scholarly journals Residual ethylene glycol and dimethyl sulphoxide concentration in human ovarian tissue during warming/thawing steps following cryopreservation

2017 ◽  
Vol 35 (3) ◽  
pp. 311-313 ◽  
Author(s):  
Yusuke Nakamura ◽  
Ryuichiro Obata ◽  
Noriyuki Okuyama ◽  
Nobuya Aono ◽  
Tomoko Hashimoto ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Ovarian Tissue ◽  
Dimethyl Sulphoxide ◽  
Human Ovarian Tissue

Fertility of mice following receipt of ovaries slow cooled in dimethyl sulphoxide or ethylene glycol is largely independent of cryopreservation equilibration time and temperature

2003 ◽  
Vol 15 (8) ◽  
pp. 407 ◽  
Author(s):  
M. Snow ◽  
S.-L. Cox ◽  
G. Jenkin ◽  
J. Shaw
Keyword(s):  
Ethylene Glycol ◽  
Ovarian Tissue ◽  
Dimethyl Sulphoxide ◽  
Equilibration Time ◽  
Control Groups ◽  
Cryoprotective Agents ◽  
Adult Recipient ◽  
Mouse Pup ◽  
And Control ◽  
Transplantation Procedures

Cryopreservation procedures generally depend on both the cryoprotectant used and the equilibration conditions to which the material is exposed. The aim of the present study was to examine the effect of cryoprotectants (dimethyl sulphoxide (DMSO) and ethylene glycol (EG)) and equilibration conditions (0, 30 or 120 min at 0°C or 120 min at room temperature) on the fertility of mice receiving cryopreserved mouse ovaries. The study compared the fertility of cryopreserved Day 14 mouse pup ovaries following grafting to adult recipient mice for 4 months. There was no effect of the cryoprotectant or equilibration condition used on the interval to the first plugging/mating or on the interval to the birth of the first litter, the size of litters, the number of litters produced or the total number of offspring produced. Despite this, when compared with control females (untreated, sham and fresh transplant) the cryopreservation and transplantation procedures delayed fertility. However, the size of litters was equivalent for all cryopreserved and control groups (P > 0.05). The results show that, for the equilibration conditions examined, DMSO and EG are equally efficient cryoprotective agents for mouse ovarian tissue.

Evaluation of a new freezing protocol containing 20% dimethyl sulphoxide concentration to cryopreserve human ovarian tissue

2018 ◽  
Vol 37 (6) ◽  
pp. 653-665 ◽  
Author(s):  
Miguel Gallardo ◽  
Fernanda Paulini ◽  
Ariadna Corral ◽  
Marcin Balcerzyk ◽  
Carolina M. Lucci ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Dimethyl Sulphoxide ◽  
Human Ovarian Tissue

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model

scholarly journals THE CONVERSION OF TESTOSTERONE-3-C14 TO C14-ESTRADIOL-17β BY HUMAN OVARIAN TISSUE

1956 ◽  
Vol 221 (2) ◽  
pp. 931-941
Author(s):  
Billy Baggett ◽  
Lewis L. Engel ◽  
Kenneth Savard ◽  
Ralph I. Dorfman
Keyword(s):  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Estradiol 17Β

ORIGINAL ARTICLE: Development of Human Graafian Follicles Following Transplantation of Human Ovarian Tissue into NOD/SCID/γcnull Mice

2008 ◽  
Vol 60 (6) ◽  
pp. 534-540 ◽  
Author(s):  
Yukihiro Terada ◽  
Yumi Terunuma-Sato ◽  
Tomoko Kakoi-Yoshimoto ◽  
Hisataka Hasegawa ◽  
Tomohisa Ugajin ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Human Ovarian Tissue

Both host and graft vessels contribute to revascularization of xenografted human ovarian tissue in a murine model

2010 ◽  
Vol 93 (5) ◽  
pp. 1676-1685 ◽  
Author(s):  
Anne-Sophie Van Eyck ◽  
Caroline Bouzin ◽  
Olivier Feron ◽  
Lydia Romeu ◽  
Anne Van Langendonckt ◽  
...  
Keyword(s):  
Murine Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue

Direct metabolism of 7α-3H-dehydroepiandrosteron-35S-sulphate in human ovarian tissue

1967 ◽  
Vol 23 (10) ◽  
pp. 851-852 ◽  
Author(s):  
P. Knapstein ◽  
F. Wendlberger ◽  
G. W. Oertel
Keyword(s):  
Ovarian Tissue ◽  
Human Ovarian Tissue
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