scholarly journals Dynamic cultivation with radial flow bioreactor enhances proliferation or differentiation of rat bone marrow cells by fibroblast growth factor or osteogenic differentiation factor

2016 ◽  
Vol 5 ◽  
pp. 17-24 ◽  
Author(s):  
Yuuhei Kanda ◽  
Itsurou Nishimura ◽  
Toru Sato ◽  
Aiko Katayama ◽  
Taichi Arano ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Osteogenic Differentiation ◽  
Fibroblast Growth Factor ◽  
Radial Flow ◽  
Bone Marrow Cells ◽  
Fibroblast Growth ◽  
Dynamic Cultivation ◽  
Radial Flow Bioreactor ◽  
Rat Bone

scholarly journals Effects of different 1-34 parathyroid hormone dosages on fibroblast growth factor-23 secretion in human bone marrow cells following osteogenic differentiation

2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Frank-Peter Tillmann ◽  
Daniela Hofen ◽  
Monika Herten ◽  
Rüdiger Krauspe ◽  
Marcus Jäger
Keyword(s):  
Bone Marrow ◽  
Vitamin D ◽  
Parathyroid Hormone ◽  
Growth Factor ◽  
Osteogenic Differentiation ◽  
Fibroblast Growth Factor ◽  
Bone Marrow Cells ◽  
Fibroblast Growth ◽  
Trap 5B ◽  
Fgf 23

The importance of fibroblast growth factor (FGF)-23 as part of a hormonal bone-kidney-axis has been well established. Lately, FGF-23 has been suggested as an independent risk factor of death in patients on chronic hemodialysis. Hyperparathyroidism is a common feature of advanced kidney failure or end-stage renal disease. The independent effect of elevated parathyroid hormone (PTH) levels on FGF-23 secretion is still a matter of debate and has not yet been studied in an <em>in</em> <em>vitro</em> model of human bone marrow cells (BMC) during osteogenic differentiation. BMC from three different donors were cultivated for 4 weeks in cell cultures devoid of vitamin D either without 1-34 PTH or with PTH concentrations of 10 or 100 pmol/L, respectively. After 28 days, protein expression of the cells was determined by immunocytochemical staining, whereas real time-polymerase chain reaction served to analyze gene expression of several osteoblastic (osteocalcin, RANKL, Runx-2 and ostase) and osteoclastic markers (RANK, TRAP-5b). The concentrations of FGF-23, ostase and TRAP-5b were determined by ELISA at weeks 2, 3 and 4. We found a basal expression of FGF-23 with no increase in FGF-23 secretion after stimulation with 10 pmol/L 1-34 PTH. Stimulation with 100 pmol/L PTH resulted in an increase in FGF-23 expression (14.1±3.6 pg/mL with no PTH, 13.7±4.0 pg/mL with 10 pmol/L, P=0.84 and 17.6±3.4 pg/mL with 100 pmol/L, P=0.047). These results suggest a vitamin D and PTH-independent FGF-23 expression in human BMC after osteogenic stimulation. As only higher PTH levels stimulated FGF-23 expression, a threshold level might be hypothesized.

scholarly journals Human and Rat Bone Marrow-Derived Mesenchymal Stem Cells Differ in Their Response to Fibroblast Growth Factor and Platelet-Derived Growth Factor

2018 ◽  
Vol 24 (23-24) ◽  
pp. 1831-1843
Author(s):  
Donald Lennon ◽  
Luis A. Solchaga ◽  
Rodrigo A. Somoza ◽  
Mark D. Schluchter ◽  
Seunghee Margevicius ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Platelet Derived Growth Factor ◽  
Fibroblast Growth ◽  
Rat Bone

Fibroblast growth factor 2 facilitates the differentiation of transplanted bone marrow cells into hepatocytes

2005 ◽  
Vol 323 (2) ◽  
pp. 221-231 ◽  
Author(s):  
Tsuyoshi Ishikawa ◽  
Shuji Terai ◽  
Yohei Urata ◽  
Yoshio Marumoto ◽  
Koji Aoyama ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Bone Marrow Cells ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Marrow Cells
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