Ex vitro symbiotic seed germination of Spathoglottis plicata Blume on common orchid cultivation substrates

2013 ◽  
Vol 160 ◽  
pp. 238-242 ◽  
Author(s):  
Natdanai Aewsakul ◽  
Daroonsri Maneesorn ◽  
Phanu Serivichyaswat ◽  
Auttapon Taluengjit ◽  
Sureeporn Nontachaiyapoom
2017 ◽  
Vol 58 (1) ◽  
Author(s):  
Xu Zeng ◽  
Yuanyuan Li ◽  
Hong Ling ◽  
Sisi Liu ◽  
Mengmeng Liu ◽  
...  

2016 ◽  
Vol 8 (9) ◽  
pp. 168
Author(s):  
Fatemeh Feizi ◽  
Mousa Mousavi

<p>The main propagation method of <em>Cassia fistula</em> is sowing seeds. The seed germination is usually low because of its impermeable hard coat. Therefore, this experiment evaluated the effects of TiO<sub>2</sub> nanoparticles and scarification methods on seed germination and seedling growth <em>in vitro</em> condition. The tree seeds were treated with, hot water, H<sub>2</sub>SO<sub>4</sub> (36N), and mechanical scarification and culture on ¼ MS salt mixture. The medium was supplemented with different concentrations of TiO<sub>2</sub> nanoparticles. The results showed that the highest percentage and rate of germination was recorded in seeds treated with mechanical scarification. The highest shoot and root dry weight was recorded for seeds treated with mechanical scarification and grown on MS media supplemented with 1.5 mg/ml TiO<sub>2</sub> nanoparticles. TiO<sub>2</sub> nanoparticles did not show any significant effects on the percentage and rate of germination. Different growing soil mixtures had significant effects on the growth of the ex vitro transferred plantlets. Coco peat and peat moss mixture (1:1) was found to be more effective in increasing the number of leaves and root length of the seedlings.</p>


HortScience ◽  
2007 ◽  
Vol 42 (1) ◽  
pp. 135-139 ◽  
Author(s):  
Lawrence W. Zettler ◽  
Sarah B. Poulter ◽  
Kris I. McDonald ◽  
Scott L. Stewart

Seeds of an endangered epiphytic orchid from Florida (Epidendrum nocturnum Jacquin) germinated in vitro with a mycorrhizal fungus [Epulorhiza repens (Bernard) Moore] using a technique normally applied to terrestrial orchids (symbiotic seed germination). Seeds from two sources (Fakahatchee Strand, Fla. Panther NWR) were sown on either modified oats medium (MOM) or standard oat medium (SOM) and inoculated with the fungus. Significant differences in germination were detected between the two seed sources. MOM had a significant effect on mean leaf length during incubation in vitro (F (1278) = 23.81, P > 0.000), but media had no significant effect on leaf number. After 48 days in vitro, all leaf-bearing seedlings were exposed to light and then transferred to greenhouse conditions ex vitro on sterile Sphagnum moss with or without half-strength Miracle-Gro (Scotts, Port Washington, N.Y.) commercial fertilizer. After 163 days ex vitro, seedlings on Sphagnum without Miracle-Gro displayed highest survivorship (>90%), whereas Miracle-Gro-exposed seedlings from standard oat agar experienced low (44%) survivorship. Healthy seedlings with a mycotrophic capability were obtained 1 year after sowing. A total of 43 seedlings were subsequently reintroduced into the Florida Panther NWR in Nov. 2005, 16 months after sowing. The symbiotic technique may, therefore, have practical merit for conservation of E. nocturnum and other epiphytic orchids threatened with extinction.


2003 ◽  
Vol 149 (1) ◽  
pp. 104-120 ◽  
Author(s):  
JYOTSNA SHARMA ◽  
LAWRENCE W. ZETTLER ◽  
J. W. VAN SAMBEEK ◽  
MARK R. ELLERSIECK ◽  
CHRISTOPHER J. STARBUCK

2007 ◽  
Vol 90 (3) ◽  
pp. 313-323 ◽  
Author(s):  
Timothy R. Johnson ◽  
Scott L. Stewart ◽  
Daniela Dutra ◽  
Michael E. Kane ◽  
Larry Richardson

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