High frequency in vitro embryogenic callus induction and plant regeneration from indiangrass mature caryposis

2009 ◽  
Vol 119 (3) ◽  
pp. 306-309 ◽  
Author(s):  
Yonghong Li ◽  
Junping Gao ◽  
Shui-zhang Fei
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
High Frequency ◽  
Embryogenic Callus ◽  
Embryogenic Callus Induction

scholarly journals Potential Embryogenic Callus Induction Protocol Through Cell Suspension Culture For High Frequency Plant Regeneration Of Maspine Pineapple (Ananas comosus L.)

1970 ◽  
Vol 3 (2) ◽  
pp. 40-45
Author(s):  
M.F. Mohamad Bukhori ◽  
Norzulaani Khalid ◽  
Ch'ng Lou Ven
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Embryogenic Callus Induction

To explore the potential for embryogenic callus induction protocol through cell suspension culture forhigh frequency plant regeneration of Maspine pineapple (Ananas comosus L.), eight different culturemedia formulation were evaluated for their effects on the induction of somatic embryos from suckerexplants. Explants were cultured on MS medium supplemented with various media concentration(NAA, Dicamba and BAP, Picloram, Kinetin and NAA, 2,4-D, TDZ, and TDZ and BAP).Embryogenic callus induction percentage, color and texture of the callus were assessed after fivemonths of culture. The optimum medium for the proliferation of in vitro shoots from sucker explantswas MS medium supplemented with 3 mg/L BAP. Meanwhile, the optimum medium for the inductionof fastest and high percentage of embryogenic callus growth from in vitro leaf-based was MS mediumsupplemented with Picloram. Results of mean comparison showed that 3 mg/L Picloram were moreeffective on explants than 10 mg/L. Results of the double staining method proved that somaticembryogenesis occurred in MS supplemented with 3 mg/L Picloram. Under microscopic observations,the globular-stage of the embryos were revealed in callus cells which is relatively suitable forsuspension cells inoculums, indicating that the tested PGR were significantly effective for somaticembryogenesis formation in this species. Most embryogenic callus from sucker explants wasyellowish-mucilaginous-wet-friable. The developed protocol potentially leads to the production ofembryogenic callus from sucker explants and plant regeneration through somatic embryogenesis.

scholarly journals Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum)

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Shakra Jamil ◽  
Rahil Shahzad ◽  
Ghulam Mohyuddin Talha ◽  
Ghazala Sakhawat ◽  
Sajid-ur-Rahman ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Shoot Elongation ◽  
Root Induction ◽  
Rooting Media ◽  
Embryogenic Callus Formation

Sugarcane contributes 60–70% of annual sugar production in the world. Somaclonal variation has potential to enhance genetic variation present within a species. Present study was done to optimize an in vitro propagation protocol for sugarcane. The experiments included four varieties, 9 callus induction media, 27 regeneration media, and 9 root induction media under two-factor factorial CRD. Data were recorded on callus induction, embryogenic callus formation, shoot elongation (cm), root induction, and plant regeneration. Statistically significant differences existed between genotypes and treatments for callus induction (%), embryogenic callus formation (%), shoot elongation (cm), root induction, and plant regeneration (%). All parameters showed dependency on genotypes, culture media, and their interaction. Highest callus induction (95%) embryogenic callus formation (95%) was observed in callus induction media 5. Highest plantlet regeneration (98.9%) capacity was observed in regeneration media 11 whereas maximum shoot elongation (12.13 cm) and root induction (8.32) were observed in rooting media 4. G1 showed best response for all traits and vice versa for G4. Hence it was concluded that G1, callus induction media 5, regeneration media 11, and rooting media 4 are the best conditions for in vitro propagation of sugarcane.

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