Influence of nutrient deficiency on root architecture and root hair morphology of trifoliate orange (Poncirus trifoliata L. Raf.) seedlings under sand culture

2013 ◽  
Vol 162 ◽  
pp. 100-105 ◽  
Author(s):  
Xiu Cao ◽  
Chunli Chen ◽  
Dejian Zhang ◽  
Bo Shu ◽  
Jie Xiao ◽  
...  
Keyword(s):  
Root Hair ◽  
Root Architecture ◽  
Nutrient Deficiency ◽  
Poncirus Trifoliata ◽  
Sand Culture ◽  
Trifoliate Orange ◽  
Hair Morphology

scholarly journals Exogenous Phytohormones Modulate Mycorrhiza-Induced Changes in Root Hair Configuration of Trifoliate Orange

2016 ◽  
Vol 44 (2) ◽  
pp. 548-556 ◽  
Author(s):  
Chun-Yan LIU ◽  
A.K. SRIVASTAVA ◽  
De-Jian ZHANG ◽  
Ying-Ning ZOU ◽  
Qiang-Sheng Wu
Keyword(s):  
Root Hair ◽  
Lateral Roots ◽  
Poncirus Trifoliata ◽  
Hair Length ◽  
Trifoliate Orange ◽  
Hair Density ◽  
Root Hair Length ◽  
Nylon Mesh ◽  
Root Hair Density ◽  
Induced Changes

Mycorrhizas alter root hair profile, but it is not clear whether exogenous phytohormones regulate the mycorrhizal effects on root hair. Studies were carried out in a two-chambered rootbox separated by 37-μm nylon mesh to establish root+hyphae chamber carrying trifoliate orange [Poncirus trifoliata (L.) Raf.] as the test plant inoculated with Diversispora versiformis and hyphae chamber (without roots). Indole butyric acid (IBA), abscisic acid (ABA), and jasmonic acid (JA) (each at 0.1 μM concentration) were weekly applied into hyphae chamber, in total of six times before plant harvest. Mycorrhization strongly stimulated plant growth performance, and exogenous phytohormones, especially IBA, further magnified the mycorrhizal-stimulated growth responses. Three exogenous phytohormones decreased mycorrhizal colonization in taproot and first-order lateral roots, but increased in second- and third-order lateral roots. These phytohormones also increased hyphal length in nylon mesh and soil, irrespective of root+hyphae or hyphae chamber. Mycorrhizal inoculation significantly increased root hair density of different root classes, and exogenous hormones further strengthened the mycorrhizal effect. Average root hair length was stimulated by mycorrhization, but all exogenous phytohormones weakened the mycorrhizal response. Mycorrhization in combination with exogenous phytohormones showed no response on root hair diameter. Our studies, hence, suggested that application of exogenous phytohormones in hyphae chamber strengthened the D. versiformis-induced changes in average root hair density but weakened in average root hair length in trifofliate orange grown in root+hyphae chamber.

scholarly journals Transcriptional changes in CiFT-introduced transgenic trifoliate orange (Poncirus trifoliata L. Raf.)

2010 ◽  
Vol 30 (3) ◽  
pp. 431-439 ◽  
Author(s):  
F. Nishikawa ◽  
T. Endo ◽  
T. Shimada ◽  
H. Fujii ◽  
T. Shimizu ◽  
...  
Keyword(s):  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Transcriptional Changes

scholarly journals Community Analysis of Arbuscular Mycorrhizal Fungi in Roots ofPoncirus trifoliataandCitrus reticulataBased on SSU rDNA

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Wang ◽  
Yin Wang
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Small Subunit ◽  
Poncirus Trifoliata ◽  
Spore Density ◽  
Morphological Observation ◽  
Trifoliate Orange ◽  
Hyphal Length

Morphological observation of arbuscular mycorrhizal fungi (AMF) species in rhizospheric soil could not accurately reflect the actual AMF colonizing status in roots, while molecular identification of indigenous AMF colonizing citrus rootstocks at present was rare in China. In our study, community of AMF colonizing trifoliate orange (Poncirus trifoliataL. Raf.) and red tangerine (Citrus reticulataBlanco) were analyzed based on small subunit of ribosomal DNA genes. Morphological observation showed that arbuscular mycorrhizal (AM) colonization, spore density, and hyphal length did not differ significantly between two rootstocks. Phylogenetic analysis showed that 173 screened AMF sequences clustered in at least 10 discrete groups (GLO1~GLO10), all belonging to the genus ofGlomusSensu Lato. Among them, GLO1 clade (clustering with uncultured Glomus) accounting for 54.43% clones was the most common in trifoliate orange roots, while GLO6 clade (clustering withGlomus intraradices) accounting for 35.00% clones was the most common in red tangerine roots. Although, Shannon-Wiener indices exhibited no notable differences between both rootstocks, relative proportions of observed clades analysis revealed that composition of AMF communities colonizing two rootstocks varied severely. The results indicated that native AMF species in citrus rhizosphere had diverse colonization potential between two different rootstocks in the present orchards.

scholarly journals Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

10.3791/55971 ◽  
2017 ◽  
Author(s):  
Jayde A. Aufrecht ◽  
Jennifer M. Ryan ◽  
Sahar Hasim ◽  
David P. Allison ◽  
Andreas Nebenführ ◽  
...  
Keyword(s):  
Root Hair ◽  
Microfluidic Platform ◽  
Hair Morphology

scholarly journals ERF109 of trifoliate orange (Poncirus trifoliata(L.) Raf.) contributes to cold tolerance by directly regulating expression ofPrx1involved in antioxidative process

2019 ◽  
Vol 17 (7) ◽  
pp. 1316-1332 ◽  
Author(s):  
Min Wang ◽  
Wenshan Dai ◽  
Juan Du ◽  
Ruhong Ming ◽  
Bachar Dahro ◽  
...  
Keyword(s):  
Cold Tolerance ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange

scholarly journals High Sucrose Levels and Abnormal Enzyme Activity as a Function of Nutritional Stress in Sugarcane

1969 ◽  
Vol 51 (4) ◽  
pp. 325-333
Author(s):  
Alex G. Alexander
Keyword(s):  
Nutrient Deficiency ◽  
Critical Factor ◽  
Nutritional Stress ◽  
Nutrient Supply ◽  
Oxidative Enzymes ◽  
Sand Culture ◽  
High Level ◽  
To Receive ◽  
High Sucrose ◽  
Nitrogen Phosphorus

Deficiencies of nitrogen, phosphorus, potassium, and calcium were gradually induced in sugarcane grown by sand culture. There were three objectives: 1, To determine whether nutritional stress in general is a means of inducing higher sucrose production; 2, to determine whether sucrose increases can be triggered without reaching nutrient-deficiency levels which would reduce tonnage; and 3, to ascertain whether increased sucrose production can be traced to common behavior patterns of hydrolytic and oxidative enzymes. All plants received a moderately high level of all nutrients until 14 weeks of age. Thereafter, individual nutrients were gradually lowered at intervals of 2 weeks, while control plants continued to receive the original levels. All plants subjected to nutritional stress accumulated higher leaf sucrose than control plants, regardless of the nutrient withheld. In each instance sucrose increases were recorded before nutrient supply had reached deficiency proportions. General nutritional stress thus appears to trigger variations in sugar level, with nutritional imbalance rather than actual deficiency being a critical factor. All plants under nutritional stress revealed a concurrent suppression of amylase as sucrose increased. Peroxidase was greatly stimulated by all nutritional treatments. Phosphatase was suppressed by decreasing nitrate and phosphorus, but was generally unaffected by the potassium and calcium treatments. Several distinct mechanisms of increased sucrose formation are indicated by the enzyme data. Sensitivity of amylase to changing nutrient supply is discussed from the standpoint of a possible indicator of approaching deficiency.

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