Magnetic exposure improves tolerance of fig ‘Sabz’ explants to drought stress induced in vitro

2012 ◽  
Vol 137 ◽  
pp. 95-99 ◽  
Author(s):  
Soheil Karimi ◽  
Shadi Hojati ◽  
Saeid Eshghi ◽  
Rahim Nazary Moghaddam ◽  
Samira Jandoust
Keyword(s):  
Drought Stress ◽  
Magnetic Exposure

scholarly journals Effect of drought stress on in vitro neutral detergent fiber digestibility of corn for silage

2021 ◽  
Vol 273 ◽  
pp. 114803
Author(s):  
G. Ferreira ◽  
L.L. Martin ◽  
C.L. Teets ◽  
B.A. Corl ◽  
S.L. Hines ◽  
...  
Keyword(s):  
Drought Stress ◽  
Neutral Detergent Fiber ◽  
Fiber Digestibility

scholarly journals Characterization of a Drought-Inducible Dehydrin Promoter from Sugarcane (Saccharum officinarum L.) in Tobacco (Nicotiana tabacum L.)

2020 ◽  
Vol 7 (01) ◽  
pp. 28-36
Author(s):  
Hayati Minarsih Iskandar ◽  
Sonny Suhandono ◽  
Jembar Pambudi ◽  
Tati Kristianti ◽  
Riza Arief Putranto ◽  
...  
Keyword(s):  
Drought Stress ◽  
Tobacco Plant ◽  
Inducible Promoter ◽  
Saccharum Officinarum ◽  
Blue Color ◽  
Drought Treatment ◽  
Stress Treatment ◽  
Gus Reporter ◽  
Transformed Tobacco

Dehydrin (DHN) is known to play an important role in plant response and adaptation to abiotic stresses (drought, high salinity, cold, heat, etc.). Previous research reported the increased expression of DHN in sugarcane stems exposed to drought stress for 15 days which may be controlled by its corresponding stress inducible promoter. The DHN promoter was succesfully isolated from sugarcane variety PSJT 941 (Pr-1DHNSo) and was cloned to pBI121 expression vector fused to a β-glucuronidase (GUS) reporter gene.  The aim of this research was the functional testing of the Pr-1DHNSo promoter through transformation into tobacco plant treated with in vitro drought stress. Genetic transformation of Pr-1DHNSo construct was conducted by Agrobacterium tumefaciens. The transformed tobacco was then subjected to drought stress treatment using 40% PEG 6000  for five sequential incubations (0, 12, 24, 48 and 72 hours). The GUS assay reveal that the transformed tobacco treated with drought stress showed a blue color denoting GUS activity in leaf, stem and root tissues and this expression increased along with the length of the drought treatment. The analysis of gusA gene using real time-qPCR normalized to the L25 reference gene also showed that the expression increased in line with the length of time of drought stress.  The results presented in this study indicated that the Pr-1DHNSo promoter from sugarcane was expressed and induced by drought stress treatment in tobacco.

scholarly journals In vitro assessment of wheat tolerance to drought

Genetika ◽  
2005 ◽  
Vol 37 (2) ◽  
pp. 165-171 ◽  
Author(s):  
Vladislava Galovic ◽  
Zorana Kotaranin ◽  
Srbislav Dencic
Keyword(s):  
Drought Stress ◽  
Nutrient Medium ◽  
Callus Tissue ◽  
Geographic Origin ◽  
Control Group ◽  
Zygotic Embryos ◽  
Fresh Weight ◽  
Effects Of Drought ◽  
In Vitro Effects

Analyzed in this paper were the in vitro effects of drought stress in 13 genotypes of winter wheat, one genotype of spring wheat, and three Triticale genotypes of different geographic origin. Callus tissue was induced from immature zygotic embryos (10-15 days after pollination) on a modified MS nutrient medium. After two weeks, callus tissue was transplanted onto the same medium enriched with 5% high-molecular polyethylene glycol (PEG 6000), which was used as the stress agent to produce the effect of drought chemically. A control group of calluses was grown on an identical medium but without PEG. After four weeks of growing calluses on these mediums, we assessed callus mass survival ability of the genotypes before the transplantation as well as percentage reduction of callus fresh weight after the transplantation onto the nutrient medium with 5% PEG. Statistically significant differences were found among the genotypes in their response to the induced stress. The best survival ability before the transplantation was found in the genotype Mexicol20 (83%), while the lowest was recorded in Slavija (11.3%). Culture growing under stress conditions significantly reduced callus fresh weight in all of the genotypes. The lowest decrease of the callus mass relative to control was recorded in Rozofskaja (14.4%) and the highest in Miranovska (58.4%), indicating the genotypes' tolerance levels towards drought stress.

Identification of Ubiquitin Ligase From Grapevine Ring C3H2C3E3 and Characterization of Drought Resistance Function of VyRCHC114

2020 ◽  
Author(s):  
Yihe Yu ◽  
Shengdi Yang ◽  
Lu Bian ◽  
Keke Yu ◽  
Xiangxuan Meng ◽  
...  
Keyword(s):  
Drought Stress ◽  
Ubiquitin Ligase ◽  
Stress Responses ◽  
Phylogenetic Trees ◽  
Expression Profiles ◽  
E3 Ubiquitin Ligase ◽  
Pcr Analysis ◽  
Biotic And Abiotic Stress ◽  
Element Analysis

Abstract Background: RING is one of the largest E3 ubiquitin ligase families and C3H2C3 type is the largest subfamily of RING, playing an important role in plants’ development and growth and their biotic and abiotic stress responses. Results: A total of 143 RING C3H2C3-type genes (RCHCs) were discovered from the grapevine genome and separated into groups (I-XI) according to their phylogenetic analysis, with these genes named according to their positions on chromosomes. Gene replication analysis showed that tandem duplications play a predominant role in the expansion of VyRCHCs family together. Structural analysis showed that most VyRCHCs(67.13%) had no more than 2 introns, while genes clustered together based on phylogenetic trees had similar motifs and evolutionarily conserved structures. Cis-acting element analysis showed the diversity of VyRCHCs regulation. The expression profiles of eight DEGs in RNA-Seq after drought stress were similar to those in qRT-PCR analysis. The in vitro ubiquitin experiment showed that VyRCHC114 had E3 ubiquitin ligase activity, overexpression of VyRCHC114 in Arabidopsis improved drought tolerance, moreover, the transgenic plant survival rate increased by 30%, accompanied by changing of electrolyte leakage, chlorophyll content and the activities of SOD, POD, APX and CAT were changed. AtCOR15a, AtRD29A, AtERD15 and AtP5CS1 were expressed quantitatively, the results showed that they participated in the drought stress response may be regulated by the expression of VyRCHC114.Conclusions: Valuable new information on the evolution of grapevine RCHCs and its relevance for studying the functional characteristics of grapevine VyRCHC114 genes under drought stress emerged from this research.

scholarly journals Physiological Responses of Apple and Cherry In Vitro Culture under Different Levels of Drought Stress

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1689
Author(s):  
Zuzana Kovalikova ◽  
Petra Jiroutova ◽  
Jakub Toman ◽  
Dominika Dobrovolna ◽  
Lenka Drbohlavova
Keyword(s):  
Drought Stress ◽  
In Vitro Culture ◽  
Water Content ◽  
Membrane Damage ◽  
Dry Weight ◽  
Crop Productivity ◽  
Global Food Security ◽  
Fruit Species ◽  
Malondialdehyde Content

Drought stress is a serious threat. Therefore, improvements in crop productivity under conditions of limited water availability are vital to keep global food security. Apples and cherries belong to the most produced fruit worldwide. Thus, searching for their tolerant or resistant cultivars is beneficial for crop breeders to produce more resistant plants. We studied five apple (“Malinové holovouské”, “Fragrance”, “Rubinstep”, “Idared”, “Car Alexander”) and five cherry (“Regina”, “Napoleonova”, “Kaštánka”, “Sunburst”, “P-HL-C”) cultivars for their adaptation in response to progressive drought stress. The reaction of an in vitro culture to osmotic stress simulated by increasing polyethylene glycol (PEG) concentration in medium was evaluated through the morphological (fresh and dry weight, water content, leaf area), physiological (chlorophyll and carotenoids content), and biochemical (reactive oxygen species and malondialdehyde content) parameters. Drought-like stress negatively affected the water content, leaf areas, and chlorophyll content in both fruit species. Oxidative status and membrane damage of plants under water deficiency conditions occurred to be important indicators of stress tolerance mechanism. Cherries exhibited higher hydrogen peroxide levels compared to apples, whereas their malondialdehyde values were generally lower. The overall results indicated wide tolerance range to water deficit among apple and cherry in vitro culture as well as among cultivars within single plant species.

scholarly journals In vitro Selection of Drought Stress Tolerance in Rice (Oryza sativa L.) var. Jarava

2019 ◽  
Vol 29 (1) ◽  
pp. 127-131 ◽  
Author(s):  
Omar H Obaid ◽  
SK Reddy
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Plant Tissue ◽  
In Vitro Selection ◽  
Oryza Sativa L ◽  
Drought Stress Tolerance ◽  
Selection Of

Abstract not available Plant Tissue Cult. & Biotech. 29(1): 127-131, 2019 (June)

scholarly journals In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress

2020 ◽  
Vol 10 (13) ◽  
pp. 4471
Author(s):  
Didar Rahim ◽  
Petr Kalousek ◽  
Nawroz Tahir ◽  
Tomáš Vyhnánek ◽  
Petr Tarkowski ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Growth Parameters ◽  
Oryza Sativa L ◽  
Polymorphic Information Content ◽  
Callus Growth ◽  
Antioxidant Systems ◽  
In Vitro Tests ◽  
Rice Genotypes

Rice (Oryza sativa L.) is productively affected by different environmental factors, including biotic and abiotic stress. The objectives of this research were to evaluate the genetic distinction among Kurdish rice genotypes using the simple sequence repeats (SSRs) molecular markers and to perform in vitro tests to characterize the drought tolerance of six local rice genotypes. The polymorphic information content (PIC) varied from 0.38 to 0.84 with an average of 0.56. The genetic distance ranged from 0.33 to 0.88. Drought stress had a significant impact (p ≤ 0.05) on callus growth parameters. Enzymatic antioxidant systems were predicted and exhibited a significant variation. The findings revealed that proline levels increase in proportion to polyethylene glycol (PEG) concentrations. Kalar and Gwll Swr genotypes showed the worst performances in phenotypic and biochemical traits, while Choman and Shawre exhibited the best phenotypic and biochemical performances. A positive and substantial relationship between callus fresh weight (CFW) and callus dry weight (CDW) was found under stressful and optimized conditions. Callus induction (CI) was positively and significantly associated with the catalase activity (CAT) in all stressed treatments. Based on the results for callus growth and the biochemical parameters under stress conditions, a remarkable genotype distinction, based on the tolerance reaction, was noted as follows: PEG resistant > susceptible, Choman > Shawre > White Bazyan > Red Bazyan > Gwll Swr > Kalar. The CI and CAT characteristics were considered as reliable predictors of drought tolerance in rice genotypes.

scholarly journals In vitro screening of rice genotypes using polyethylene glycol under drought stress

2016 ◽  
Vol 27 (2) ◽  
pp. 128-135 ◽  
Author(s):  
J Akte ◽  
S Yasmin ◽  
MJH Bhuiyan ◽  
F Khatun ◽  
J Roy ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Water Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Shoot Length ◽  
Fresh Weight ◽  
Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

Ecophysiological, anatomical and biochemical aspects of in vitro culture of zygotic Syagrus coronata embryos and of young plants under drought stress

Trees ◽  
2015 ◽  
Vol 29 (4) ◽  
pp. 1219-1233 ◽  
Author(s):  
Maria Jaislanny Medeiros ◽  
Déborah Silva Oliveira ◽  
Marciel Teixeira Oliveira ◽  
Lilia Willadino ◽  
Laureen Houllou ◽  
...  
Keyword(s):  
Drought Stress ◽  
In Vitro Culture
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