scholarly journals Characteristic differences of cell sheets composed of mesenchymal stem cells with different tissue origins

2019 ◽  
Vol 11 ◽  
pp. 34-40 ◽  
Author(s):  
Mitsuyoshi Nakao ◽  
Daimu Inanaga ◽  
Kenichi Nagase ◽  
Hideko Kanazawa
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Sheets

scholarly journals The Combined Thermoresponsive Cell-Imprinted Substrate, Induced Differentiation, and "KLC Sheet" Formation

2021 ◽  
Author(s):  
Neda Keyhanvar ◽  
Nosratollah Zarghami ◽  
Alexander Seifalian ◽  
Peyman Keyhanvar ◽  
Rana Sarvari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Sheet ◽  
Pdms Substrate ◽  
Cell Sheets ◽  
Dimethyl Siloxane ◽  
Environment Temperature ◽  
Plastic Surface ◽  
Keratinocyte Cell ◽  
Cytokeratin 14

Purpose: Stem cells can exhibit restorative effects with the commitment to functional cells. Cell-imprinted topographies provide adaptable templates and certain dimensions for the differentiation and bioactivity of stem cells. Cell sheet technology using the thermo-responsive polymers detaches the "cell sheets" easier with less destructive effects on the extracellular matrix (ECM). Here, we aim to dictate keratinocyte-like differentiation of mesenchymal stem cells by using combined cell imprinting and sheet technology. Methods: We developed the poly dimethyl siloxane (PDMS) substrate having keratinocyte cell-imprinted topography grafted with the PNIPAAm polymer. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) were cultured on PDMS substrate for 14 days and keratinocyte-like differentiation monitored via the expression of involucrin, P63, and cytokeratin 14. Results: Data showed the efficiency of the current protocol in the fabrication of PDMS molds. The culture of AT-MSCs induced typical keratinocyte morphology and up-regulated the expression of cytokeratin-14, Involucrin, and P63 compared to AT-MSCs cultured on the plastic surface (p<0.05). Besides, KLC sheets were generated once slight changes occur in the environment temperature. Conclusion: These data showed the hypothesis that keratinocyte cell imprinted substrate can orient AT-MSCs toward KLCs by providing a specific niche and topography.

АДАПТАЦИЯ МЕТОДА ИНТЕРФЕРОМЕТРИИ СЛОЯ БИОМОЛЕКУЛ ДЛЯ КОЛИЧЕСТВЕННОЙ ОЦЕНКИ СОДЕРЖАНИЯ ФАКТОРА РОСТА ЭНДОТЕЛИЯ СОСУДОВ В КОНДИЦИОНИРОВАННОЙ КЛЕТОЧНОЙ СРЕДЕ

2020 ◽  
Vol 65 (6) ◽  
pp. 1099-1106
Author(s):  
М.В. Волкова ◽  
◽  
В.В. Бояринцев ◽  
А.В. Трофименко ◽  
С.А. Бирюков ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
High Sensitivity ◽  
Conditioned Media ◽  
Enzyme Linked Immunosorbent Assay ◽  
Growth Media ◽  
Cell Sheets ◽  
Hypoxic Conditions ◽  
Endothelial Growth Factor

Quantitative analysis of cytokines, chemokines, growth factors and other soluble proteins in different biological liquids is routinely performed in contemporary diagnostics and biomedicine research. However, current methods of analysis are time-consuming and include multiple steps. In this study, we have developed a protocol that describes how bio-layer interferometry can be applied to quantify an analyte in several minutes. Conditioned growth media collected from mouse mesenchymal stem cells grown in normoxia or hypoxic conditions in a monolayer fashion, MSC-derived 3D cell sheets and 3D spheroids were used as a model system in which we determined a concentration of vascular endothelial growth factor (VEGF-A). This technique displayed a high sensitivity (down to 0.1 ng of VEGF-A per mL as a minimum). The measured concentrations of VEGF-A in the conditioned media from mesenchymal stem cells turned out to be similar with values determined by the enzyme-linked immunosorbent assay. Using bio-layer interferometry, it was shown that as compared to mesenchymal stem cells grown in monolayer, spheroids and 3D sheets of mesenchymal stem cells produce significantly more VEGF-A (by 2.5-3.0-fold). Thus, due to the developed protocol it was possible to adapt bio-layer interferometry for rapid quantification of growth factors in conditioned media.

Self-assembled cell sheets composed of mesenchymal stem cells and gelatin nanofibers for the treatment of full-thickness wounds

2020 ◽  
Vol 8 (16) ◽  
pp. 4535-4544
Author(s):  
Oanh-Vu Pham-Nguyen ◽  
Ji Un Shin ◽  
Hyesung Kim ◽  
Hyuk Sang Yoo
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Cell Sheet ◽  
Full Thickness ◽  
Cell Sheets ◽  
Self Assembled

Gelatin-layered PCL nanofibrils for 3D cell sheet formation were composed with adipocyte-derived stem cells for wound healing.

scholarly journals The enhancement of human bone marrow derived mesenchymal stem cells (hBMSCS) for scaffold-free cartilage-like cell-sheets

2014 ◽  
Vol 22 ◽  
pp. S487-S488
Author(s):  
M. Itokazu ◽  
H. Mera ◽  
Y. Sato ◽  
H. Nakamura ◽  
M. Takagi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Sheets

Rapid harvesting of stem cell sheets by thermoresponsive bulk poly(N-isopropylacrylamide) (PNIPAAm) nanotopography

2020 ◽  
Vol 8 (19) ◽  
pp. 5260-5270
Author(s):  
Andrew Choi ◽  
Hyungjun Yoon ◽  
Seon Jin Han ◽  
Ji-Ho Lee ◽  
In Hyeok Rhyou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Sheets ◽  
Marrow Mesenchymal Stem Cells ◽  
Novel Concept

A novel concept of utilizing bulk PNIPAAm substrate nanotopography for rapid harvest of human bone marrow mesenchymal stem cells (hBMSCs) sheet. The created nanotogporaphy significantly accelerated the formation of hBMSC layers and eased the detachment of hBMSC sheets.

Sign in / Sign up

Export Citation Format

Share Document