Genetic diversity in yardlong bean (Vigna unguiculata ssp. sesquipedalis) and related Vigna species using sequence tagged microsatellite site analysis

2005 ◽  
Vol 106 (2) ◽  
pp. 137-146 ◽  
Author(s):  
P. Phansak ◽  
P.W.J. Taylor ◽  
O. Mongkolporn
Keyword(s):  
Genetic Diversity ◽  
Vigna Unguiculata ◽  
Site Analysis ◽  
Vigna Species ◽  
Yardlong Bean

scholarly journals Analysis of Yardlong Bean (Vigna unguiculata Subsp. sesquipedalis) Genetic Diversity using RAPD Markers

2020 ◽  
Vol 12 (2) ◽  
pp. 127-135
Author(s):  
Muhammad Shahidul Haque ◽  
Afifa Azad ◽  
Nihar Ranjan Saha ◽  
Muhammad Monirul Islam
Keyword(s):  
Genetic Diversity ◽  
Vigna Unguiculata ◽  
Rapd Markers ◽  
Yardlong Bean

Assessment of genetic diversity in yardlong bean (Vigna unguiculata (L.) Walp subsp. sesquipedalis Verdc.) germplasm from India using RAPD markers

2019 ◽  
Vol 66 (6) ◽  
pp. 1231-1242
Author(s):  
Saidaiah Pidigam ◽  
Suchandranath Babu Munnam ◽  
Srinivas Nimmarajula ◽  
Narshimulu Gonela ◽  
Srivani S. Adimulam ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vigna Unguiculata ◽  
Rapd Markers ◽  
Yardlong Bean

scholarly journals Multivariate analysis unravels genetic diversity and relationship between agronomic traits, protein, and dietary fiber in yardlong bean (Vigna unguiculata subsp. sesquipedalis Verdc.)

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Muhammad Habib Widyawan ◽  
Aulia Hasanah ◽  
Taryono ◽  
Taufan Alam ◽  
Rahmi Sri Sayekti ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Multivariate Analysis ◽  
Dietary Fiber ◽  
Vigna Unguiculata ◽  
Agronomic Traits ◽  
High Yield ◽  
Nutritional Content ◽  
Total Dietary Fiber ◽  
Yardlong Bean ◽  
Positive Direct

Abstract. Widyawan MB, Hasanah A, Taryono, Alam T, Sayekti RS, Pramana AAC, Wulandari RA. 2020. Multivariate analysis unravels genetic diversity and relationship between agronomic traits, protein, and dietary fiber in yardlong bean (Vigna unguiculata subsp. sesquipedalis Verdc.). Biodiversitas 21: 5662-5671. Yardlong bean (Vigna unguiculata subsp. sesquipedalis Verdc.) is a vegetable legume that possesses the highest amount of protein and total dietary fiber (TDF) content among other vegetables. However, protein and TDF content diversity are found among yardlong bean genotypes. Genetic improvement to produce cultivars with high yield and improved nutritional content is required. This study aimed to analyze genetic diversity and identify the relationship between important agronomic traits from 21 yardlong bean genotypes assessed by multivariate statistical analysis. The experiment was arranged in alpha design with three replicates, each consist of six blocks. Six pod-related important agronomic traits, protein, and TDF content were observed in this study. Total pod weight, pod weight, and pod length were found to be negatively correlated with protein and TDF content based on correlation and principal component analysis (PCA). Although negatively correlated, several variables possess a large and positive direct effect on protein and TDF content, such as the number of pod, pod weight, and days to flower. D2 analysis and Tocher’s method classify genotypes used in this study into five clusters with diverse traits value. Information from this study laid a foundation for the improvement of important agronomic traits as well as nutritional content in yardlong bean.

An SSR-based linkage map of yardlong bean (Vigna unguiculata (L.) Walp. subsp. unguiculata Sesquipedalis Group) and QTL analysis of pod length

Genome ◽  
2012 ◽  
Vol 55 (2) ◽  
pp. 81-92 ◽  
Author(s):  
Alisa Kongjaimun ◽  
Akito Kaga ◽  
Norihiko Tomooka ◽  
Prakit Somta ◽  
Takehiko Shimizu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Segregation Distortion ◽  
Vigna Unguiculata ◽  
Length Variation ◽  
Molecular Linkage Map ◽  
Vigna Species ◽  
Vigna Umbellata ◽  
Yardlong Bean ◽  
Pod Length ◽  
Simple Sequence

Yardlong bean (Vigna unguiculata (L.) Walp. subsp. unguiculata Sesquipedalis Group) (2n = 2x = 22) is one of the most important vegetable legumes of Asia. The objectives of this study were to develop a genetic linkage map of yardlong bean using SSR makers from related Vigna species and to identify QTLs for pod length. The map was constructed from 226 simple sequence repeat (SSR) markers from cowpea (Vigna unguiculata (L.) Walp. subsp. unguiculata Unguiculata Group), azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi), and mungbean (Vigna radiata (L.) Wilczek) in a BC1F1 ((JP81610 × TVnu457) × JP81610) population derived from the cross between yardlong bean accession JP81610 and wild cowpea (Vigna unguiculata subsp. unguiculata var. spontanea) accession TVnu457. The markers were clustered into 11 linkage groups (LGs) spanning 852.4 cM in total length with a mean distance between adjacent markers of 3.96 cM. All markers on LG11 showed segregation distortion towards the homozygous yardlong bean JP81610 genotype. The markers on LG11 were also distorted in the rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) map, suggesting the presence of common segregation distortion factors in Vigna species on this LG. One major and six minor QTLs were identified for pod length variation between yardlong bean and wild cowpea. Using flanking markers, six of the seven QTLs were confirmed in an F2 population of JP81610 × TVnu457. The molecular linkage map developed and markers linked to pod length QTLs would be potentially useful for yardlong bean and cowpea breeding.

scholarly journals Genetic diversity analysis of yardlong bean genotypes (Vigna unguiculata subsp. sesquipedalis) based on IRAP marker

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Muhammad Habib Widyawan ◽  
Sri Wulandary ◽  
Taryono
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Vigna Unguiculata ◽  
Genetic Similarity ◽  
Polymorphic Information Content ◽  
Resolving Power ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Irap Marker ◽  
Yardlong Bean

Abstract. Widyawan MH, Wulandary S, Taryono. 2020. Genetic diversity analysis of yardlong bean genotypes (Vigna unguiculata subsp. sesquipedalis) based on IRAP marker. Biodiversitas 21: 1101-1107. Inter-Retrotransposon Amplified Polymorphism (IRAP) marker is a PCR-based molecular marker that detects polymorphism between retrotransposon sites. This marker has been utilized and successfully assessed genetic diversity in many crop species. Yardlong bean (Vigna unguiculata subsp. sesquipedalis) was an important vegetable legume crop that grown mainly for its fresh pod that rich in nutritional benefits for humans, ultimately dietary fiber and protein. Trends of people awareness to nutritional content of food are increasing, therefore breeding for quality traits is important. Genetic diversity analysis is an important and elementary step in breeding programs in order to determine the breeding strategy. The aims of this research are to perform an optimization of IRAP marker and applied it for genetic diversity analysis in 16 yardlong bean genotypes. Seven primers were used as marker in a pair or single combination and resulted in 11 optimized markers that able to be used for genetic diversity analysis. Sixteen yardlong bean genotypes consisting of commercial cultivars and local genotypes from Indonesia were genotyped using eleven IRAP markers. Marker polymorphism and diversity parameters from each marker i.e. Percentage of Polymorphic Loci (PPL), Expected Heterozygosity (He), Polymorphic Information Content (PIC), Effective Multiplex Ratio (EMR), Marker Index (MI), Discriminating Power (D), and Resolving Power (RP) were calculated. Based on those values, several markers used in this study were considered as informative and efficient in terms of analyzing genetic diversity in yardlong bean. Jaccard's method was used to measure genetic similarity and it is revealed that there is high level of similarity between yardlong bean genotypes used in this study. Thus, it is implied that there is a narrow genetic diversity of yardlong bean genotypes used in this study. Cluster analysis was performed to construct dendrogram based on genetic similarity and classified 16 yardlong genotypes into 4 clusters. Interestingly, majority of the clusters formed were not able to classified genotypes based on their origin. The result of cluster analysis then confirmed by Principal Coordinate Analysis (PCoA) that able to explain 47.76 % of total variation. The results of this study provide a foundation for the genetic diversity analysis based on IRAP marker and genetic improvement in yardlong bean.

scholarly journals Genetic diversity and population structure of a mini-core subset from the world cowpea (Vigna unguiculata (L.) Walp.) germplasm collection

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Christian Fatokun ◽  
Gezahegn Girma ◽  
Michael Abberton ◽  
Melaku Gedil ◽  
Nnanna Unachukwu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Vigna Unguiculata ◽  
Germplasm Collection ◽  
Core Subset ◽  
The World

Evaluation and QTL Mapping of Salt Tolerance in Yardlong Bean [Vigna unguiculata (L.) Walp. Subsp. unguiculata Sesquipedalis Group] Seedlings

2020 ◽  
Vol 38 (2) ◽  
pp. 294-304
Author(s):  
Hongmei Zhang ◽  
Wenjing Xu ◽  
Huatao Chen ◽  
Jingbin Chen ◽  
Xin Chen ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Qtl Mapping ◽  
Vigna Unguiculata ◽  
Yardlong Bean
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