Multi-spectroscopic method study the interaction of anti-inflammatory drug ketoprofen and calf thymus DNA and its analytical application

2011 ◽  
Vol 79 (1) ◽  
pp. 92-96 ◽  
Author(s):  
Hongqin Guo ◽  
Changqun Cai ◽  
Hang Gong ◽  
Xiaoming Chen
Keyword(s):  
Spectroscopic Method ◽  
Analytical Application ◽  
Calf Thymus Dna ◽  
Inflammatory Drug ◽  
Calf Thymus ◽  
Anti Inflammatory

scholarly journals Interaction of indomethacin with calf thymus DNA: a multi-spectroscopic, thermodynamic and molecular modelling approach

MedChemComm ◽  
2017 ◽  
Vol 8 (6) ◽  
pp. 1283-1296 ◽  
Author(s):  
Mohammed Amir Husain ◽  
Hassan Mubarak Ishqi ◽  
Tarique Sarwar ◽  
Sayeed Ur Rehman ◽  
Mohammad Tabish
Keyword(s):  
Acetic Acid ◽  
Acid Derivative ◽  
Molecular Modelling ◽  
Biological Activities ◽  
Calf Thymus Dna ◽  
Calf Thymus ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Modelling Approach

Indomethacin belongs to the acetic acid derivative class of non-steroidal anti-inflammatory drugs with diverse pharmacological and biological activities.

Mixed ligand–silver(i) complexes with anti-inflammatory agents which can bind to lipoxygenase and calf-thymus DNA, modulating their function and inducing apoptosis

Metallomics ◽  
2012 ◽  
Vol 4 (6) ◽  
pp. 545 ◽  
Author(s):  
C. N. Banti ◽  
A. D. Giannoulis ◽  
N. Kourkoumelis ◽  
A. M. Owczarzak ◽  
M. Poyraz ◽  
...  
Keyword(s):  
Mixed Ligand ◽  
Calf Thymus Dna ◽  
Calf Thymus ◽  
Anti Inflammatory

scholarly journals Study on the Interaction of 4'-Hydroxychalcones and their Mannich Derivatives with Calf Thymus DNA by TLC and Spectroscopic Methods, a DNA Cleavage Study

2020 ◽  
Vol 14 (1) ◽  
pp. 122-131
Author(s):  
Zsuzsanna Rozmer ◽  
Aline Bernardes ◽  
Caridad N. Pérez ◽  
Pál Perjési
Keyword(s):  
Cell Lines ◽  
Dna Cleavage ◽  
Binding Constant ◽  
Spectroscopic Method ◽  
Spectrophotometric Titration ◽  
Mannich Bases ◽  
Calf Thymus Dna ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Calf Thymus

Background: Phenolic Mannich bases derived from hydroxychalcones show remarkable cytotoxic potencies towards cancer cell lines. However, the exact mechanism of action is still partially uncleared. Objective: Interaction of two hydroxychalcones and their Mannich derivatives with calf thymus DNA (ctDNA) has been investigated. Methods: Thin-layer chromatography and UV-Vis spectroscopic method were used for studying the interaction. The binding constant has been determined by UV-Vis spectrophotometric titration. The DNA cleavage activity of the compounds was studied by agarose gel electrophoresis. Results: Interaction of the compounds with ctDNA exhibited relatively high intrinsic binding constant (4-5x104 M-1). The results indicate existence of weak, non-covalent interactions between the investigated derivatives with ctDNA. Some compounds showed a slight DNA cleavage activity with pBR322. Conclusion: The obtained results provide additional knowledge on the previously documented cytotoxicity against tumor cell lines of the hydroxychalcones and their Mannich-derivatives.

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