New immobilization method of anti-PepD monoclonal antibodies for the detection of Listeria monocytogenes p60 protein – Part A: Optimization of a crosslinked film support based on chitosan and cellulose nanocrystals (CNC)

2020 ◽  
Vol 146 ◽  
pp. 104313 ◽  
Author(s):  
Marie-Christine Etty ◽  
Sabato D'Auria ◽  
Shiv Shankar ◽  
Stephane Salmieri ◽  
Julie Coutu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Listeria Monocytogenes ◽  
Cellulose Nanocrystals ◽  
Protein Part ◽  
Immobilization Method

scholarly journals Prediction of B-Cell Epitopes in Listeriolysin O, a Cholesterol Dependent Cytolysin Secreted by Listeria monocytogenes

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Morris S. Jones ◽  
J. Mark Carter
Keyword(s):  
Monoclonal Antibodies ◽  
Listeria Monocytogenes ◽  
B Cell ◽  
Three Dimensional ◽  
Listeriolysin O ◽  
Molecular Models ◽  
B Cell Epitopes ◽  
Discontinuous Epitopes ◽  
Pathogenic Effects ◽  
Domain 4

Listeria monocytogenes is a gram-positive, foodborne bacterium responsible for disease in humans and animals. Listeriolysin O (LLO) is a required virulence factor for the pathogenic effects of L. monocytogenes. Bioinformatics revealed conserved putative epitopes of LLO that could be used to develop monoclonal antibodies against LLO. Continuous and discontinuous epitopes were located by using four different B-cell prediction algorithms. Three-dimensional molecular models were generated to more precisely characterize the predicted antigenicity of LLO. Domain 4 was predicted to contain five of eleven continuous epitopes. A large portion of domain 4 was also predicted to comprise discontinuous immunogenic epitopes. Domain 4 of LLO may serve as an immunogen for eliciting monoclonal antibodies that can be used to study the pathogenesis of L. monocytogenes as well as develop an inexpensive assay.

scholarly journals Identification of Surface Protein Biomarkers of Listeria monocytogenes via Bioinformatics and Antibody-Based Protein Detection Tools

2016 ◽  
Vol 82 (17) ◽  
pp. 5465-5476 ◽  
Author(s):  
Cathy X. Y. Zhang ◽  
Brian W. Brooks ◽  
Hongsheng Huang ◽  
Franco Pagotto ◽  
Min Lin
Keyword(s):  
Monoclonal Antibodies ◽  
Listeria Monocytogenes ◽  
Surface Protein ◽  
Protein Detection ◽  
Food Samples ◽  
Surface Proteins ◽  
Content Type ◽  
Selective Enrichment ◽  
Wide Range ◽  
Serotype 4B

ABSTRACTThe Gram-positive bacteriumListeria monocytogenescauses a significant percentage of the fatalities among foodborne illnesses in humans. Surface proteins specifically expressed in a wide range ofL. monocytogenesserotypes under selective enrichment culture conditions could serve as potential biomarkers for detection and isolation of this pathogen via antibody-based methods. Our study aimed to identify such biomarkers. Interrogation of theL. monocytogenesserotype 4b strain F2365 genome identified 130 putative or known surface proteins. The homologues of four surface proteins, LMOf2365_0578, LMOf2365_0581, LMOf2365_0639, and LMOf2365_2117, were assessed as biomarkers due to the presence of conserved regions among strains ofL. monocytogeneswhich are variable among otherListeriaspecies. Rabbit polyclonal antibodies against the four recombinant proteins revealed the expression of only LMOf2365_0639 on the surface of serotype 4b strain LI0521 cells despite PCR detection of mRNA transcripts for all four proteins in the organism. Three of 35 monoclonal antibodies (MAbs) to LMOf2365_0639, MAbs M3643, M3644, and M3651, specifically recognized 42 (91.3%) of 46L. monocytogeneslineage I and II isolates grown in nonselective brain heart infusion medium. While M3644 and M3651 reacted with 14 to 15 (82.4 to 88.2%) of 17L. monocytogeneslineage I and II isolates, M3643 reacted with 22 (91.7%) of 24 lineage I, II, and III isolates grown in selective enrichment media (UVM1, modified Fraser, Palcam, and UVM2 media). The three MAbs exhibited only weak reactivities (the optical densities at 414 nm were close to the cutoff value) to some otherListeriaspecies grown in selective enrichment media. Collectively, the data indicate the potential of LMOf2365_0639 as a surface biomarker ofL. monocytogenes, with the aid of specific MAbs, for pathogen detection, identification, and isolation in clinical, environmental, and food samples.IMPORTANCEL. monocytogenesis traditionally divided into at least 12 serotypes. Currently, there are no monoclonal antibodies (MAbs) available that are capable of binding to the surface ofL. monocytogenesstrains representing all 12 serotypes. Such antibodies would be useful and are needed for the development of methods to detect and isolateL. monocytogenesfrom food samples. In our study, we aimed to identify surface proteins that possess regions of well-conserved amino acid sequences among various serotypes and then to employ them as antigen targets (biomarkers) for the development of MAbs. Through bioinformatics and protein expression analysis, we identified one of the four putative surface protein candidates, LMOf2365_0639, encoded by the genome of theL. monocytogenesserotype 4b strain F2365, as a useful surface biomarker. Extensive assessment of 35 MAbs raised against LMOf2365_0639 in our study revealed three MAbs (M3643, M3644, and M3651) that recognized a wide range ofL. monocytogenesisolates.

scholarly journals Monoclonal antibodies with a high degree of specificity for Listeria monocytogenes serotype 4b.

1994 ◽  
Vol 60 (10) ◽  
pp. 3548-3552 ◽  
Author(s):  
S Kathariou ◽  
C Mizumoto ◽  
R D Allen ◽  
A K Fok ◽  
A A Benedict
Keyword(s):  
Monoclonal Antibodies ◽  
Listeria Monocytogenes ◽  
Serotype 4B ◽  
High Degree

Monoclonal Antibodies Directed Against the Flagellar Antigens of Listeria Species and Their Potential in EIA-Based Methods

1987 ◽  
Vol 50 (6) ◽  
pp. 479-484 ◽  
Author(s):  
JEFFREY M. FARBER ◽  
JOAN I. SPEIRS
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibodies ◽  
Listeria Monocytogenes ◽  
Enzyme Immunoassay ◽  
Listeria Innocua ◽  
Milk Samples ◽  
Listeria Ivanovii ◽  
Flagellar Antigen ◽  
Listeria Seeligeri ◽  
Listeria Welshimeri

Monoclonal antibodies directed against antigens of Listeria spp. were produced. Three main classes of immunoglobulins were found that reacted with Listeria strains containing either the A, B, or C flagellar antigen. These antibodies reacted with Listeria monocytogenes, Listeria welshimeri, Listeria seeligeri, Listeria ivanovii and Listeria innocua, but not Listeria grayi, Listeria murrayi or Listeria denitrificans. The monoclones tested did not cross-react with any of the 30 non-Listeria cultures examined, including Staphylococcus aureus and Streptococcus faecalis. Cheese and milk samples naturally-contaminated with L. monocytogenes were found to be positive for Listeria within two working days after initiation by using the monoclonal antibodies in an enzyme immunoassay.

Characterization of New Hybridoma Clones Producing Monoclonal Antibodies Reactive Against Both Live and Heat-Killed Listeria monocytogenes

2007 ◽  
Vol 72 (1) ◽  
pp. M008-M015 ◽  
Author(s):  
Seok A. Heo ◽  
Ramakrishna Nannapaneni ◽  
Robert P. Story ◽  
Michael G. Johnson
Keyword(s):  
Monoclonal Antibodies ◽  
Listeria Monocytogenes

Bead array for Listeria monocytogenes detection using specific monoclonal antibodies

Food Control ◽  
2015 ◽  
Vol 47 ◽  
pp. 462-471 ◽  
Author(s):  
Nitsara Karoonuthaisiri ◽  
Ratthaphol Charlermroj ◽  
Jarinthorn Teerapornpuntakit ◽  
Mallika Kumpoosiri ◽  
Orawan Himananto ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Listeria Monocytogenes
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