Screening Capsicum species of different origins for high temperature tolerance by in vitro pollen germination and pollen tube length

2007 ◽  
Vol 112 (2) ◽  
pp. 130-135 ◽  
Author(s):  
K. Raja Reddy ◽  
V.G. Kakani
COCOS ◽  
2012 ◽  
Vol 19 (1) ◽  
pp. 13
Author(s):  
C S Ranasinghe ◽  
K P Waidyarathna ◽  
A P C Pradeep ◽  
M S K Meneripitiya

Genetika ◽  
2017 ◽  
Vol 49 (3) ◽  
pp. 791-800
Author(s):  
Milica Fotiric-Aksic ◽  
Radosav Cerovic ◽  
Vera Rakonjac ◽  
Ivana Bakic ◽  
Slavica Colic ◽  
...  

Vitality of pollen, in vitro pollen germination and pollen tube growth (pollen tube length and pollen tube growth rate) were investigated in Oblacinska sour cherry in order to determine the differences between clones which have divergent yielding potential. For this purpose two ?Oblacinska? sour cherry clones with high fruit set and high yields (II/2, III/9) and two with low fruit set and low-yielding (XI/3 and XIII/1) were used in this study. Pollen germination was done on artificial medium containing 14% sucrose and 0.3% agar-agar at room temperature (23?C). Pollen tube growth was stopped with a drop of 40% formaldehyde, 1, 3, 6, 12 and 24 h after contact with the medium. The maximum percentage of germination ranged from 13.01% (clone II/2, after 1 h) to 54.19% (clone III/9, after 24 h). Pollen tube length varied from 64.84 ?m (clone XIII/1, after 1 h) to >1,100 ?m (clones II/2 and III/9, after 24 h). Pollen growth rate was quite high (up to 1.71 ?m min-1) after 6 h of germination, but rather decreasing until 24 h of germination (0.56-0.83 ?m min-1). The dynamics of in vitro pollen tubes growth among the clones were quite different, especially after 12 h and 24 h of germination. Clones that are singled out as fruitful (II/2 and III/9) gave much better results regarding pollen germination and pollen tube growth in comparison to clones which were characterized by low fruit set and yields (XI/3 and XIII/1).


2016 ◽  
Vol 61 (4) ◽  
pp. 333-341 ◽  
Author(s):  
Aleksandar Radovic ◽  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Dejan Djurovic

The effect of three different temperatures (8, 16 and 24?C) on pollen germination and length of pollen tube in vitro was investigated in four pear cultivars (?Butirra Precoce Morettini?, ?Williams?, ?Conference? and ?Abate Fetel?). The temperature showed a significant effect on in vitro pollen germination. The highest pollen germination was determined at a temperature of 24?C (53.25%), somewhat lower at 16?C (44.72%) and the lowest at 8?C (23.16%). The temperature effect was significantly more pronounced on the length of pollen tube. Pollen tube length was about three times higher at the temperatures of 16 and 24?C compared to 8?C. The temperature of 8?C was not sufficient for pollen germination and pollen tube growth in pear cultivars. However, temperatures of 16 and 24?C were optimal for pollen germination and pollen tube growth.


2017 ◽  
Vol 54 (5) ◽  
pp. 731-743 ◽  
Author(s):  
C. S. RANASINGHE ◽  
M. D. P. KUMARATHUNGE ◽  
K. G. S. KIRIWANDENIYA

SUMMARYSuccessful fruit set in coconut depends on several reproductive processes including pollen germination and pollen tube growth. High temperature (˃33 °C) during flowering reduces fruit set in coconut. Therefore, identification and development of coconut varieties or hybrids with high reproductive heat tolerance will benefit the coconut industry in view of the climate changes. This experiment was conducted to quantify the response of pollen germination and pollen tube growth of seven coconut hybrids to increasing temperature from 16 to 38 °C. A Principal Component Analysis (PCA) was carried out to classify coconut hybrids on the basis of their temperature tolerances to pollen germination. Pollen germination and pollen tube length of the hybrids ranged from 56 to 78% and 242 to 772 µm, respectively. A modified bilinear model best described the response to temperature of pollen germination and pollen tube length. Cardinal temperatures (Tmin, Topt and Tmax) of pollen germination and pollen tube length varied among the seven hybrids. PCA identified Tmax for pollen germination and Topt for pollen tube length as the most important parameters in describing varietal tolerance to high temperature. PCA also identified SLGD × Sri Lanka Tall and Sri Lanka Brown Dwarf × Sri Lanka Tall as the most tolerant hybrids to high temperature stress and Sri Lanka Tall × Sri Lanka Tall and Sri Lanka Green Dwarf × San Ramon as less tolerant ones based on cardinal temperatures for pollen germination and pollen tube length. Tmax for pollen germination of the most tolerant and less tolerant hybrids were 41.9 and 39.5 °C, respectively. Topt for pollen tube length in the most tolerant and less tolerant hybrids were 29.5 and 26.0 °C, respectively.


2005 ◽  
Vol 96 (1) ◽  
pp. 59-67 ◽  
Author(s):  
V. G. KAKANI ◽  
K. R. REDDY ◽  
S. KOTI ◽  
T. P. WALLACE ◽  
P. V. V. PRASAD ◽  
...  

2020 ◽  
Vol 15 (2) ◽  
pp. 183-190
Author(s):  
M Ganga ◽  
J Lakshmi ◽  
N Manivannan ◽  
K Rajamani

The present investigation was carried out at the Department of Floriculture and Landscape Architecture, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore during 2017-2019. The study involved nine jasmine genotypes, four falling under the commercially cultivated types and five belonging to underutilized species or ‘lesser-known species’. The study was undertaken to investigate and document the palynological parameters of jasmines which could serve as a reliable reference for future jasmine breeding programmes. The palynological investigations were carried out using Scanning Electron Microscopy (SEM), haemocytometry, acetocarmine test, and in vitro pollen germination. The pollen morphology analysis indicated wide variation among the species for the shape of the pollen grain, ranging from tricolpate to prolate; the exine ornamentation was reticulate in all the genotypes. Pollen output was the highest in J. rigidum (28,660 pollen/flower) and the lowest in (625 pollen/ flower) in J. sambac cv. Ramanathapuram Gundumalli. The maximum pollen germination rate and pollen tube length were recorded in J. rigidum.


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