scholarly journals In vitro germination and seedling development of cryopreserved Dendrobium hybrid mature seeds

2007 ◽  
Vol 114 (3) ◽  
pp. 188-193 ◽  
Author(s):  
Wagner A. Vendrame ◽  
V.S. Carvalho ◽  
J.M.M. Dias
Keyword(s):  
Seedling Development ◽  
In Vitro Germination ◽  
Mature Seeds

Germination of cut seeds and seedling growth of ash (Fraxinus spp.) in vitro

1995 ◽  
Vol 25 (8) ◽  
pp. 1368-1374 ◽  
Author(s):  
John E. Preece ◽  
Sharon A. Bates ◽  
J.W. Van Sambeek
Keyword(s):  
Plant Growth ◽  
Growth Regulators ◽  
Seedling Development ◽  
Green Ash ◽  
White Ash ◽  
Murashige And Skoog Medium ◽  
Epicotyl Elongation ◽  
Viable Seeds ◽  
Mature Seeds

Nonstratified seeds of different genotypes of Fraxinusamericana L. (white ash), Fraxinuspennsylvanica Marsh, (green ash), and Fraxinusangustifolia Vahl. ssp. oxycarpa (narrowleaf ash, of Afghanistan origin) were surface disinfested, cut, and germinated in vitro on agar-solidified MS (Murashige and Skoog) medium containing sucrose and various plant growth regulators. Before placing in vitro, approximately one-third of the seed opposite the radicular end was excised and discarded, or the apical and basal 1 mm tips of the seed were aseptically excised. Within 1 week, viable seeds germinated. Cotyledons emerged, elongated, expanded, and turned green. After 2 weeks hypocotyls and radicles elongated. White and green ash genotypes germinated at different rates; generally >80% of seeds placed in vitro germinated. Genotype and the presence of thidiazuron in the medium had an effect on seedling development (hypocotyl and epicotyl elongation). Immature as well as nonstratified mature seeds germinated and grew. Epicotyls were excised, rooted, and established in the greenhouse.

scholarly journals Phenology and seed germination of the Indian Screw Tree Helicteres isora L. (Malvales: Malvaceae)

2017 ◽  
Vol 9 (12) ◽  
pp. 11040 ◽  
Author(s):  
Mariappan Muthukumar ◽  
Thiruppathi Senthil Kumar ◽  
Mandali Venkateswara Rao
Keyword(s):  
Seed Viability ◽  
Early Summer ◽  
Seedling Development ◽  
Ms Medium ◽  
Seed Setting ◽  
Flower Buds ◽  
Helicteres Isora ◽  
Half Strength ◽  
Mature Seeds

Helicteres isora L. (Malvaceae), a medicinally important tree species used in Ayurveda as well as by folklore practitioners, is studied for its phenology, associated problems with the environment and anthropogenic effects.  In Helicteres isora, flower buds are initiated by the end of July and flowering occurs during the middle of August to late October.  Fruits mature during late January to early April.  In between various factors like blister beetles, unidentified worms, deficit rainfall, early summer affect the flowering and seed setting, which cause 35–45 % loss.  Seed viability of mature seeds were recorded 88.2% at fresh collection and decreases over period of time. In vitro, among the various treatments, acid treated seeds (H2SO4, 98.0%; 1 min) resulted in the best germination response of 84.5%.  Sterile wet cotton soaked with sterile distilled water was found suitable for germination compared to other substratum, while half-strength MS medium with 2.0% sucrose was suitable for seedling development.

scholarly journals In Vitro Germination and Initial Seedling Development of Krantz Aloe by Smoke-saturated Water and Seed Imbibition

2020 ◽  
Vol 30 (5) ◽  
pp. 619-623
Author(s):  
Claudia A. Espinosa-Leal ◽  
Silverio Garcia-Lara
Keyword(s):  
Response Times ◽  
Culture Media ◽  
Seedling Development ◽  
In Vitro Germination ◽  
Wild Populations ◽  
Active Ingredients ◽  
Seed Imbibition ◽  
Saturated Water ◽  
Pharmaceutical Industries

Krantz aloe (Aloe arborescens) is one of two aloe species currently used for the extraction of active ingredients that can be useful in the cosmetic and pharmaceutical industries. However, krantz aloe plants have been frequently collected from the wild, which is threatening wild populations. In vitro tissue culture would allow the growth of krantz aloe under controlled conditions, optimizing the production of active ingredients without risk to wild populations. The establishment of cultures from krantz aloe plant explants has proved difficult as a result of the long response times of the explants and their release of polyphenols. Krantz aloe seeds are not commonly used as a means of propagation because of their low germination percentages. The objective of this study was to evaluate the effects of seed imbibition (SI) with water and the addition of smoke-saturated water (SSW) to the culture medium on the in vitro germination and initial seedling development of krantz aloe. Seeds were germinated in vitro in axenic conditions. The treatments used were the addition of 10% SSW to the culture media, SI, and a combination of both (10% SSW + SI). Daily germination was recorded and gross morphology was measured after 1 month of culture establishment. The maximum germination percentage (GP) was achieved when 10% SSW was in the medium (97.2%), followed by the combined application of 10% SSW + SI (96.6%), both of which were almost 30% greater and significantly different from that of the control (69.4%). SI had an 83.3% GP. No significant differences were observed among treatments in any of the observed development parameters (leaf and root length and number). Taken together, these findings show that the use of SSW improves the in vitro germination of krantz aloe without affecting seedling development, indicating preliminarily that SSW represents a useful in vitro germination promoter for this species.

scholarly journals In Vitro Culture of Zygotic Embryos of Taxus Species

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 695-697 ◽  
Author(s):  
Paula P. Chee
Keyword(s):  
In Vitro Culture ◽  
Embryo Culture ◽  
Culture Method ◽  
Seedling Development ◽  
Zygotic Embryos ◽  
Salt Medium ◽  
Basal Salt Medium ◽  
Radicle Emergence ◽  
Mature Seeds

An embryo culture method overcomes the lengthy dormancy requirement of Taxus L. spp. (yew) seeds. When zygotic embryos excised from mature T. brevifolia L. seeds were cultured in darkness for 4 weeks on one of three basal salt media (B5, Litvay, and Murashige and Skoog), radicle emergence and seedling development was highest on B5 basal salt medium. After 1 to 2 weeks on B5 basal salt medium, seedling development of T. brevifolia, T. cuspidata L., T. baccata L., and T. baccata stricta L. ranged from 2% to 36%. BA at 2.25 μm had no effect on radicle emergence; 22.5 μm prevented it. Embryos excised from mature or nearly mature seeds had the highest frequency of radicle emergence and seedling development. Cultured embryos developed seedlings in only 8 to 10 weeks. Chemical name used: N6-benzyladenine (BA).

In vitro germination and seedling development of Posidonia oceanica

1998 ◽  
Vol 60 (1) ◽  
pp. 83-93 ◽  
Author(s):  
Elena Balestri ◽  
Luigi Piazzi ◽  
Francesco Cinelli
Keyword(s):  
Posidonia Oceanica ◽  
Seedling Development ◽  
In Vitro Germination
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