LED lights increase bioactive substances at low energy costs in culturing fruiting bodies of Cordyceps militaris

2014 ◽  
Vol 175 ◽  
pp. 139-143 ◽  
Author(s):  
Zhi-Li Yi ◽  
Wen-Fang Huang ◽  
Yan Ren ◽  
Eugen Onac ◽  
Guo-Fu Zhou ◽  
...  
Keyword(s):  
Cordyceps Militaris ◽  
Low Energy ◽  
Energy Costs ◽  
Fruiting Bodies ◽  
Bioactive Substances ◽  
Led Lights

scholarly journals Low Dose of Fluoride in the Culture Medium of Cordyceps militaris Promotes Its Growth and Enhances Bioactives with Antioxidant and Anticancer Properties

2021 ◽  
Vol 7 (5) ◽  
pp. 342
Author(s):  
Xiaoshuai Li ◽  
Jia Wang ◽  
Huayue Zhang ◽  
Long Xiao ◽  
Zhongfang Lei ◽  
...  
Keyword(s):  
Low Dose ◽  
Radical Scavenging ◽  
Potassium Fluoride ◽  
Cordyceps Militaris ◽  
Carotenoid Content ◽  
Market Demand ◽  
Fruiting Bodies ◽  
Human Osteosarcoma Cells ◽  
Wide Range ◽  
Bioactive Ingredients

Cordyceps militaris possesses several compounds with medicinal properties, and is commonly used in traditional Chinese functional food and medicine for a variety of health benefits. Because of its rare occurrence in nature, the market demand for artificial C. militaris is on the rise. Furthermore, efforts to increase its bioactive ingredients have also been considered in research. In this study, we aimed to investigate the effect of fluoride on the growth and enrichment of bioactive compounds in C. militaris. A wide range of potassium fluoride concentrations (0, 0.001, 0.01, 0.1, and 1 mM) were added to the culture media as a source of fluoride during the cultivation of C. militaris fruiting bodies. The contents of fluorine and bioactive substances of the fruiting bodies in normal (NM) and fluorine-supplemented (FM) media were measured and compared. C. militaris raised in the growth medium supplemented with 0.01 mM potassium fluoride led to a 44.86% (1.55 ± 0.14 g/bottle) increase in biomass and a 23.43% (3161.38 ± 35.71 µg/g) increase in total carotenoid content in the fruiting bodies. Furthermore, a remarkable increase in superoxide dismutase-like activity (84.75 U/mg) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (IC50 = 2.59 mg/mL) was recorded. In human cancer cell-based assays, C. militaris raised in FM caused stronger cytotoxicity, apoptosis, and cell cycle arrest in human osteosarcoma cells. These results demonstrated that a low dose of fluoride could stimulate the growth of C. militaris fruiting bodies and enhance the production of bioactive ingredients that possess useful antioxidant and anticancer activities.

Structure and properties of powders obtained by electrodispersion of corrosion-resistant metal waste in kerosine

2021 ◽  
pp. 276-281
Author(s):  
E.V. Ageeva ◽  
E.V. Ageev ◽  
A.A. Sysoev
Keyword(s):  
High Efficiency ◽  
Experimental Studies ◽  
Low Energy ◽  
Powder Materials ◽  
Energy Costs ◽  
Structure And Properties ◽  
Corrosion Resistant ◽  
High Chromium ◽  
Metal Waste ◽  
Industrial Use

The results of experimental studies of the structure and properties of electroerosive high-chromium powders obtained in kerosene are presented. The high efficiency of using the electrodispersing technology is shown, which provides for obtaining new corrosion-resistant powder materials suitable for industrial use at low energy costs.

Protective Effects on Mitochondria and Anti-Aging Activity of Polysaccharides from Cultivated Fruiting Bodies of Cordyceps militaris

2010 ◽  
Vol 38 (06) ◽  
pp. 1093-1106 ◽  
Author(s):  
Xing-Tai Li ◽  
Hong-Cheng Li ◽  
Chun-Bin Li ◽  
De-Qiang Dou ◽  
Ming-Bo Gao
Keyword(s):  
Hydroxyl Radicals ◽  
Cordyceps Militaris ◽  
Mitochondrial Swelling ◽  
Dependent Manner ◽  
Protective Effects ◽  
Fruiting Bodies ◽  
Concentration Dependent Manner ◽  
Mitochondrial Injury

Cordyceps militaris (L.) Link is an entomopathogenic fungus parasitic to Lepidoptera larvae, and is widely used as a folk tonic or invigorant for longevity in China. Although C. militaris has been used in traditional Chinese medicine for millennia, there is still a lack convincing evidence for its anti-aging activities. This study was performed to investigate the effects of polysaccharides from cultivated fruiting bodies of C. militaris (CMP) on mitochondrial injury, antioxidation and anti-aging activity. Fruiting bodies of C. militaris were cultivated artificially under optimized conditions. The spectrophotometric method was used to measure thiobarbituric acid reactive substances (TBARS), mitochondrial swelling, and activities of scavenging superoxide anions in vitro. D-galactose (100 mg/kg/day) was injected subcutaneously into back of the neck of mice for 7 weeks to induce an aging model. The effects of CMP on the activities of catalase (CAT), surperoxide dismutase (SOD), glutathione peroxidase (GPx) and anti-hydroxyl radicals were assayed in vivo using commercial monitoring kits. The results showed that CMP could inhibit mitochondrial injury and swelling induced by Fe2+ -L-Cysteine in a concentration- dependent manner and it also had a significant superoxide anion scavenging effect. Moreover, the activities of CAT, SOD, GPx and anti-hydroxyl radicals in mice liver were increased significantly by CMP. These results indicate that CMP protects mitochondria by scavenging reactive oxygen species (ROS), inhibiting mitochondrial swelling, and increasing the activities of antioxidases. Therefore, CMP may have pharmaceutical values for mitochondrial protection and anti-aging. CMP was the major bioactive component in C. militaris.

scholarly journals Conversion of a microwave synthesized alkali-metal MOF to a carbonaceous anode for Li-ion batteries

RSC Advances ◽  
2020 ◽  
Vol 10 (23) ◽  
pp. 13732-13736
Author(s):  
Aamod V. Desai ◽  
Vanessa Pimenta ◽  
Cara King ◽  
David B. Cordes ◽  
Alexandra M. Z. Slawin ◽  
...  
Keyword(s):  
Alkali Metal ◽  
Low Energy ◽  
Rechargeable Battery ◽  
Microwave Assisted Synthesis ◽  
Energy Costs ◽  
Li Ion Batteries ◽  
Microwave Assisted ◽  
Li Ion

An alkali-metal MOF is prepared using microwave-assisted synthesis, which is converted into a carbonaceous solid at low energy costs. The MOF-derived solid functions as a promising anode for Li-ion rechargeable battery (LIB).

DEVELOPMENT OF TECHNOLOGY FOR ELECTROEROSIVE COPPER NANOPARTICLES FROM WASTE

2016 ◽  
Vol 3 (1) ◽  
pp. 520-524
Author(s):  
Маховицкий ◽  
E. Makhovitskiy ◽  
Агеев ◽  
E. Ageev ◽  
Хорьякова ◽  
...  
Keyword(s):  
Experimental Research ◽  
Copper Nanoparticles ◽  
Copper Wire ◽  
Low Energy ◽  
Energy Costs ◽  
Conductive Material ◽  
Technology Of Production ◽  
Electroerosion Dispersion

One of the most promising methods of processing of practically any conductive material, characterized by relatively low energy costs and environmental cleanliness of the process, is the method of electroerosion dispersion. However, to obtain the electro erosion dispersing of nanoparticles it is necessary to conduct a comprehensive theoretical and experimental research. The article describes the technology of production electroerosion copper nanoparticles from the waste electrical copper wire.

scholarly journals Impacto de LoRaWAN no Desempenho de Plataformas de IoT baseadas em Nuvem e Névoa Computacional

2019 ◽  
Author(s):  
Ivan Zyrianoff ◽  
Alexandre Heideker ◽  
Dener Ottolini Silva ◽  
João Henrique Kleinschmidt ◽  
Carlos Alberto Kamienski
Keyword(s):  
Performance Evaluation ◽  
System Performance ◽  
New Technology ◽  
Fog Computing ◽  
Data Communication ◽  
Evaluation Study ◽  
Low Energy ◽  
Energy Costs ◽  
Computational Aspects ◽  
Iot Platforms

LoRaWAN is a new technology that has been consolidating as a key data communication component to send data in IoT-based systems, due to its ability to send data over long distances with low energy costs. However, literature considers only wireless aspects, disregarding its computational aspects and its integration with IoT platforms, as well as ignoring the deployment possibilities that involve cloud and fog computing. In order to understand the computational impacts of the LoRa architecture we performed a careful performance evaluation study in a complex IoT scenario, exploring cloud and fog computing scenarios and integrating with the IoT FIWARE platform. The results show that the LoRaWAN architecture is scalable, but it has impacts on system performance.

scholarly journals INVESTIGATION ON FRUITING BODY OF CORDYCEPS MILITARIS CULTIVATION IN TRA VINH

2019 ◽  
Vol 1 (27) ◽  
pp. 95-103
Author(s):  
Trai Ngoc Nguyen ◽  
Nam Quoc Phan ◽  
SaNa Que Son ◽  
Dang Thi Thao Tran
Keyword(s):  
Mass Production ◽  
Brown Rice ◽  
Cordyceps Militaris ◽  
Cordyceps Sinensis ◽  
Effect Of Temperature ◽  
Fruiting Bodies ◽  
Silkworm Pupae ◽  
Low Mass ◽  
Rice Medium ◽  
Biochemical Components

Cordyceps sinensis has traditionally been used in traditional Chinese medicine. However, C. sinensis has very low mass production and only grows in natural environment, whereas the fruiting bodies of Cordyceps militaris can be successfully farmed in cereals. C.  militaris contains similar biochemical components as C. sinensis as well as the anticancer component cordycepin. This study is to investigate the effect of temperature, light intensity, milling of silkworm pupae  supplemented with brown rice medium and silkworm pupae age on fruiting bodies of C. militaris. The optimal temperature for the formation of fruiting bodieswas 25oC, under conditions of 500 lux lighting. A bottle with 50 g of brown rice supplemented with 50 ml of distilled water and 5 g of milling silkworm pupae  produced 20,11 fruiting bodies with the weight of 10.14 g, Cordycepin and Adenosine obtained were 10.08 mg/g and 0.57 mg/g, respectively. From mounting, the nine-dayold pupae showed the best incidence of infection and development of fruiting bodies of C. militaris with 1.69 fruiting bodies/pupae.

scholarly journals Integrative analysis of selected metabolites and the fungal transcriptome during the developmental cycle of Ganoderma lucidum strain G0119 correlates lignocellulose degradation with carbohydrate and triterpenoid metabolism

2021 ◽  
Author(s):  
Shuai Zhou ◽  
Xiaoyu Zhang ◽  
Fuying Ma ◽  
Shangxian Xie ◽  
Chuanhong Tang ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Ganoderma Lucidum ◽  
Lignocellulose Degradation ◽  
Fruiting Bodies ◽  
Enzyme Expression ◽  
Fruiting Body Formation ◽  
Bioactive Substances ◽  
Body Formation ◽  
Substrate Degradation ◽  
Polysaccharide Synthesis

To systemically understand the biosynthetic pathways of bioactive substances, including triterpenoids and polysaccharides, in Ganoderma lucidum, the correlation between substrate degradation, carbohydrate and triterpenoid metabolism during growth was analyzed by combining changes in metabolite content and changes in related enzyme expression in G. lucidum over 5 growth phases. Changes in low-polarity triterpenoid content were correlated with changes in glucose and mannitol content in fruiting bodies. Additionally, changes in medium-polarity triterpenoid content were correlated with changes in the lignocellulose content of the substrate and with the glucose, trehalose and mannitol contents of fruiting bodies. Weighted gene coexpression network analysis (WGCNA) indicated that changes in trehalose and polyol content were related to carbohydrate catabolism and polysaccharide synthesis. Changes in triterpenoid content were related to expression of the carbohydrate catabolic enzymes, laccase, cellulase, hemicellulase, and polysaccharide synthase and to the expression of several cytochrome P450 monooxygenases (CYPs). It was concluded that the products of cellulose and hemicellulose degradation participate in polyol, trehalose and polysaccharide synthesis during initial fruiting body formation. These carbohydrates accumulate in the early phase of fruiting body formation and are utilized when the fruiting bodies mature and a large number of spores are ejected. An increase in carbohydrate metabolism provides additional precursors for the synthesis of triterpenoids. Importance Most studies of G. lucidum have focused on its medicinal function and on the mechanism of its activity, whereas the physiological metabolism and synthesis of bioactive substances during the growth of this species have been less studied. Therefore, theoretical guidance for cultivation methods to increase the production of bioactive compounds remains lacking. This study integrated changes in the lignocellulose, carbohydrate and triterpenoid contents of G. lucidum with enzyme expression from transcriptomics data using WGCNA. The findings helped us better understand the connections between substrate utilization and the synthesis of polysaccharides and triterpenoids during the cultivation cycle of G. lucidum. The results of WGCNA suggest that the synthesis of triterpenoids can be enhanced not only through regulating the expression of enzymes in the triterpenoid pathway, but also through regulating carbohydrate metabolism and substrate degradation. This study provides a potential approach and identifies enzymes that can be targeted to regulate lignocellulose degradation and accelerate the accumulation of bioactive substances by regulating substrate degradation in G. lucidum.

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