Molecular polymorphism and genetic relationships in date palm (Phoenix dactylifera L.): The utility of nuclear microsatellite markers

2012 ◽  
Vol 148 ◽  
pp. 255-263 ◽  
Author(s):  
Salwa Zehdi ◽  
Emira Cherif ◽  
Soumaya Rhouma ◽  
Sylvain Santoni ◽  
Amel Salhi Hannachi ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Date Palm ◽  
Genetic Relationships ◽  
Phoenix Dactylifera ◽  
Molecular Polymorphism ◽  
Phoenix Dactylifera L ◽  
Nuclear Microsatellite

Genetic relationships and genotype tracing in date palms (Phoenix dactylifera L.) in Oman, based on microsatellite markers

2008 ◽  
Vol 6 (1) ◽  
pp. 70-72 ◽  
Author(s):  
Ishaq Ahmed Al-Ruqaishi ◽  
Michael Davey ◽  
Peter Alderson ◽  
Sean Mayes
Keyword(s):  
Microsatellite Markers ◽  
Date Palm ◽  
Size Range ◽  
Genetic Relationships ◽  
Phoenix Dactylifera ◽  
Amplification Product ◽  
Phoenix Dactylifera L ◽  
Expected Size Range ◽  
Close Relationship ◽  
Clonal Lines

Microsatellite markers (SSRs) were used to screen and analyse the genetic diversity among clonal genotypes of date palm (Phoenix dactylifera L.) derived by somatic embryogenesis in Oman. Twenty-one palms, representing 14 Omani, five Bahraini, one Iraqi and one Moroccan genotype, were screened with ten microsatellite markers. All primer pairs produced an amplification product in the expected size range and detected high levels of polymorphism among the analysed samples. Correspondence analysis revealed that the genotypes from Bahrain and Iraq showed a close relationship with accessions already grown in Oman. The genotype from Morocco (Medjool) appeared distinct from the rest of the material. Three independent clonal lines derived from a single Khalas Aldahra genotype were found to give identical genetic fingerprints. The value of this work for date palm production and conservation in Oman is discussed.

scholarly journals DEVELOPMENT OF 1000 MICROSATELLITE MARKERS ACROSS THE DATE PALM (PHOENIX DACTYLIFERA L.) GENOME

2010 ◽  
pp. 269-277 ◽  
Author(s):  
A. Hamwieh ◽  
J. Farah ◽  
S. Moussally ◽  
K. Al-Sham'aa ◽  
K. Almer ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L

scholarly journals Assessment of AFLP-based Genetic Relationships among Date Palm (Phoenix dactylifera L.) Varieties of Iraq

2005 ◽  
Vol 130 (3) ◽  
pp. 442-447 ◽  
Author(s):  
Jaladet M.S. Jubrael ◽  
Sripada M. Udupa ◽  
Michael Baum
Keyword(s):  
Date Palm ◽  
Genetic Relationships ◽  
Similarity Index ◽  
Phoenix Dactylifera ◽  
Group Method ◽  
Pair Group ◽  
Phoenix Dactylifera L ◽  
Varietal Identification ◽  
Average Proportion

Currently, the identification and characterization of date palm varieties rely on a small number of morphological traits, mainly of fruit, which are complex and greatly influenced by the environment. As a result, different varietal names may actually refer to the same variety while different varieties may have the same name. Therefore, new descriptors like molecular markers are required to identify, characterize, and estimate genetic diversity in this crop. Here we used amplified fragment length polymorphism (AFLP) markers to discriminate 18 Iraqi date palm varieties and to estimate the genetic relationship among the varieties. A total of 122 polymorphic AFLP loci were scored, with an average of 17.4 polymorphic loci per primer combination. The use of any one of the four combinations, P101(aacg)/M95(aaaa), P74(ggt)/M95(aaaa), P73(ggg)/M95(aaaa), or P100(aacc)/M95(aaaa), was sufficient to uniquely identify all the varieties. Jaccard's genetic similarity index ranged from 0.108 to 0.756, indicating moderate to diverse relationships. Estimation of average proportion of fixed recessive AFLP loci indicated that most of the loci in variety `Chipchab' were fixed, whereas most of the loci in `Jamal Al-Dean' could be heterozygous and in-between in other varieties. Unweighted pair group method with arithmatic mean (UPGMA) analysis ordered the date palm varieties first into two broad groups at 27% similarity levels. One group consisted of seven varieties and the second group consisted of the remaining 11 varieties of date palm. These results showed that the AFLP technique is an efficient method for varietal identification and estimating genetic relationships in date palm.

scholarly journals New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)

3 Biotech ◽  
2011 ◽  
Vol 1 (2) ◽  
pp. 91-97 ◽  
Author(s):  
Khaled Elmeer ◽  
Hina Sarwath ◽  
Joel Malek ◽  
Michael Baum ◽  
Aladdin Hamwieh
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L
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