Evaluation of a laboratory scale conventional shake flask and a bioreactor on cell growth and regeneration of banana cell suspension cultures

2014 ◽  
Vol 172 ◽  
pp. 39-46 ◽  
Author(s):  
Wendy Yi Wen Chin ◽  
Mohamad Suffian Mohamad Annuar ◽  
Boon Chin Tan ◽  
Norzulaani Khalid
Keyword(s):  
Cell Growth ◽  
Cell Suspension ◽  
Shake Flask ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Laboratory Scale

scholarly journals Flavonoid Accumulation in Cell Suspension Cultures of Glycyrrhiza inflata Batal under Optimizing Conditions

2009 ◽  
Vol 64 (1-2) ◽  
pp. 68-72 ◽  
Author(s):  
Ying Yang ◽  
Feng He ◽  
Longjiang Yu ◽  
Jiaxing Ji ◽  
Yezhen Wang
Keyword(s):  
Cell Growth ◽  
Cell Suspension ◽  
Cell Cultures ◽  
Large Scale ◽  
Cell Suspension Cultures ◽  
Optimization Method ◽  
Suspension Cultures ◽  
Initial Inoculum ◽  
Nitrogen Concentrations ◽  
Glycyrrhiza Inflata

Cell growth and flavonoid production in cell suspension cultures of Glycyrrhiza inflata Batal were investigated under various initial inoculum densities, and sucrose and nitrogen concentrations to develop an optimization method for an improved flavonoid production. Both biomass accumulation and flavonoid production exhibited an “S” curve in one culture cycle, with the greatest value obtained on day 21, which showed that cell growth and fl avonoid biosynthesis went along isochronously. Moreover, according to the biomass and flavonoid production, the appreciate inoculum density, and the sucrose and nitrogen concentrations were 50 g FW L-1, 50 g L-1 and 120 mmol L-1, respectively. In addition, cell growth and flavonoid production showed a peak of 16.4 g DW L-1 and 95.7 mg L-1 on day 21 under the optimizing conditions, respectively. The flavonoid productivity of the cells which were cultured for 3 years is higher than that of the 3-year-old plant, which suggested that flavonoid production by cell cultures of G. inflata is a potentially profitable method. Therefore, this work is considered to be helpful for efficient large-scale bioprocessing of cell cultures in bioreactors.

Xad-4 Elicitation Effects on Cell Growth and Sugar Uptake in Cell Suspension Cultures ofVetiveria ZizanioidesStapf. (Poaceae)

1994 ◽  
Vol 128 (1) ◽  
pp. 294-294 ◽  
Author(s):  
M. Mucciarelli ◽  
M. Cozzo ◽  
M. Gallino ◽  
S. Scannerini ◽  
A. Bocco ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Sugar Uptake
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