In vitro regeneration and genetic transformation of common bean (Phaseolus vulgaris L.)—Problems and progress

2005 ◽  
Vol 107 (1) ◽  
pp. 2-10 ◽  
Author(s):  
M. Veltcheva ◽  
D. Svetleva ◽  
Sp. Petkova ◽  
A. Perl
Keyword(s):  
Phaseolus Vulgaris ◽  
Genetic Transformation ◽  
Common Bean ◽  
In Vitro Regeneration ◽  
Phaseolus Vulgaris L

scholarly journals Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca

2019 ◽  
Vol 67 (2SUPL) ◽  
pp. S83-S94 ◽  
Author(s):  
Laura-Yesenia Solís-Ramos ◽  
Julio-César Ortiz-Pavón ◽  
Antonio Andrade-Torres ◽  
Romano Porras-Murillo ◽  
Arturo Brenes-Angulo ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Phaseolus Vulgaris ◽  
Genetic Transformation ◽  
Common Bean ◽  
In Vitro Regeneration ◽  
Marker Genes ◽  
Transformation Protocol ◽  
Glucuronidase Activity ◽  
Rich Media

Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene.

Effect of the Addition of Common Bean (Phaseolus vulgaris L.) Flour on the In Vitro Digestibility of Starch and Undigestible Carbohydrates in Spaghetti

2010 ◽  
pp. no-no ◽  
Author(s):  
Jose-Alberto Gallegos-Infante ◽  
Luis Arturo Bello-Perez ◽  
Nuria Elizabeth Rocha-Guzman ◽  
Ruben Francisco Gonzalez-Laredo ◽  
Martha Avila-Ontiveros
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
In Vitro Digestibility ◽  
Phaseolus Vulgaris L
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