scholarly journals In vitro pollen germination in avocado (Persea americana Mill.): Optimization of the method and effect of temperature

2011 ◽  
Vol 130 (1) ◽  
pp. 152-156 ◽  
Author(s):  
M.L. Alcaraz ◽  
M. Montserrat ◽  
J.I. Hormaza
Keyword(s):  
Pollen Germination ◽  
Persea Americana ◽  
Effect Of Temperature ◽  
In Vitro Pollen Germination

Effect of temperature on seed set and in vitro pollen germination in French beans (Phaseolus vulgaris)

1979 ◽  
Vol 19 (101) ◽  
pp. 725 ◽  
Author(s):  
PJ Farlow ◽  
DE Byth ◽  
NS Kruger
Keyword(s):  
Pollen Germination ◽  
Seed Set ◽  
Germination Percentage ◽  
French Bean ◽  
Hot Water ◽  
Effect Of Temperature ◽  
In Vitro Pollen Germination ◽  
French Beans ◽  
Different Temperatures

A technique for in vitro germination of French bean pollen was developed and the effect of temperature on gamete development, pollen germination and seed set investigated. Temperature had a profound effect on in vitro pollen germination percentage, pollen tube growth and bursting percentage. These were maximal at 7.2�C, 16.7�C and 38.3�C, respectively. In this study, pollen development was not affected at day/night temperatures of 16.1�/12.8�C, and ovule abortion was the cause of seed set failure at these temperatures. Hot water treatment (48�-44�C) of flowers caused failure of seed set due to pollen inviability. Consequently this technique may allow hybridization without emasculation in beans. Treatment of buds with hot water of different temperatures and in vitro pollen germination at high temperatures may have application as screening techniques for heat tolerance in French beans.

scholarly journals The effect of temperature on pollen germination and pollen tube growth of pear cultivars

2016 ◽  
Vol 61 (4) ◽  
pp. 333-341 ◽  
Author(s):  
Aleksandar Radovic ◽  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Dejan Djurovic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
Tube Length ◽  
In Vitro Pollen Germination ◽  
Different Temperatures ◽  
Pollen Tube Length

The effect of three different temperatures (8, 16 and 24?C) on pollen germination and length of pollen tube in vitro was investigated in four pear cultivars (?Butirra Precoce Morettini?, ?Williams?, ?Conference? and ?Abate Fetel?). The temperature showed a significant effect on in vitro pollen germination. The highest pollen germination was determined at a temperature of 24?C (53.25%), somewhat lower at 16?C (44.72%) and the lowest at 8?C (23.16%). The temperature effect was significantly more pronounced on the length of pollen tube. Pollen tube length was about three times higher at the temperatures of 16 and 24?C compared to 8?C. The temperature of 8?C was not sufficient for pollen germination and pollen tube growth in pear cultivars. However, temperatures of 16 and 24?C were optimal for pollen germination and pollen tube growth.

scholarly journals The effect of pollenizer on the fruit set of plum cultivar Cacanska Najbolja

2012 ◽  
Vol 57 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Dragan Nikolic ◽  
Vera Rakonjac ◽  
Milica Fotiric-Aksic
Keyword(s):  
Pollen Germination ◽  
Fruit Set ◽  
Open Pollination ◽  
In Vitro Pollen Germination ◽  
Controlled Pollination ◽  
Very High

In this paper, during a 4-year period (2003-2006) effects of six pollenizers (President, Italian Prune, Stanley, Cacanska Rodna, Agen 707, and California Blue) on the degree of fruit set in the plum cultivar Cacanska Najbolja were examined. Besides the controlled pollination of this cultivar, open pollination was investigated. Functional pollen ability in pollenizer-cultivars was established by in vitro pollen germination. Degree of fruit set was determined comparing the number of fruit set (10 days after pollination, 21 days after pollination and number of harvested fruits) against the number of pollinated flowers. The results indicated that all pollenizer cultivars, studied in this paper, possessed satisfactory in vitro pollen germination (30.1-67.4%). The number of fruit set determined 10 days after pollination was very high and did not differ among pollenizers. Highly significant differences were found between the pollenizers in the number of fruit set 21 days after pollination and significant ones in relation to the number of harvested fruits. Compared to open pollination, higher number of fruit set 21 days after pollination and higher number of harvested fruits were obtained when cultivars Stanley (17.0%; 7.6%) and Italian Prune (14.6%; 6.9%) were used as pollenizers, therefore those cultivars are recommendable as good pollenizers for the cultivar Cacanska Najbolja.

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