scholarly journals Is nuclear ploidy status associated with mitochondrial DNA (mtDNA) load in human embryos?

2016 ◽  
Vol 33 ◽  
pp. e2
Author(s):  
N. Arrach ◽  
S.H. Wood ◽  
E.S. Sills
Keyword(s):  
Mitochondrial Dna ◽  
Human Embryos ◽  
Nuclear Ploidy ◽  
Ploidy Status

Mitochondrial DNA is significantly increased in aneuploid human embryos

2010 ◽  
Vol 94 (4) ◽  
pp. S88-S89 ◽  
Author(s):  
J. Su ◽  
X. Tao ◽  
G. Baglione ◽  
N.R. Treff ◽  
R.T. Scott
Keyword(s):  
Mitochondrial Dna ◽  
Human Embryos

scholarly journals Mitochondrial DNA Copy Number in Cleavage Stage Human Embryos—Impact on Infertility Outcome

2022 ◽  
Vol 44 (1) ◽  
pp. 273-287
Author(s):  
Amira Podolak ◽  
Joanna Liss ◽  
Jolanta Kiewisz ◽  
Sebastian Pukszta ◽  
Celina Cybulska ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Copy Number ◽  
Human Reproduction ◽  
Preimplantation Embryos ◽  
Human Embryos ◽  
Embryo Morphology ◽  
Cleavage Stage ◽  
Mtdna Copy Number ◽  
Developmental Potential ◽  
Mitochondrial Dna Copy Number

A retrospective case control study was undertaken at the molecular biology department of a private center for reproductive medicine in order to determine whether any correlation exists between mitochondrial DNA (mtDNA) content of cleavage-stage preimplantation embryos and their developmental potential. A total of 69 couples underwent IVF treatment (averaged women age: 36.5, SD 4.9) and produced a total of 314 embryos. A single blastomere was biopsied from each embryo at the cleavage stage (day-3 post-fertilization) subjected to low-pass next generation sequencing (NGS), for the purpose of detecting aneuploidy. For each sample, the number of mtDNA reads obtained after analysis using NGS was divided by the number of reads attributable to the nuclear genome. The mtDNA copy number amount was found to be higher in aneuploid embryos than in those that were euploid (mean mtDNA ratio ± SD: 6.3 ± 7.5 versus 7.1 ± 5.8, p < 0.004; U Mann–Whitney test), whereas no statistically significant differences in mtDNA content were seen in relation to embryo morphology (6.6 ± 4.8 vs. 8.5 ± 13.6, p 0.09), sex (6.6 ± 4.1 vs. 6.2 ± 6.8, p 0.16), maternal age (6.9 ± 7.8 vs. 6.7 ± 4.5, p 0.14) or its ability to implant (7.4 ± 6.6 vs. 5.1 ± 4.6, p 0.18). The mtDNA content cannot serve as a useful biomarker at this point in development. However, further studies investigating both quantitative and qualitative aspects of mtDNA are still required to fully evaluate the relationship between mitochondrial DNA and human reproduction.

scholarly journals DNA methylation is associated with ploidy status and patient age in human embryos

2018 ◽  
Vol 110 (4) ◽  
pp. e80
Author(s):  
X. Tao ◽  
Y. Zhan ◽  
K.L. Scott ◽  
R. Scott ◽  
E. Seli
Keyword(s):  
Dna Methylation ◽  
Human Embryos ◽  
Patient Age ◽  
Patient Âgé ◽  
Ploidy Status

scholarly journals Mitochondrial DNA content is associated with ploidy status, maternal age, and oocyte maturation methods in mouse blastocysts

2017 ◽  
Vol 34 (12) ◽  
pp. 1587-1594 ◽  
Author(s):  
Xin Tao ◽  
Jessica N. Landis ◽  
Rebecca L. Krisher ◽  
Francesca E. Duncan ◽  
Elena Silva ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Oocyte Maturation ◽  
Dna Content ◽  
Maternal Age ◽  
Mitochondrial Dna Content ◽  
Ploidy Status

scholarly journals The Association of Kinetic Variables with Blastocyst Development and Ploidy Status

2021 ◽  
Author(s):  
Francesca Pennetta ◽  
Cristina Lagalla ◽  
Raffaella Sciajno ◽  
Nicoletta Tarozzi ◽  
Marco Nadalini ◽  
...  
Keyword(s):  
Blastocyst Stage ◽  
Human Embryos ◽  
Blastocyst Development ◽  
Developmental Potential ◽  
Logistic Regression Models ◽  
Kolmogorov Smirnov ◽  
Ploidy Changes ◽  
Study Hypothesis ◽  
Ploidy Status ◽  
Smirnov Test

Background: Despite a plethora of studies conducted so far, a debate is still unresolved as to whether TLM can identify predictive kinetic biomarkers or algorithms universally applicable. Therefore, this study aimed to elucidate if there is a relationship between kinetic variables and ploidy status of human embryos or blastocyst developmental potential. Methods: For conducting this retrospective cohort study, the normal distribution of data was verified using Kolmogorov-Smirnov test with the Lilliefors’ amendment and the Shapiro-Wilk test. Kinetic variables were expressed as median and quartiles (Q1, Q2, Q3, Q4). Mann-Whitney U-test was used to compare the median values of parameters. Univariate and multiple logistic regression models were used to assess relationship between blastocyst developmental potential or ploidy status and kinetics. Several confounding factors were also assessed. Results: Blastocyst developmental potential was positively correlated with the t4-t3 interval (s2) (OR=1.417, 95% CI of 1.288-1.560). s2 median value was significantly different between high- and low-quality blastocysts (0.50 and 1.33 hours post-insemination, hpi, respectively; p=0.003). In addition, timing of pronuclear appearance (tPNa) (OR=1.287; 95% CI of 1.131-1.463) had a significant relationship with ploidy changes. The median value of tPNa was statistically different (p=0.03) between euploid and aneuploid blastocysts (Euploid blastocysts=8.9 hpi; aneuploid blastocysts=10.3 hpi).  Conclusion: The present findings are in line with the study hypothesis that kinetic analysis may reveal associations between cleavage patterns and embryo development to the blastocyst stage and ploidy status.

scholarly journals Paternal mitochondrial DNA present in human embryos

2018 ◽  
Vol 110 (4) ◽  
pp. e146-e147
Author(s):  
C. Fischer ◽  
R. Prosser ◽  
R. Lobo ◽  
D. Egli
Keyword(s):  
Mitochondrial Dna ◽  
Human Embryos

scholarly journals No association of mitochondrial DNA levels in trophectodermal cells with the developmental competence of the blastocyst and pregnancy outcomes

2019 ◽  
Author(s):  
G Ritu ◽  
Geetha Veerasigamani ◽  
Mohammed C. Ashraf ◽  
Sankalp Singh ◽  
Saniya Laheri ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Clinical Outcomes ◽  
Live Birth ◽  
Maternal Age ◽  
Small Sample ◽  
Developmental Competence ◽  
Significant Difference ◽  
Blastocyst Quality ◽  
Implantation Rates ◽  
Ploidy Status

AbstractStudy questionCan mitochondrial DNA (mtDNA) levels in trophectodermal cells of the blastocyst predict the blastocyst quality, ploidy status, implantation rate and clinical outcomes?Summary answermtDNA levels in trophectodermal cells of the blastocyst do not associate with the blastocyst quality, ploidy status, implantation potential and clinical outcomes, but can differentiate between aneuploid and euploid blastocysts.What we already knowmtDNA levels in the trophectodermal cells have been suggested to be associated with blastocyst morphology, ploidy and implantation rates, and has been proposed as biomarker to access blastocyst quality and predict clinical outcomes. However, discrepancies exist if mtDNA levels could serve as a marker for the same.Study design and durationRetrospective analysis of mtDNA levels in trophectodermal cells obtained from blastocysts undergoing preimplantation genetic testing for aneuploidy (PGT-A) at Craft Hospital & Research Center, Kerala from January 2016-July 2017.Participants/materials and methodsStudy included data from 287 blastocyst from (61) couples who underwent PGT-A using next generation sequencing (NGS). Levels of mtDNA in trophectodermal cells of the blastocyst were estimated by the NGS. Comparison of mtDNA levels with maternal age, blastocyst morphology, ploidy status, implantation rates, miscarriage rates and live birth rate was done.Main resultsThe levels of mtDNA in the trophectoderm of the blastocyst did not correlate with maternal age. There was no significant difference in the mtDNA levels between grade 1 and grade 2 blastocyst. Euploid blastocyst had significantly lower amounts of mtDNA levels in trophectodermal cells of the blastocyst were compared to aneuploid blastocyst. No significant differences were seen between mtDNA levels and implanting and non-implanting blastocysts or those resulted into miscarriage or live birth.LimitationsThe study is limited by a small sample size and hence type II error cannot be ruled out.Wider ImplicationsThe study does not support the potential use of mtDNA levels in the trophectodermal cells as biomarker for blastocyst quality and predicting clinical outcomes needs.Study funding/competing interest(s)There is no external funding for the study. There is no conflict of interest.

scholarly journals Cleavage stage mitochondrial DNA is correlated with preimplantation human embryo development and ploidy status

2019 ◽  
Vol 36 (9) ◽  
pp. 1847-1854 ◽  
Author(s):  
Aşina Bayram ◽  
Neelke De Munck ◽  
Ibrahim Elkhatib ◽  
Ana Arnanz ◽  
Alberto Liñán ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Embryo Development ◽  
Human Embryo ◽  
Cleavage Stage ◽  
Human Embryo Development ◽  
Ploidy Status
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