Selection of reliable reference genes for gene expression studies using quantitative real-time PCR in navel orange fruit development and pummelo floral organs

2014 ◽  
Vol 176 ◽  
pp. 180-188 ◽  
Author(s):  
Juxun Wu ◽  
Shiying Su ◽  
Lili Fu ◽  
Yajian Zhang ◽  
Lijun Chai ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruit Development ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Navel Orange ◽  
Quantitative Real Time Pcr ◽  
Expression Studies ◽  
Orange Fruit ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Selection of reference genes for gene expression studies in rat oligodendrocytes using quantitative real time PCR

2010 ◽  
Vol 187 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Katherine Nelissen ◽  
Karen Smeets ◽  
Monique Mulder ◽  
Jerome J.A. Hendriks ◽  
Marcel Ameloot
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Ana Érika Inácio Gomes ◽  
Leonardo Prado Stuchi ◽  
Nathália Maria Gonçalves Siqueira ◽  
João Batista Henrique ◽  
Renato Vicentini ◽  
...  
Keyword(s):  
Gene Expression ◽  
Klebsiella Pneumoniae ◽  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Expression Studies ◽  
Gene Expression Studies

scholarly journals Selecting and validating reference genes for quantitative real-time PCR in Plutella xylostella (L.)

Genome ◽  
2018 ◽  
Vol 61 (5) ◽  
pp. 349-358 ◽  
Author(s):  
Yanchun You ◽  
Miao Xie ◽  
Liette Vasseur ◽  
Minsheng You
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Plutella Xylostella ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Developmental Stages ◽  
Quantitative Real Time Pcr ◽  
Experimental Conditions ◽  
Expression Studies ◽  
Gene Expression Studies

Gene expression analysis provides important clues regarding gene functions, and quantitative real-time PCR (qRT-PCR) is a widely used method in gene expression studies. Reference genes are essential for normalizing and accurately assessing gene expression. In the present study, 16 candidate reference genes (ACTB, CyPA, EF1-α, GAPDH, HSP90, NDPk, RPL13a, RPL18, RPL19, RPL32, RPL4, RPL8, RPS13, RPS4, α-TUB, and β-TUB) from Plutella xylostella were selected to evaluate gene expression stability across different experimental conditions using five statistical algorithms (geNorm, NormFinder, Delta Ct, BestKeeper, and RefFinder). The results suggest that different reference genes or combinations of reference genes are suitable for normalization in gene expression studies of P. xylostella according to the different developmental stages, strains, tissues, and insecticide treatments. Based on the given experimental sets, the most stable reference genes were RPS4 across different developmental stages, RPL8 across different strains and tissues, and EF1-α across different insecticide treatments. A comprehensive and systematic assessment of potential reference genes for gene expression normalization is essential for post-genomic functional research in P. xylostella, a notorious pest with worldwide distribution and a high capacity to adapt and develop resistance to insecticides.

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