scholarly journals Comparison of the efficacy of cryopreserved human platelet lysate and refrigerated lyophilized human platelet lysate for wound healing

2019 ◽  
Vol 10 ◽  
pp. 1-9 ◽  
Author(s):  
Sharon Claudia Notodihardjo ◽  
Naoki Morimoto ◽  
Natsuko Kakudo ◽  
Toshihito Mitsui ◽  
Tien Minh Le ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Human Platelet Lysate

The effect of human platelet lysate on corneal nerve regeneration

2019 ◽  
pp. bjophthalmol-2019-314408 ◽  
Author(s):  
Chin-Te Huang ◽  
Hsiao-Sang Chu ◽  
Kuo-Chi Hung ◽  
Lily Wei Chen ◽  
Mei-Yun Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Nerve Regeneration ◽  
Neurotrophic Factors ◽  
Ganglion Cells ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Corneal Nerve

AimThis study aimed to test whether human platelet lysate (HPL) has neurotrophic ability for corneal nerve regeneration.MethodsWe measured the neurotrophic factors in human peripheral serum (HPS) and two commercially available HPLs, UltraGRO and PLTMax. In vitro, we compared the growth rates, neuronal differentiation and immunostaining of neuron markers in mouse neuroblastoma cell line (Neuro-2a) and primary culture of mouse trigeminal ganglion cells that were cultivated in different concentrations of fetal bovine serum, HPS and HPL. In vivo, we created corneal wounds on Sprague Dawley rats with a rotating burr and evaluated the effects of topical HPL on wound healing and corneal nerve regeneration by in vivo confocal microscopy and corneal aesthesiometry.ResultsHPLs had significantly higher concentrations of various neurotrophic factors compared with HPS (p<0.05). In Neuro-2a cells, 3% HPL was better at promoting neuronal growth and differentiation compared with HPS at the same concentration. HPL was also found to have superior neurotrophic effects compared with HPS in primary cultures of mouse trigeminal ganglion cells. In vivo, HPL-treated eyes had better corneal epithelial wound healing rate, nerve regeneration length and corneal touch threshold compared with eyes treated with artificial tears (p<0.05).ConclusionHPL has significantly higher concentrations of neurotrophic factors compared with HPS. It showed not only in vitro but also in vivo corneal neurotrophic abilities. Our results suggest that HPL may have a potential role in the treatment of diseases related to corneal nerve damage or degeneration.

Human Platelet Lysate Sustains the Osteogenic/Adipogenic Differentiation Potential of Adipose-Derived Mesenchymal Stromal Cells and Maintains Their DNA Integrity in vitro

2019 ◽  
Vol 207 (3-4) ◽  
pp. 149-164 ◽  
Author(s):  
Yaroslav D. Shansky ◽  
Natalia S. Sergeeva ◽  
Irina K. Sviridova ◽  
Pavel A. Karalkin ◽  
Valentina A. Kirsanova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Cell Morphology ◽  
Stromal Cells ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Dna Integrity ◽  
Differentiation Potential ◽  
Human Platelet Lysate

Human platelet lysate (HPL) is a promising alternative to fetal calf serum (FCS) for the expansion of adipose tissue mesenchymal stromal cells (AT-MSCs) for translational medicine applications. However, some biological effects of HPL are still to be elucidated. We aimed to compare complex characteristics, such as cell morphology, proliferative activity, differentiation potential, and especially monolayer recovery, DNA integrity, and the gene expression pattern, between AT-MSCs cultured with HPL or FCS. Primary AT-MSC cultures were expanded in medium containing FCS or pooled HPL. Cell growth and proliferation were estimated by cell doubling time and the monolayer formation rate, while migration was assessed by wound-healing assay. The capacity for adipogenic and osteogenic differentiation was evaluated by alkaline phosphatase and Oil Red O staining. DNA integrity was evaluated by comet assay, and analysis of gene expression by real-time PCR. Media supplemented with HPL or FCS provided a similar surface immunophenotype, cell morphology (except some cell dimensions and a bigger colony size in HPL), DNA integrity, and rate of wound healing. Meanwhile, AT-MSC proliferated more intensively in HPL-supplemented media (especially at 5% HPL) and had a reduced doubling population time. AT-MSC in HPL had increased adipogenic potential and similar osteogenic potential in comparison with FCS. Our results indicate the feasibility and evident prospects for the use of pooled HPL as an alternative to FCS and safe non-xenogenic growth supplement for ex vivo expansion of clinical-grade AT-MSCs for regenerative medicine purposes.

scholarly journals Expired Platelet Concentrate as a Source of Human Platelet Lysate for Xenogeneic-Free Culture of Human Dermal Fibroblasts

2021 ◽  
Vol 50 (8) ◽  
pp. 2355-2365
Author(s):  
Muhammad Najib Fathi Bin Hassan ◽  
Zheng Yie Yap ◽  
Yee Loong Tang ◽  
Min Hwei Ng ◽  
Jia Xian Law
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Human Platelet ◽  
Defined Medium ◽  
Pathogen Transmission ◽  
Dermal Fibroblasts ◽  
Platelet Lysate ◽  
Human Dermal Fibroblasts ◽  
Chemically Defined Medium ◽  
Human Platelet Lysate

Dermal fibroblasts have been used clinically to promote wound healing and to reduce wrinkles. Most of the time, fetal bovine serum (FBS) is used for the expansion of fibroblasts. In addition, chemically defined medium can also be used for fibroblast expansion. Nonetheless, both FBS and chemically defined medium are not ideal to culture cells that will be used clinically as FBS has the risk of pathogen transmission and induction of xenogeneic immune response whilst chemically defined medium is extremely expensive. In this study, we examine the potential of using human platelet lysate (hPL) prepared from expired platelet concentrates to culture human dermal fibroblasts. For the experiments, fibroblasts were cultured with 5 and 10% hPL, with 10% FBS as the control group to compare the cell morphology, viability, growth rate, extracellular matrix gene expression and wound healing. Results showed that fibroblasts cultured with hPL were more elongated and smaller in size. The cell viability was higher than 90% for all groups. Expansion with 10% hPL significantly shorten the population doubling time compared to the 5% hPL and 10% FBS groups. However, fibroblasts cultured with hPL have lower expression of type I collagen, type III collagen and fibronection as well as slower wound closure. In summary, hPL has the potential to be used as a serum substitute for FBS to expand fibroblasts as it significantly increases the cell proliferation. However, further studies are required to determine if the changes in the ECM gene expression and migration of the hPL-expanded fibroblasts will affect the efficacy of the cells in promoting in vivo wound healing.

scholarly journals Human platelet lysate delivered via an ocular wound chamber for the treatment of corneal epithelial injuries

2021 ◽  
Vol 206 ◽  
pp. 108493
Author(s):  
Gina L. Griffith ◽  
Andrew W. Holt ◽  
Elof Eriksson ◽  
Anthony J. Johnson ◽  
Jennifer S. McDaniel
Keyword(s):  
Human Platelet ◽  
Platelet Lysate ◽  
Corneal Epithelial ◽  
Human Platelet Lysate

scholarly journals Human platelet lysate improves human cord blood derived ECFC survival and vasculogenesis in three dimensional (3D) collagen matrices

2015 ◽  
Vol 101 ◽  
pp. 72-81 ◽  
Author(s):  
Hyojin Kim ◽  
Nutan Prasain ◽  
Sasidhar Vemula ◽  
Michael J. Ferkowicz ◽  
Momoko Yoshimoto ◽  
...  
Keyword(s):  
Cord Blood ◽  
Human Platelet ◽  
Three Dimensional ◽  
Platelet Lysate ◽  
Human Cord Blood ◽  
Collagen Matrices ◽  
Human Platelet Lysate ◽  
3D Collagen
Sign in / Sign up

Export Citation Format

Share Document