Effect of 2,4-d, glutamine and BAP on embryogenic suspension culture of date palm (Phoenix dactylifera L.)

2007 ◽  
Vol 112 (2) ◽  
pp. 221-226 ◽  
Author(s):  
Jamila Zouine ◽  
Ismaïl El Hadrami
2018 ◽  
Vol 42 (5) ◽  
pp. 464-473 ◽  
Author(s):  
Poornananda Madhava Naik ◽  
Jameel Mohammed Al-Khayri

ABSTRACT Date palm accumulates a wide range of secondary metabolites high in nutritional and therapeutic value. In the present study, date palm (Phoenix dactylifera L., cv. Shaishi) shoot-tip-induced callus was used to establish cell suspension cultures in Murashige and Skoog (MS) liquid medium containing 1.5 mg L-1 2-isopentenyladenine (2iP) and 10 mg L-1 naphthaleneacetic acid (NAA). To study the growth kinetics, cultures were maintained for 12 weeks during which weekly measurements were carried out to determine the biomass accumulation based on packed cell volume (%), fresh weight and dry weight (g). In addition, weekly determination of polyphenols (catechin, caffeic acid, kaempferol, and apigenin) was carried out using high performance liquid chromatography (HPLC). The 11-week-old culture was found highest in the production of biomass (62.9 g L-1 fresh weight and 7.6 g L-1 dry weight) and polyphenols (catechin-155.9 µg L-1, caffeic acid-162.7 µg L-1, kaempferol-89.7 µg L-1, and apigenin-242.7 µg L-1) from the cell suspension cultures. This is the first report on the production of polyphenols from the cell suspension culture of date palm. This study facilitates further development of large-scale production of polyphenols and the utilization of bioreactors.


1993 ◽  
Vol 8 (1-4) ◽  
pp. 156-162 ◽  
Author(s):  
Jens I. Find ◽  
Franz Floto ◽  
Peter Krogstrup ◽  
Jette Dahl M⊘ller ◽  
Jens V. N⊘rgaard ◽  
...  

LWT ◽  
2021 ◽  
pp. 111762
Author(s):  
Ibrahim A. Almusallam ◽  
Isam A. Mohamed Ahmed ◽  
Elfadil E. Babiker ◽  
Fahad Y. Al-Juhaimi ◽  
Ali Saleh ◽  
...  

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