Impact of maternal age on mosaicism rate in preimplantation embryos. A retrospective study

2019 ◽  
Vol 38 ◽  
pp. e62-e63
Author(s):  
C.W. Chan ◽  
C.S.S. Lee ◽  
W.Y. Yap ◽  
Y.X. Lim
Keyword(s):  
Retrospective Study ◽  
Maternal Age ◽  
Preimplantation Embryos

scholarly journals Advanced maternal age perturbs mouse embryo development and alters the phenotype of derived embryonic stem cells

2021 ◽  
pp. 1-11
Author(s):  
Pooja Khurana ◽  
Neil R. Smyth ◽  
Bhavwanti Sheth ◽  
Miguel A. Velazquez ◽  
Judith J. Eckert ◽  
...  
Keyword(s):  
Maternal Age ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Advanced Maternal Age ◽  
Preimplantation Embryos ◽  
Dependent Manner ◽  
Ki 67 ◽  
Altered Expression ◽  
Developmental Potential ◽  
Developmental Mechanisms

Abstract Advanced maternal age (AMA) is known to reduce fertility, increases aneuploidy in oocytes and early embryos and leads to adverse developmental consequences which may associate with offspring lifetime health risks. However, investigating underlying effects of AMA on embryo developmental potential is confounded by the inherent senescence present in maternal body systems further affecting reproductive success. Here, we describe a new model for the analysis of early developmental mechanisms underlying AMA by the derivation and characterisation of mouse embryonic stem cell (mESC-like) lines from naturally conceived embryos. Young (7–8 weeks) and Old (7–8 months) C57BL/6 female mice were mated with young males. Preimplantation embryos from Old dams displayed developmental retardation in blastocyst morphogenesis. mESC lines established from these blastocysts using conventional techniques revealed differences in genetic, cellular and molecular criteria conserved over several passages in the standardised medium. mESCs from embryos from AMA dams displayed increased incidence of aneuploidy following Giemsa karyotyping compared with those from Young dams. Moreover, AMA caused an altered pattern of expression of pluripotency markers (Sox2, OCT4) in mESCs. AMA further diminished mESC survival and proliferation and reduced the expression of cell proliferation marker, Ki-67. These changes coincided with altered expression of the epigenetic marker, Dnmt3a and other developmental regulators in a sex-dependent manner. Collectively, our data demonstrate the feasibility to utilise mESCs to reveal developmental mechanisms underlying AMA in the absence of maternal senescence and with reduced animal use.

Dizygotic to monozygotic twinning ratio at The Royal Women's Hospital, Melbourn 1947–1997, compared with Australian national twinning incidence

Twin Research ◽  
2000 ◽  
Vol 3 (1) ◽  
pp. 12-16
Author(s):  
Stephen Tong
Keyword(s):  
Retrospective Study ◽  
Prospective Studies ◽  
Maternal Age ◽  
Close Agreement ◽  
Significant Decline ◽  
Asian Countries ◽  
Twin Research ◽  
National Statistics ◽  
Monozygotic Twinning ◽  
Human Fertility

AbstractThe incidence of dizygotic (Dz) twinning can be used as an index of natural human fertility. A retrospective study was done at The Royal Women's Hospital, Australia, to see whether the dizygotic to monozygotic (Mz) twinning ratio from one hospital can accurately reflect the national incidence of Dz twinning. The yearly twinning incidence from 1947–1997 was expressed as a Dz:Mz ratio, standardised for maternal age and plotted against previously published national statistics. The proportion of mothers born in Asia (of both singleton and multiples) between 1983–1997 was analysed to see whether different racial mixes might influence twinning trends. There were 5275 twins born of known sex and maternal age between 1947–1997. The agestandardised Dz:Mz ratio increased non-significantly from 1.39 in 1947 to 2.29 in 1953 (P = 0.08), underwent a significant decline to 0.73 in 1977, then remained stable until 1997 (P > 0.05). The same trends were also apparent when the data was pooled into 2-year groups with the increase from 1947/48–1953/54 becoming highly significant (P < 0.009). These trends observed in the hospital-based data were in close agreement with those found in the national statistics, with the exception of a rise in 1977–1982 only reflected in the Australia-wide data. In 1993, 2.6% of mothers were born in Asian countries; by 1997, this had risen to 10.6%. We found that the Dz:Mz ratio from one hospital closely reflects national twinning trends. Prospective studies must account for race, and would need around 200–300 twin pairs per year to minimise fluctuations of the ratio. Twin Research (2000) 3, 12–16.

scholarly journals Factors affecting clinical manifestation of chromosomal imbalance in carriers of segmental autosomal mosaicism: differential impact of gender

2022 ◽  
Author(s):  
Natalia V. Kovaleva ◽  
Philip D. Cotter
Keyword(s):  
Pregnancy Outcomes ◽  
Maternal Age ◽  
Chromosomal Rearrangements ◽  
Clinical Manifestations ◽  
Female Gender ◽  
Normal Pregnancy ◽  
Paternal Age ◽  
Preimplantation Embryos ◽  
Chromosomal Imbalance ◽  
Male Predominance

Abstract Mosaicism for unbalanced chromosomal rearrangements segmental mosaicism (SM) is rare, both in patients referred for cytogenetic testing and in prenatal diagnoses. In contrast, in preimplantation embryos SM is a frequent finding and, therefore, is even more challenging. However, there is no consistency among results of published studies on the clinical outcomes of embryos with SM, primarily due to the small number of reported cases. Moreover, there is the problem of predicting the potential for the optimal development of a mosaic embryo to a healthy individual. Therefore, we suggested comparing factors predisposing to favorable and poor prognoses, identified in postnatal and prenatal cohorts of SM carriers, with those obtained from studies on preimplantation embryos. We analyzed 580 published cases of SM including (i) postnatally diagnosed affected carriers, (ii) clinically asymptomatic carriers, (iii) prenatally diagnosed carriers, and (iv) miscarriages. We observed a concordance with preimplantation diagnoses regarding the clinical significance of the extent of mosaicism as well as a predominance of deletions over other types of rearrangements. However, there is no concordance regarding excessive involvement of chromosomes 1, 5, and 9 in unbalanced rearrangements and a preferential involvement of larger chromosomes compared to short ones. Paternal age was not found to be associated with SM in postnatally disease-defined individuals. We have identified maternal age and preferential involvement of chromosome 18 in rearrangements associated with clinical manifestations. Male predominance was found among normal pregnancy outcomes and among disease-defined carriers of rearrangements resulting in a gain of genomic material. Female predominance was found among abnormal pregnancy outcomes, among disease-defined carriers of loss and gain/loss rearrangements, and among transmitting carriers of gonadal SM, both affected and asymptomatic. According to data obtained from “post-embryo” studies, clinical manifestations of chromosomal imbalance are associated with a high proportion of abnormal cells, female gender, the type of rearrangement and involved chromosome(s), and maternal age. We believe these data are instructive in the challenging medical genetic counseling of parents faced with no option other than transfer of an embryo with segmental mosaicism.

Clinical evaluation of NIPS for women at advanced maternal age: a multicenter retrospective study

2018 ◽  
Vol 32 (24) ◽  
pp. 4080-4085
Author(s):  
Bin Yu ◽  
Hong Li ◽  
Ying-Ping Chen ◽  
Bin Zhang ◽  
Ying Xue ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Clinical Evaluation ◽  
Maternal Age ◽  
Advanced Maternal Age

scholarly journals The Correlation of Maternal Age and the Incidence of Preeclampsia at Aura Syifa Hospital

2021 ◽  
Vol 8 (3) ◽  
pp. 368-372
Author(s):  
Anggita Retno Sari ◽  
Ira Titisari ◽  
Eny Sendra
Keyword(s):  
Retrospective Study ◽  
Medical Records ◽  
Random Sampling ◽  
Maternal Age ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Reproductive Age ◽  
Chi Square ◽  
Age Factor ◽  
Risk Of Preeclampsia

Preeclampsia is a condition when hypertension and proteinuria occurs after 20 weeks of pregnancy. The exact cause of preeclampsia is currently unknown, but many factors influence the occurrence of preeclampsia, especially the age factor. This study was aimed to determine the correlation between maternal age and the incidence of preeclampsia. This study was analytical survey designed with retrospective study method. From Aura Syifa hospital, 142 medical records in of pregnant women in 2017 were taken 105 samples by using simple random sampling technique. The result of this study indicated that delivered mothers who were in reproductive age (20-35 years old), 25.8% of them had preeclampsia and 31,1% had severe preeclampsia. The data analyzed by suing chi-square correlation and the result was ρ (0.00) < α (0.05) which meant that there was a correlation between maternal age and the incidence of preeclampsia. It would be better for couples planning pregnancy in healthy reproductive age for minimize the risk of preeclampsia.

scholarly journals Obstetric outcomes of twin pregnancies at advanced maternal age: A retrospective study

2018 ◽  
Vol 57 (1) ◽  
pp. 64-67 ◽  
Author(s):  
Caixia Zhu ◽  
Malie Wang ◽  
Gang Niu ◽  
Juan Yang ◽  
Zilian Wang
Keyword(s):  
Retrospective Study ◽  
Maternal Age ◽  
Advanced Maternal Age ◽  
Obstetric Outcomes ◽  
Twin Pregnancies

Maternal age triggers the formation of chromosomal losses more than gains and/or segmental aneuploidies in preimplantation embryos

2018 ◽  
Vol 36 ◽  
pp. e19-e20
Author(s):  
Cagri Ogur ◽  
Meral Gultomruk ◽  
Julide Caferler ◽  
Betul Capar ◽  
Necati Findikli ◽  
...  
Keyword(s):  
Maternal Age ◽  
Preimplantation Embryos
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