Immunoglobulin G recognition with Fab fragments imprinted monolithic cryogels: Evaluation of the effects of metal-ion assisted-coordination of template molecule

2013 ◽  
Vol 73 (6) ◽  
pp. 813-820 ◽  
Author(s):  
Sevgi Asliyuce ◽  
Lokman Uzun ◽  
Ridvan Say ◽  
Adil Denizli
Keyword(s):  
Immunoglobulin G ◽  
Metal Ion ◽  
Template Molecule ◽  
Fab Fragments

The potency of immunoglobulin G fragments for inhibition of interference caused by anti-immunoglobulin antibodies in a monoclonal immunoradiometric assay for thyrotropin.

1988 ◽  
Vol 34 (7) ◽  
pp. 1481-1483 ◽  
Author(s):  
G Csako ◽  
B D Weintraub ◽  
M H Zweig
Keyword(s):  
Immunoglobulin G ◽  
Immunoradiometric Assay ◽  
Igg Antibodies ◽  
Fc Fragment ◽  
Fab Fragments ◽  
Sandwich Type ◽  
Sandwich Assays ◽  
Positive Interference

Abstract Heterophile antibodies in patients' serum may produce false increases in apparent analyte concentrations in "sandwich"-type immunoassays. Using three patients with endogenous anti-mouse IgG antibodies and a two-site mouse monoclonal assay for thyrotropin, we studied the ability of IgG fragments to block this positive interference. Mouse whole IgG and IgG Fc fragment blocked the interference virtually completely; IgG F(ab')2 and Fab fragments did not. Rat and horse immunoglobulin fragments gave variable results. We suggest that sandwich assays formulated with IgG Fab or F(ab')2 fragments may be less susceptible to positive interference by heterophile antibodies. Unlike sera from the three patients, simulated human specimens containing heterologous anti-IgG antibodies showed little or no selectivity in inhibition by IgG fragments, and therefore are not useful to study this phenomenon.

Fab fragments imprinted SPR biosensor for real-time human immunoglobulin G detection

2011 ◽  
Vol 28 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Gizem Ertürk ◽  
Lokman Uzun ◽  
M. Aşkın Tümer ◽  
Rıdvan Say ◽  
Adil Denizli
Keyword(s):  
Real Time ◽  
Immunoglobulin G ◽  
Human Immunoglobulin ◽  
Human Immunoglobulin G ◽  
Fab Fragments ◽  
Spr Biosensor

scholarly journals Detection of Ureaplasma urealyticum by PCR and Biovar Determination by Liquid Hybridization

1998 ◽  
Vol 36 (11) ◽  
pp. 3211-3216 ◽  
Author(s):  
K. Povlsen ◽  
J. S. Jensen ◽  
I. Lind
Keyword(s):  
Immunoglobulin G ◽  
Ureaplasma Urealyticum ◽  
Direct Detection ◽  
Reference Method ◽  
Fab Fragments ◽  
Urease Gene ◽  
Culture Positive

An assay which combines the direct detection of Ureaplasma urealyticum with biovar determination was developed and applied to 618 urogenital specimens. U. urealyticum was detected by inhibitor-controlled PCR. A 429-bp fragment of the urease gene was amplified. The amplicons were labelled with digoxigenin during PCR. Biovar determination was performed by liquid hybridization with biotin-labelled biovar-specific probes, and the hybrids were detected with peroxidase-conjugated sheep anti-digoxigenin immunoglobulin G Fab fragments. Results of PCR and culture for 453 urogenital specimens from women and 105 urethral specimens from men could be compared. Among the specimens from women, 63% were PCR positive as well as culture positive, 0.9% were positive only by PCR, and 4% were positive only by culture. Among the specimens from men, 15% were PCR positive as well as culture positive, 1% were positive only by PCR, and 9% were positive only by culture. By using culture as the reference method, the PCR had a sensitivity of 94% and a specificity of 98% when applied to specimens from women and a sensitivity of 64% and a specificity of 99% when applied to specimens from men. Overall, 80% of the PCR-positive specimens contained biovar 1,13.5% contained biovar 2, and 6.5% contained both biovars.

Sign in / Sign up

Export Citation Format

Share Document