitro Rooting and Ex vitro Plantlet Establishment of BARI Banana 1 (Musa sp.) as Influenced by Different Concentration of IBA (Indole 3-butyric Acid)

2004 ◽  
Vol 3 (2) ◽  
pp. 196-199 ◽  
Author(s):  
M.M.H. Molla ◽  
M. Dilafroza Khanam . ◽  
M.M. Khatun . ◽  
M. Al-Amin . ◽  
M.A. Malek .
Keyword(s):  
Butyric Acid ◽  
In Vitro Rooting ◽  
Ex Vitro ◽  
Musa Sp

scholarly journals Anatomical and physiological modifications of micropropagated 'Caipira' banana plants under natural lighy

2009 ◽  
Vol 66 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Frederico Henrique da Silva Costa ◽  
Moacir Pasqual ◽  
Jonny Everson Scherwinski Pereira ◽  
Evaristo Mauro de Castro
Keyword(s):  
Sucrose Concentration ◽  
In Vitro Rooting ◽  
Production Costs ◽  
Tropical Species ◽  
Natural Light ◽  
Artificial Light ◽  
Ex Vitro ◽  
Cultivation Conditions ◽  
Growth Environment

Research about the use of natural light associated to changes in sucrose levels demonstrated potential in promoting in vitro hardiness of tropical climate species, as well as reducing production costs. However, little is known about physiological and structural changes that happen in the process. This study evaluated the physiological and anatomic performance, and ex vitro survival of micropropagated banana plants in response to cultivation conditions, in the stage of in vitro rooting. Shoots of the 'Caipira' cultivar were cultivated in MS medium, supplemented with 1 mg L-1 NAA and 6 g L-1 agar, in which the following treatments were applied: two sucrose concentrations (15 g L-1 or 30 g L-1) and two cultivation conditions (Natural light - greenhouse and Artificial light - growth chamber). At the end of 45 days, the contents of chlorophyll a, b and total, the relative water content in the tissues, anatomic characteristics and the ex vitro survival were evaluated. Effects of growth environment and sucrose concentration were observed on micropropagated 'Caipira' banana anatomy, physiology and survival. In vitro rooting of the shoots under natural light in the medium containing 15 g L-1 or 30 g L-1 sucrose promoted major alteration in the increase of palisade and spongy parenchyma, as well as reducing leaf water loss and plant death. The results obtained in the present study confirm the potential of the use of natural light as a substitute for artificial light for micropropagation of tropical species.

scholarly journals In vitro rooting and anatomical study of leaves and roots of in vitro and ex vitro plants of Prunus x davidopersica 'Piroska'

2001 ◽  
Vol 7 (1) ◽  
Author(s):  
E. Jámbor-Benczúr ◽  
J. Kissimon ◽  
M. Fábián ◽  
A. Mészáros ◽  
Z. Sinkó ◽  
...  
Keyword(s):  
Vascular System ◽  
Anatomical Study ◽  
In Vitro Rooting ◽  
Ex Vitro ◽  
Air Space ◽  
Xylem Elements ◽  
Anatomical Characters ◽  
Leaves And Roots ◽  
Proliferation Period

The process of in vitro rooting and the anatomical characters of in vitro and ex vitro leaves and roots of Prunus x davidopersica 'Piroska' were studied. Best rooting percentage (50%) and highest root number (5.0) was achieved in spring on a medium containing 0.1 mg/I NAA + 30 g/1 glucose. At the end of rooting the parenchyma of the in vitro leaves was more loose and spongy, than during the proliferation period. In the first newly developed leaf of an acclimatised plant, the parenchyma was much more developed, contained less row of cells and less air space too, compared to the leaves developed in the field. The in vitro developed root had a broad cortex and narrow vascular cylinder with less developed xylem elements, but at the end of the acclimatisation the vascular system became dominant in the root.

scholarly journals Improvement of Ex Vitro Desiccation through Elevation of CO2 Concentration in the Atmosphere of Culture Vessels during In Vitro Growth

HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 1006-1012 ◽  
Author(s):  
Kourosh Vahdati ◽  
Zeinab Maleki Asayesh ◽  
Sasan Aliniaeifard ◽  
Charles Leslie
Keyword(s):  
Water Loss ◽  
Compatible Solutes ◽  
Co2 Concentration ◽  
Dynamic Responses ◽  
Stomatal Aperture ◽  
Culture Vessel ◽  
Ex Vitro ◽  
Negative Effects ◽  
Key Steps

Acclimatization of in vitro plantlets is one of the key steps in successful tissue culture propagation. Gaseous atmosphere during in vitro culture can influence the rate of ex vitro acclimation of the plantlets produced. In the current study, effects of elevated CO2 concentration on the leaf water loss dynamic responses of in vitro–produced walnut leaves during ex vitro desiccation were investigated. Elevated CO2 concentration in the headspace of culture vessels caused a considerable decrease in stomatal aperture. Although the traits related to stomatal size were not influenced by CO2 elevation, the number of small stomata was increased, and the number of large stomata was decreased at elevated CO2 concentration. Higher CO2 concentration resulted in a lower transpiration rate and a higher relative water content (RWC) during ex vitro desiccation. This improvement was due to decreased stomatal aperture during the first phase of water loss. Osmotic potential (ψs) was decreased under an elevated CO2 concentration, but no influence was observed on the concentration of compatible solutes. In conclusion, increasing the CO2 concentration of culture vessel headspace can be an efficient tool for improving acclimation of in vitro–grown walnuts without negative effects on plantlet growth.

scholarly journals Simultaneous direct ex vitro rooting and adaptation of the blue honeysuckle micro-sprouts (Lonicera Caerulea L. var. Kamtschatica)

2020 ◽  
Vol 58 (3) ◽  
pp. 298-310
Author(s):  
E. V. Kolbanova
Keyword(s):  
Surface Layer ◽  
Thermal Treatment ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Ex Vitro ◽  
One Stage ◽  
Planting Material ◽  
Peat Surface ◽  
Blue Honeysuckle

Direct rooting of regenerative plants under ex vitro conditions is of great importance in agricultural biotechnology, as it leads to acceleration of micropropagation process by eliminating the stage of in vitro rooting and reduces the costs for obtaining healthy planting material of fruit and small-fruit crops. Possibility of direct ex vitro rooting and adaptation of blue honeysuckle microshoots at one stage with no stage of in vitro rooting has been determined. For ex vitro rooting and adaptation of honeysuckle microshoots, it is required to use non-sterile substrate: Sphagnum L. moss with a surface layer of peat (0.5 cm). The rate of rooted microshoots on this substrate makes 72-84 % during summer-autumn period and not less than 60 % during winter period. Thermal treatment of the peat surface layer and the use of aqueous IBA solutions at ex vitro rooting stage will be economically justified during propagation of individual varieties characterized by low proliferation activity in in vitro culture or low ex vitro rhizogenic activity, as well as breeding novelties requiring rapid propagation and obtaining of large amount of planting material. Thermal treatment of the peat surface layer allows increasing the rate of rooted microshoots of Volkhov variety by 10.7 %, Krupnoplodnaya variety by 13.2 %, Pavlovskaya variety by 3.8 % when rooting during spring period. Use of aqueous solutions of IBA increases the rate of rooted plants by 6.2-6.7 % in Vostorg and Krupnoplodnaya varieties when rooting during summer period. Carrying out of one cutting of ex vitro rooted microshoots and further cuttings of obtained material can be used to increase the yield of planting material of blue honeysuckle varieties. Ex vitro rooting and adaptation of blue honeysuckle microshoots at one stage with further ex vitro cutting increases production profitability by 3.5 and 12.9 times in comparison with conventional method of clonal micropropagation of blue honeysuckle.

scholarly journals IN VITRO PROPAGATION OF PATUMMA (CURCUMA ALISMATIFOLIA GAGNEP.)

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 585d-585
Author(s):  
Surawit Wannakrairoj
Keyword(s):  
Survival Rate ◽  
In Vitro Propagation ◽  
Culture Vessel ◽  
Multiplication Rate ◽  
Ex Vitro ◽  
Lateral Bud ◽  
The Media ◽  
Pre Treatment ◽  
Better Than

Patumma, a native of Thailand, is a new ornamental crop. Its increased demand for export make the species vulnerable to extinction. Cloning methodology is thus needed for the production of both existing and newly developed clone. Young inflorescent segment and lateral bud from rhizome can be used as explants. For decontamination of the lateral bud, the success was depended both on pre-treatment and disintestation procedure. The bud from dried rhizome was better than one from fresh rhizome. Prior to disinfestation with sodium hypochlorite, pre-treatment of rhizome in 52°C water for 5 minutes could replaced the use of antibiotic. Plantlet were placed on modified MS media with 0, 6.67, 13.32, 19.98 and 26.64 μmol/l benzyladenine (BA) or 0.19, 0.56, 1.67 and 5 μmol/l kinetin. The best multiplication rate of 4.83 fold was obtained when longitudinally-divided rhizome was on the medium with 13.32 μmol/l BA. The result also showed that wild-collected and selected clones responded to the media similarly. When the MS media modified with 13.32, 15.54, 17.76 and 19.98 μmol/l BA in combination with 15, 30 and 45 g/l sucrose were tested, the multiplication rate of non-divided plantlets were all the same. Acclimatization by open the culture vessel for 3 day improved plantlet survival rate ex vitro.

scholarly journals Optimization of IBA for Efficient Survival of Micropropagated Garcinia indica Choisy Shoots Using in-vitro and ex-vitro Rooting Techniques

2019 ◽  
pp. 1-9
Author(s):  
Gauri Mulik ◽  
Shubha Manvi ◽  
Gauri Ingale ◽  
Varsha A. Parasharami
Keyword(s):  
Tissue Culture ◽  
Farmyard Manure ◽  
Ex Vitro Rooting ◽  
In Vitro Rooting ◽  
Root Induction ◽  
Ex Vitro ◽  
Interesting Phenomenon ◽  
National Chemical Laboratory ◽  
Garcinia Indica

Aims: The study focuses on optimization of concentration and time of rooting hormone exposure of IBA (Indole-3-Butyric Acid) for efficient survival of tissue culture raised Garcinia indica Choisy plantlets for in-vitro and ex-vitro rooting techniques. Study Design: The subcultured microshoots of Garcinia indica were subjected to in-vitro and ex-vitro rooting trials by treating them with IBA of varying concentration and time, to standardize these particular parameters required by this auxin to induce rooting. Place and Duration of Study: Rooting trials were carried out in Plant tissue culture-Biochemical Sciences Division of CSIR- National Chemical Laboratory, Pune 411008 between June 2018 and April 2019. Methodology: Regularly subcultured five to six years old shoots from female trees of Garcinia indica were used for the study. Various concentrations of IBA in correlation with time were used for in-vitro and ex-vitro root induction. The rooted plantlets were then transferred to polyhouse for acclimatization and will further be planted in open field locations in June 2019. Results: Induction of rooting was observed within thirty days of treatment with IBA. It was observed that 500ppm of IBA gave 30% rooting for in-vitro rooting trials whereas 2000ppm of IBA induced 80% rooting for shoots given ex-vitro rooting treatments. An interesting phenomenon that was observed for 70% of the shoots which failed to induce rooting by in-vitro treatment was that they survived with 100% rooting success under ex-vitro rooting conditions. The hardened plantlets were successfully acclimatized in the polyhouse with survival rate of 90% and were further transferred to polythene bags with rooting mixtures of sand: farmyard manure: coco peat (1:1:2). These plantlets have been healthy for the last 6 to 9 months and will be transported for field trials in June 2019. Conclusion: Ex-vitro rooting technique was found to be more effective than in-vitro rooting. Thus, by optimizing the rooting hormone parameters, female plants of Garcinia indica can be successfully grown using tissue culture technology and can be propagated in large numbers to increase the female plant number in plantations.

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