Using ATR-FT/IR molecular spectroscopy to detect effects of blend DDGS inclusion level on the molecular structure spectral and metabolic characteristics of the proteins in hulless barley

2012 ◽  
Vol 95 ◽  
pp. 53-63 ◽  
Author(s):  
Xuewei Zhang ◽  
Peiqiang Yu
Keyword(s):  
Molecular Structure ◽  
Molecular Spectroscopy ◽  
Hulless Barley ◽  
Metabolic Characteristics ◽  
Ft Ir ◽  
Inclusion Level

scholarly journals Interactive association between processing induced molecular structure changes and nutrient delivery on a molecular basis, revealed by cutting-edge vibrational biomolecular spectroscopy

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Aya Ismael ◽  
Victor Hugo Guevara-Oquendo ◽  
Basim Refat ◽  
Peiqiang Yu
Keyword(s):  
Molecular Structure ◽  
Molecular Spectroscopy ◽  
Degradation Kinetics ◽  
Structural Features ◽  
Molecular Structures ◽  
Non Invasive ◽  
Structure Changes ◽  
Ft Ir ◽  
Α Helix ◽  
Β Sheet

Abstract Background This study was conducted to determine protein molecular structure profiles and quantify the relationship between protein structural features and protein metabolism and bioavailability of blend pelleted products (BPP) based on co-products (canola or carinata) from processing with different proportions of pulse pea screenings and lignosulfonate chemical compound. Method The protein molecular structures were determined using the non-invasive advanced vibrational molecular spectroscopy (ATR-FT/IR) in terms of chemical structure and biofunctional groups of amides (I and II), α-helix and β-sheet. Results The results showed that increasing the level of the co-products in BPP significantly increased the spectral intensity of the amide area and amide height. The products exhibited similar protein secondary α-helix to β-sheet ratio. The protein molecular structure profiles (amides I and II, α-helix to β-sheet) were highly associated with protein degradation kinetics and intestinal digestion. In conclusion, the non-invasive vibrational molecular spectroscopy (ATR-FT/IR) could be used to detect inherent structural make-up characteristics in BPP. Conclusion The molecular structural features related to protein biopolymer were highly associated with protein utilization and metabolism.

Molecular structure analysis of ascending aorta aneurysm upon atherosclerosis

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
I Mamarelis ◽  
V Mamareli ◽  
M Kyriakidou ◽  
O Tanis ◽  
C Mamareli ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Raman Spectra ◽  
Molecular Level ◽  
Ascending Aorta ◽  
Ir And Raman Spectra ◽  
Aggregate Formation ◽  
Aorta Aneurysm ◽  
Ft Ir ◽  
Molecular Structure Analysis ◽  
Ir And Raman

Abstract Background The atherosclerotic ascending aorta could represent a potential source of emboli or could be an indicator of atherosclerosis in general with high mortality. The mechanism of aneurysm formation and atherosclerosis of the ascending aorta at the molecular level has not yet been clarified. To approach the mechanism of ascending aortic lesions and mineralization at a molecular level, we used the non-destructive FT-IR, Raman spectroscopy, SEM and Hypermicroscope. Methods Six ascending aorta biopsies were obtained from patients who underwent aortic valve replacement (AVR) cardiac surgery. CytoViva (einst inc) hyperspectral microscope was used to obtain the images of ascending aorta. The samples were dissolved in hexane on a microscope glass plate. The FT-IR and Raman spectra were recorded with Nicolet 6700 thermoshintific and micro-Raman Reinshaw (785nm, 145 mwatt), respectively. The architecture of ascending aorta biopsies was obtained by using scanning electron microscope (SEM of Fei Co) without any coating. Results FT-IR and Raman spectra showed changes arising from the increasing of lipophilic environment and aggregate formation (Fig. 1). The band at 1744 cm–1 is attributed to aldehyde CHO mode due to oxidation of lipids. The shifts of the bands of the amide I and amide II bands to lower are associated with protein damage, in agreement with SEM data. The bands at about 1170–1000 cm–1 resulted from the C-O-C of advanced glycation products as result of connecting tissues fragmentations and polymerization. The spectroscopic data were analogous with the lesions observed with SEM and hypermicroscopic images. Conclusions The present innovate molecular structure analysis showed that upon ascending aorta aneurysm development an excess of lipophilic aggregate formation and protein lesions, changing the elasticity of the aorta's wall. The released Ca2+ interacted mostly with carbonate-terminal of cellular protein chains accelerated the ascending aorta calcifications. Figure 1. FT-IR and Raman spectra Funding Acknowledgement Type of funding source: None

Molecular structure of 1-(2'-hydroxyaryl)-hydantoines; an assessment by 13C NMR, FT IR and semi-empirical calculations

1998 ◽  
Vol 444 (1-3) ◽  
pp. 63-67 ◽  
Author(s):  
Iwona Wawer ◽  
Agnieszka Zielińska ◽  
Dorota Maciejewska ◽  
Vera Koleva ◽  
Christina Lozanova
Keyword(s):  
Molecular Structure ◽  
13C Nmr ◽  
Ft Ir ◽  
Semi Empirical

A New Capability for Light Microscopes: Mid infrared Molecular Analysis

2003 ◽  
Vol 11 (4) ◽  
pp. 16-21
Author(s):  
Alan J. Rein
Keyword(s):  
Molecular Analysis ◽  
Infrared Radiation ◽  
Molecular Spectroscopy ◽  
Mid Infrared ◽  
Ft Ir

The merger of molecular spectroscopy with microscopy is certainly not a new concept. Microscope attachments for FT-IR spectrometers have been available for nearly two decades and there are literally thousands of FT-IR spectrometers with these devices currently installed. The vast majority of them are applied to contaminant or forensic oriented problems. As such, the microscopes are often used as sophisticated beam condensers, enabling the spectrometer to focus infrared radiation on the samples that are typically larger than 10 microns.

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