scholarly journals Studying respiratory rhythm generation in a developing bird: Hatching a new experimental model using the classic in vitro brainstem-spinal cord preparation

2016 ◽  
Vol 224 ◽  
pp. 62-70 ◽  
Author(s):  
Michael A. Vincen-Brown ◽  
Kaitlyn C. Whitesitt ◽  
Forrest G. Quick ◽  
Jason Q. Pilarski
Keyword(s):  
Spinal Cord ◽  
Experimental Model ◽  
Respiratory Rhythm ◽  
Rhythm Generation ◽  
Respiratory Rhythm Generation

Are pacemaker properties required for respiratory rhythm generation in adult turtle brain stems in vitro?

2007 ◽  
Vol 293 (2) ◽  
pp. R901-R910 ◽  
Author(s):  
Stephen M. Johnson ◽  
Liana M. Wiegel ◽  
David J. Majewski
Keyword(s):  
Channel Blocker ◽  
Respiratory Rhythm ◽  
Cation Channels ◽  
Neuron Activity ◽  
Synaptic Inhibition ◽  
Dependent Manner ◽  
Rhythm Generation ◽  
Burst Frequency ◽  
Respiratory Rhythm Generation

The role of pacemaker properties in vertebrate respiratory rhythm generation is not well understood. To address this question from a comparative perspective, brain stems from adult turtles were isolated in vitro, and respiratory motor bursts were recorded on hypoglossal (XII) nerve rootlets. The goal was to test whether burst frequency could be altered by conditions known to alter respiratory pacemaker neuron activity in mammals (e.g., increased bath KCl or blockade of specific inward currents). While bathed in artificial cerebrospinal fluid (aCSF), respiratory burst frequency was not correlated with changes in bath KCl (0.5–10.0 mM). Riluzole (50 μM; persistent Na+ channel blocker) increased burst frequency by 31 ± 5% ( P < 0.05) and decreased burst amplitude by 42 ± 4% ( P < 0.05). In contrast, flufenamic acid (FFA, 20–500 μM; Ca2+-activated cation channel blocker) reduced and abolished burst frequency in a dose- and time-dependent manner ( P < 0.05). During synaptic inhibition blockade with bicuculline (50 μM; GABAA channel blocker) and strychnine (50 μM; glycine receptor blocker), rhythmic motor activity persisted, and burst frequency was directly correlated with extracellular KCl (0.5–10.0 mM; P = 0.005). During synaptic inhibition blockade, riluzole (50 μM) did not alter burst frequency, whereas FFA (100 μM) abolished burst frequency ( P < 0.05). These data are most consistent with the hypothesis that turtle respiratory rhythm generation requires Ca2+-activated cation channels but not pacemaker neurons, which thereby favors the group-pacemaker model. During synaptic inhibition blockade, however, the rhythm generator appears to be transformed into a pacemaker-driven network that requires Ca2+-activated cation channels.

Effects of a TRPV1 agonist capsaicin on respiratory rhythm generation in brainstem-spinal cord preparation from newborn rats

2016 ◽  
Vol 469 (2) ◽  
pp. 327-338 ◽  
Author(s):  
Mariho Tani ◽  
Sayumi Kotani ◽  
Chikara Hayakawa ◽  
Shih-Tien Lin ◽  
Saki Irie ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Respiratory Rhythm ◽  
Rhythm Generation ◽  
Newborn Rats ◽  
Respiratory Rhythm Generation

scholarly journals TASK Channels Contribute to the K+-Dominated Leak Current Regulating Respiratory Rhythm Generation In Vitro

2010 ◽  
Vol 30 (12) ◽  
pp. 4273-4284 ◽  
Author(s):  
H. Koizumi ◽  
S. E. Smerin ◽  
T. Yamanishi ◽  
B. R. Moorjani ◽  
R. Zhang ◽  
...  
Keyword(s):  
Respiratory Rhythm ◽  
Rhythm Generation ◽  
Leak Current ◽  
Task Channels ◽  
Respiratory Rhythm Generation
Sign in / Sign up

Export Citation Format

Share Document