scholarly journals A biological study establishing the endotoxin limit for osteoblast and adipocyte differentiation of human mesenchymal stem cells

2018 ◽  
Vol 8 ◽  
pp. 46-57 ◽  
Author(s):  
Yusuke Nomura ◽  
Chie Fukui ◽  
Yuki Morishita ◽  
Yuji Haishima
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipocyte Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Biological Study

scholarly journals The Dietary Antioxidant Piceatannol Inhibits Adipogenesis of Human Adipose Mesenchymal Stem Cells and Limits Glucose Transport and Lipogenic Activities in Adipocytes

2018 ◽  
Vol 19 (7) ◽  
pp. 2081 ◽  
Author(s):  
Christian Carpéné ◽  
Héctor Pejenaute ◽  
Raquel del Moral ◽  
Nathalie Boulet ◽  
Elizabeth Hijona ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Phenolic Compounds ◽  
Glucose Transport ◽  
Adipocyte Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Herbal Remedies ◽  
Metabolic Complications ◽  
Obesity And Diabetes

Phenolic compounds are among the most investigated herbal remedies, as is especially the case for resveratrol. Many reports have shown its anti-aging properties and the ability to reduce obesity and diabetes induced by high-fat diet in mice. However, such beneficial effects hardly translate from animal models to humans. The scientific community has therefore tested whether other plant phenolic compounds may surpass the effects of resveratrol. In this regard, it has been reported that piceatannol reproduces in rodents the anti-obesity actions of its parent polyphenol. However, the capacity of piceatannol to inhibit adipocyte differentiation in humans has not been characterized so far. Here, we investigated whether piceatannol was antiadipogenic and antilipogenic in human preadipocytes. Human mesenchymal stem cells (hMSC), isolated from adipose tissues of lean and obese individuals, were differentiated into mature adipocytes with or without piceatannol, and their functions were explored. Fifty μM of piceatannol deeply limited synthesis/accumulation of lipids in both murine and hMSC-derived adipocytes. Interestingly, this phenomenon occurred irrespective of being added at the earlier or later stages of adipocyte differentiation. Moreover, piceatannol lowered glucose transport into adipocytes and decreased the expression of key elements of the lipogenic pathway (PPARγ, FAS, and GLUT4). Thus, the confirmation of the antiadipogenic properties of piceatanol in vitro warrants the realization of clinical studies for the application of this compound in the treatment of the metabolic complications associated with obesity.

scholarly journals ATF4 licenses C/EBPβ activity in human mesenchymal stem cells primed for adipogenesis

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Daniel M Cohen ◽  
Kyoung-Jae Won ◽  
Nha Nguyen ◽  
Mitchell A Lazar ◽  
Christopher S Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Transcription Factor ◽  
Dna Sequence ◽  
Cell Shape ◽  
Chemical Cues ◽  
Adipocyte Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Seeding Density ◽  
Transcriptional Program

A well-established cascade of transcription factor (TF) activity orchestrates adipogenesis in response to chemical cues, yet how cell-intrinsic determinants of differentiation such as cell shape and/or seeding density inform this transcriptional program remain enigmatic. Here, we uncover a novel mechanism licensing transcription in human mesenchymal stem cells (hMSCs) adipogenically primed by confluence. Prior to adipogenesis, confluency promotes heterodimer recruitment of the bZip TFs C/EBPβ and ATF4 to a non-canonical C/EBP DNA sequence. ATF4 depletion decreases both cell-density-dependent transcription and adipocyte differentiation. Global profiling in hMSCs and a novel cell-free assay reveals that ATF4 requires C/EBPβ for genomic binding at a motif distinct from that bound by the C/EBPβ homodimer. Our observations demonstrate that C/EBPβ bridges the transcriptional programs in naïve, confluent cells and early differentiating pre-adipocytes. Moreover, they suggest that homo- and heterodimer formation poise C/EBPβ to execute diverse and stage-specific transcriptional programs by exploiting an expanded motif repertoire.

scholarly journals Activation of Protein Kinase A and Exchange Protein Directly Activated by cAMP Promotes Adipocyte Differentiation of Human Mesenchymal Stem Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e34114 ◽  
Author(s):  
Bingbing Jia ◽  
Lise Madsen ◽  
Rasmus Koefoed Petersen ◽  
Nathalie Techer ◽  
Reidun Kopperud ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Adipocyte Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Kinase A ◽  
Exchange Protein

scholarly journals Adipocyte differentiation induced using nonspecific siRNA controls in cultured human mesenchymal stem cells

RNA ◽  
2007 ◽  
Vol 13 (8) ◽  
pp. 1179-1183 ◽  
Author(s):  
Y. Xu ◽  
S.-H. Mirmalek-Sani ◽  
F. Lin ◽  
J. Zhang ◽  
R. O.C. Oreffo
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipocyte Differentiation ◽  
Human Mesenchymal Stem Cells
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