A novel in vitro germination method revealed the influence of environmental variance on the pecan pollen viability

2015 ◽  
Vol 181 ◽  
pp. 43-51 ◽  
Author(s):  
Hua-Zheng Peng ◽  
Qun-Ying Jin ◽  
Hua-Lin Ye ◽  
Tang-Jun Zhu
Keyword(s):  
Pollen Viability ◽  
In Vitro Germination ◽  
Environmental Variance

Viability and in Vitro Germination of Johnsongrass (Sorghum Halepense) Pollen

2007 ◽  
Vol 21 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Ian C. Burke ◽  
John W. Wilcut ◽  
Nina S. Allen
Keyword(s):  
Suspension Culture ◽  
Boric Acid ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Calcium Nitrate ◽  
Medium Composition ◽  
In Vitro Tests ◽  
In Vitro Germination ◽  
Agar Culture

A high proportion of viable pollen grains must germinate to study the physiology of pollen growth to reduce the confounding effects of environmental influences on pollen germination. The objectives of this study were to evaluate the nuclear state and develop a suitable medium and culture method for in vitro germination of johnsongrass pollen. Johnsongrass pollen was trinucleate, and in vitro tests for pollen viability using Alexander's stain and a fluorochromatic reaction method (FCR) indicated johnsongrass pollen was viable (92.6 to 98.4%). A factorial treatment arrangement of four concentrations of sucrose, two concentrations of boric acid, and two concentrations of calcium nitrate were used to determine the optimum pollen-germination medium composition in suspension culture, agar culture, and cellophane membrane culture. Germination was highest in a suspension culture with a medium containing 0.3 M sucrose, 2.4 mM boric acid, and 3 mM calcium nitrate. Pollen germination using this medium was 78.9% when anthers were harvested just before anthesis.

scholarly journals Technique for In Vitro Pollen Germination and Short-term Pollen Storage in Caladium

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 365-367 ◽  
Author(s):  
Zhanao Deng ◽  
Brent K. Harbaugh
Keyword(s):  
Pollen Germination ◽  
Large Scale ◽  
Pollen Viability ◽  
Storage Conditions ◽  
In Vitro Germination ◽  
Short Term ◽  
Pollen Storage ◽  
Breeding Programs ◽  
Sucrose Level

The sporadic nature of inflorescence production and flower protogyny in caladium (Caladium ×hortulanum Birdsey) makes it desirable to store pollen and to rapidly assess its viability for cross-pollinations in breeding programs. This study was conducted to develop a procedure to determine caladium pollen viability and to use that procedure to evaluate the effect of short-term storage conditions on pollen viability. The sucrose level in the culture medium was found to have a significant impact on the in vitro germination of caladium pollen; a concentration of 6.8% was determined to be optimal for pollen germination. Caladium pollen lost viability within 1 day under room (24 °C) or freezing (-20 °C) temperatures, but could be stored at 4 °C for 2 to 4 days. Pollen stored at 4 °C produced successful pollinations. Data obtained from large-scale greenhouse pollinations supported use of this in vitro germination assay as a convenient way to evaluate caladium pollen viability (and fertility).

scholarly journals Viability of pollen grains and stigma receptivity in Desert Rose

2022 ◽  
Vol 28 (1) ◽  
pp. 92-98
Author(s):  
Cristiane Gonçalves Souza ◽  
Sabrina Maihave Barbosa Ramos ◽  
Silvia Nietsche ◽  
Clivia Carolina Fiorilo Possobom ◽  
Elka Fabiana Aparecida Almeida ◽  
...  
Keyword(s):  
Abiotic Factors ◽  
Pollen Viability ◽  
Oxygen Demand ◽  
Pollen Grains ◽  
Floral Biology ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Stigma Receptivity ◽  
Flower Opening

Abstract Adenium obesum (Forssk.) Roem. & Schult., popularly known as desert rose, has become a valuable ornamental plant. In floriculture, the production of hybrids is prioritized. Hence, knowledge on floral biology and sexual reproduction of the target species is fundamental. The objectives of this study were: (1) to test sucrose concentrations and temperatures for in vitro germination of A. obesum pollen grains; (2) to identify the effect of temperature on the viability of A. obesum pollen grains; and (3) to evaluate the viability of pollen grains and stigma receptivity in pre-anthesis, at flower opening, and 72 h post-flower opening in three accessions of A. obesum. A significant relationship between temperatures and sucrose concentrations was observed in the in vitro germination test. The highest percentage of in vitro germination of pollen grains, 39.81%, was observed at an estimated temperature of 26.05 °C. Desert rose accessions maintained in biochemical oxygen demand (BOD) chambers at 30 °C during a 16-h light photoperiod showed faster flowering, and temperatures ≥ 25 °C induced pollen grain viability percentages above 69%. Temperature is one of the most important abiotic factors, influencing mainly in pollen germination, pollen tube growing and in efficiency fertilization. The ICA-wd accession stood out and can be considered a pollen donor in artificial pollination. The stigmas of flowers were receptive from a day before flower opening until three days after. The two parameters presented above, stigma receptivity and pollen viability, allow inferences about the appropriate time for successful pollination and subsequent fertilization in desert roses.

scholarly journals In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

2017 ◽  
Vol 32 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Natalia Georgieva ◽  
Ivelina Nikolova ◽  
Valentin Kosev ◽  
Yordanka Naydenova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
In Vitro Germination ◽  
Pisum Sativum L ◽  
Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

scholarly journals In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

2013 ◽  
Vol 35 (4) ◽  
pp. 1116-1126 ◽  
Author(s):  
Taliane Leila Soares ◽  
Onildo Nunes de Jesus ◽  
Janay Almeida dos Santos-Serejo ◽  
Eder Jorge de Oliveira
Keyword(s):  
Pollen Germination ◽  
Culture Medium ◽  
Pollen Viability ◽  
Pollen Grains ◽  
In Vitro Germination ◽  
Triphenyltetrazolium Chloride ◽  
Histochemical Analysis ◽  
High Viability ◽  
Three Stages

The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.

scholarly journals Longevity and germination of Juniperus communis L. pollen after storage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andrej Kormuťák ◽  
Peter Bolecek ◽  
Martin Galgóci ◽  
Dušan Gömöry
Keyword(s):  
Pollen Tube ◽  
Pollen Viability ◽  
Germination Percentage ◽  
Tube Length ◽  
In Vitro Germination ◽  
Short Term ◽  
Term Storage ◽  
Pollen Tube Length ◽  
Common Juniper

AbstractPollen storage belongs among the most important activities associated with pollen handling. It overcomes the differences in pollen shedding and ovule receptivity during controlled pollination experiments. It is especially important for species like common juniper (Juniperus communis L.) with an extremely low quality of seeds due to pollination failure. Additionally, it is a substantial part of germplasm preservation programmes in pollen banks. In the present paper, the effect of short-term storage of pollen was studied using pollen samples from five shrubs in an in vitro germination test. Two temperature regimes were tested. The pollen viability of freshly collected pollen varied considerably between individual shrubs, exhibiting 67.3–88.6% germination rate and 248.0–367.3 µm of pollen tubes. Storage at + 4 °C for four months was accompanied by a profound decline in pollen viability. The germination percentage was reduced to 49.2–75.2% and the pollen tube length to 32.5–69.0%, depending on individual shrubs. The corresponding decline in pollen viability characteristics during storage at − 20 °C was only negligible in two of the tested shrubs. In the remaining three shrub samples, an increase in germination percentage was observed. Pollen tube growth responded more sensitively to freezing, but, on average, the decrease in length was lower than that at + 4 °C. The rate of reduction in pollen tube length varied between 11.5 and 45.4%. Cytological events accompanying in vitro germination of freezer-stored pollen exhibited some delay in releasing the exine from pollen grains during the early stages of germination as compared with freshly collected pollen. In conclusion, short-term storage of the common juniper pollen in a freezer is better for the preservation of its viability than storage at + 4 °C.

scholarly journals Three-year-old Pecan Pollen Retains Fertility

1990 ◽  
Vol 115 (3) ◽  
pp. 359-363 ◽  
Author(s):  
I.E. Yates ◽  
Darrell Sparks
Keyword(s):  
Fruit Set ◽  
Pollen Viability ◽  
Storage Conditions ◽  
Reliable Indicator ◽  
In Vitro Germination ◽  
Carya Illinoensis ◽  
Pollen Storage ◽  
Fresh Pollen

Stored pollen from pecan [Carya illinoensis (Wangenh.) C. Koch] was analyzed for in vitro germination, fertilization efficiency, final fruit set, and characteristics of mature fruits. We demonstrate pecan pollen can be stored for several years and set fruit. Pollen stored for 1, 2, and 3 years at -80C and 1 year at -196C retained the capacity for fertilization. Pollen stored at -196C was more viable than pollen stored at -80C, with no significant correlation between length of storage at -80C, as judged by fruit abortions during the second drop. Final fruit set was not affected by pollen storage conditions, except for pollen collected in a season of drought. Fruit set is a more reliable indicator of pollen viability than in vitro germination. With two minor exceptions, fruits produced with stored pollen were similar to those developing after pollination with fresh pollen.

Optimising storage and in vitro germination of Eucalyptus pollen

2007 ◽  
Vol 55 (1) ◽  
pp. 83 ◽  
Author(s):  
Tasmien N. Horsley ◽  
Steven D. Johnson ◽  
Terrence K. Stanger
Keyword(s):  
Boric Acid ◽  
Pollen Germination ◽  
Storage Temperature ◽  
Sucrose Solution ◽  
Germination Rate ◽  
Pollen Viability ◽  
Eucalyptus Grandis ◽  
In Vitro Germination ◽  
Sucrose Solutions

The best sucrose solution for maximum in vitro germination of Eucalyptus pollen was investigated in order to evaluate pollen germination rate as an indicator of pollen viability. In vitro germination of both freshly collected and 1-year-old pollen (stored at 4°C) of Eucalyptus grandis, E. smithii, E. nitens, E. dunnii and E. macarthurii was carried out in 0, 10, 20, 30, 40 and 50% (w/v) sucrose solutions, either with (0.15 mg L–1) or without boric acid. Similar trends were obtained for both fresh and 1-year-old pollen, with all species responding most favourably to 30% (w/v) sucrose and 0.15 mg L–1 boric acid. When an optimal in vitro germination medium had been established, the viabilities (%germination) of E. smithii, E. nitens and E. grandis pollen, stored at room (25°C), fridge (4°C), freezer (–10°C) and liquid nitrogen (–196°C) temperatures, were compared. For all tested species, germination declined as storage temperature increased, and by 8 months, the highest survival was obtained with cryostored pollen.

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