Seasonal changes in abiotic stress tolerance and concentrations of tocopherol, sugar, and ascorbic acid in sea buckthorn leaves and stems

2013 ◽  
Vol 164 ◽  
pp. 232-237 ◽  
Author(s):  
Yoshinori Kanayama ◽  
Kazuyoshi Sato ◽  
Hiroki Ikeda ◽  
Tomoko Tamura ◽  
Manabu Nishiyama ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Seasonal Changes ◽  
Abiotic Stress Tolerance ◽  
Sea Buckthorn

scholarly journals Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation

2019 ◽  
Author(s):  
Shoaib Munir ◽  
Muhammad Ali Mumtaz ◽  
John Kojo Ahiakpa ◽  
Genzhong Liu ◽  
Wei Zheng ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Abiotic Stress ◽  
Gene Family ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Light Response ◽  
Genome Wide ◽  
Genes Encoding ◽  
Transgenic Lines ◽  
Distribution Mapping

Abstract Background Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear. Results In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. In addition, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that AsA biosynthesis was mainly improved by Myo-inositol pathway in leaves and fruits. MIOX4 overexpression lines exhibited a betterlight response, abiotic stress tolerance, and AsA biosynthesis capacity. Conclusions These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of MIOX gene family and their involvement in ascorbate biosynthesis in tomato.

scholarly journals Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation

2020 ◽  
Author(s):  
Shoaib Munir ◽  
Muhammad Ali Mumtaz ◽  
John Kojo Ahiakpa ◽  
Genzhong Liu ◽  
Weifang Chen ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Abiotic Stress ◽  
Gene Family ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Light Response ◽  
Genome Wide ◽  
Genes Encoding ◽  
Transgenic Lines ◽  
Distribution Mapping

Abstract Background Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants, and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, Myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear. Results In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. Besides, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of the MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that the Myo-inositol pathway improved the AsA biosynthesis in leaves and fruits. MIOX4 overexpression lines exhibited abetter light response, abiotic stress tolerance, and AsA biosynthesis capacity. Conclusions These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of the MIOX gene family and their involvement in ascorbate biosynthesis in tomato.

scholarly journals Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation

2020 ◽  
Author(s):  
Shoaib Munir ◽  
Muhammad Ali Mumtaz ◽  
John Kojo Ahiakpa ◽  
Genzhong Liu ◽  
Wei Zheng ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Abiotic Stress ◽  
Gene Family ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Light Response ◽  
Genome Wide ◽  
Genes Encoding ◽  
Transgenic Lines ◽  
Distribution Mapping

Abstract Background: Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants, and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, Myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear. Results : In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. Besides, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of the MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that the Myo-inositol pathway improved the AsA biosynthesis in leaves and fruits. MIOX4 overexpression lines exhibited a better light response, abiotic stress tolerance, and AsA biosynthesis capacity. Conclusions: These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of the MIOX gene family and their involvement in ascorbate biosynthesis in tomato.

Abiotic Stress Tolerance in Plants by Priming and Pretreatments with Ascorbic Acid

2019 ◽  
pp. 459-493
Author(s):  
Sajid Ali ◽  
Aamir Nawaz ◽  
Sajjad Hussain ◽  
Samiya Mahmood Khan ◽  
Shaghef Ejaz ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance

Calcium-Dependent Protein Kinases (CDPK) in Abiotic Stress Tolerance

2018 ◽  
Vol 34 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Hemant B Kardile ◽  
◽  
Vikrant ◽  
Nirmal Kant Sharma ◽  
Ankita Sharma ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Protein Kinases ◽  
Abiotic Stress Tolerance ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

scholarly journals Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 23
Author(s):  
Antt Htet Wai ◽  
Muhammad Waseem ◽  
A B M Mahbub Morshed Khan ◽  
Ujjal Kumar Nath ◽  
Do Jin Lee ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Gene Family ◽  
Stress Tolerance ◽  
Solanum Lycopersicum ◽  
Expression Profiling ◽  
Abiotic Stress Tolerance ◽  
Dependent Manner ◽  
Solanum Lycopersicum L ◽  
Genome Wide ◽  
Protein Disulfide

Protein disulfide isomerases (PDI) and PDI-like proteins catalyze the formation and isomerization of protein disulfide bonds in the endoplasmic reticulum and prevent the buildup of misfolded proteins under abiotic stress conditions. In the present study, we conducted the first comprehensive genome-wide exploration of the PDI gene family in tomato (Solanum lycopersicum L.). We identified 19 tomato PDI genes that were unevenly distributed on 8 of the 12 tomato chromosomes, with segmental duplications detected for 3 paralogous gene pairs. Expression profiling of the PDI genes revealed that most of them were differentially expressed across different organs and developmental stages of the fruit. Furthermore, most of the PDI genes were highly induced by heat, salt, and abscisic acid (ABA) treatments, while relatively few of the genes were induced by cold and nutrient and water deficit (NWD) stresses. The predominant expression of SlPDI1-1, SlPDI1-3, SlPDI1-4, SlPDI2-1, SlPDI4-1, and SlPDI5-1 in response to abiotic stress and ABA treatment suggested they play regulatory roles in abiotic stress tolerance in tomato in an ABA-dependent manner. Our results provide new insight into the structure and function of PDI genes and will be helpful for the selection of candidate genes involved in fruit development and abiotic stress tolerance in tomato.

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