scholarly journals In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn

2016 ◽  
Vol 48 (4) ◽  
pp. 267-273 ◽  
Author(s):  
Araceli M. Ramos ◽  
Marcela Gally ◽  
Gala Szapiro ◽  
Tatiana Itzcovich ◽  
Maira Carabajal ◽  
...  
Keyword(s):  
Cell Wall ◽  
Causative Agent ◽  
Enzyme Production ◽  
Macrophomina Phaseolina ◽  
In Vitro Growth ◽  
Charcoal Rot ◽  
Degrading Enzyme ◽  
Cell Wall Degrading Enzyme

Evaluation of plant cell wall degrading enzyme production by Clostridium thermocellum B8 in the presence of raw agricultural wastes

2015 ◽  
Vol 105 ◽  
pp. 97-105 ◽  
Author(s):  
Pedro Ricardo V. Hamann ◽  
Dayane L. Serpa ◽  
Amanda Souza Barreto da Cunha ◽  
Brenda R. de Camargo ◽  
Karen Ofuji Osiro ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Clostridium Thermocellum ◽  
Enzyme Production ◽  
Plant Cell Wall ◽  
Agricultural Wastes ◽  
Degrading Enzyme ◽  
Cell Wall Degrading Enzyme

scholarly journals Pneumolysin Localizes to the Cell Wall of Streptococcus pneumoniae

2009 ◽  
Vol 191 (7) ◽  
pp. 2163-2168 ◽  
Author(s):  
Katherine E. Price ◽  
Andrew Camilli
Keyword(s):  
Cell Wall ◽  
Streptococcus Pneumoniae ◽  
Otitis Media ◽  
Mouse Models ◽  
Western Blotting ◽  
Causative Agent ◽  
Signal Peptide ◽  
Cell Fractionation ◽  
In Vitro Growth

ABSTRACT Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene has been deleted are severely attenuated in mouse models of infection. In contrast to all other members of the cholesterol-dependent cytolysin family, Ply lacks a signal peptide for export. Instead, Ply has been hypothesized to be released upon autolysis or, alternatively, via a nonautolytic mechanism that remains ill defined. We determined by use of cell fractionation and Western blotting that, during in vitro growth, exported Ply is localized primarily to the cell wall compartment in 18 different serotypes in the absence of detectable cell lysis. Hemolytic assays revealed that this cell wall-localized Ply is active. Additionally, cell wall-localized Ply is accessible to extracellular protease and is detergent releasable.

scholarly journals The avian haemophili.

1989 ◽  
Vol 2 (3) ◽  
pp. 270-277 ◽  
Author(s):  
P J Blackall
Keyword(s):  
Causative Agent ◽  
Protective Immunity ◽  
In Vitro Growth ◽  
Phenotypic Properties ◽  
Infectious Coryza ◽  
The Common ◽  
Recent Attention

There are four currently recognized taxa to accommodate the avian haemophili: Haemophilus paragallinarum, Pasteurella avium, Pasteurella volantium, and Pasteurella species A (the last three being formerly united as Haemophilus avium). A range of other taxa has also been recognized, but they have been neither named nor assigned to a genus. All of these various taxa, legitimate and otherwise, have the common characteristic of requiring V factor, but not X factor, for in vitro growth. Several recent studies have established the phenotypic properties that allow the differentiation of the recognized taxa, both named and unnamed. The serological properties of H. paragallinarum, the causative agent of infectious coryza of chickens, has received considerable recent attention. In contrast, many questions on the pathogenicity and virulence mechanisms of H. paragallinarum remain unanswered. Another area requiring further work is the identification of those antigens responsible for inducing protective immunity in vaccinated or naturally infected chickens.

scholarly journals Effect of cell wall degrading enzyme and skin contact time on the brewing characteristics of Cheongsoo grape

2013 ◽  
Vol 20 (6) ◽  
pp. 846-853 ◽  
Author(s):  
Jin-A Jeon ◽  
Seo-Jun Park ◽  
Soo-Hwan Yeo ◽  
Ji-Ho Choi ◽  
Han-Seok Choi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Contact Time ◽  
Degrading Enzyme ◽  
Cell Wall Degrading Enzyme ◽  
Skin Contact

scholarly journals Innovative microscale workflow from fungi cultures to Cell Wall‐Degrading Enzyme screening

2019 ◽  
Vol 12 (6) ◽  
pp. 1286-1292 ◽  
Author(s):  
Roxane Raulo ◽  
Egon Heuson ◽  
Ali Siah ◽  
Vincent Phalip ◽  
Renato Froidevaux
Keyword(s):  
Cell Wall ◽  
Degrading Enzyme ◽  
Cell Wall Degrading Enzyme ◽  
Enzyme Screening

scholarly journals Fungiculture in Termites Is Associated with a Mycolytic Gut Bacterial Community

mSphere ◽  
2019 ◽  
Vol 4 (3) ◽  
Author(s):  
Haofu Hu ◽  
Rafael Rodrigues da Costa ◽  
Bo Pilgaard ◽  
Morten Schiøtt ◽  
Lene Lange ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacterial Community ◽  
Plant Cell ◽  
Functional Annotation ◽  
Plant Cell Wall ◽  
Fungal Cell ◽  
Degrading Enzyme ◽  
Cell Wall Degrading Enzyme ◽  
Degrading Enzymes ◽  
Termite Gut

ABSTRACT Termites forage on a range of substrates, and it has been suggested that diet shapes the composition and function of termite gut bacterial communities. Through comparative analyses of gut metagenomes in nine termite species with distinct diets, we characterize bacterial community compositions and use peptide-based functional annotation method to determine biomass-degrading enzymes and the bacterial taxa that encode them. We find that fungus-growing termite guts have relatively more fungal cell wall-degrading enzyme genes, while wood-feeding termite gut communities have relatively more plant cell wall-degrading enzyme genes. Interestingly, wood-feeding termite gut bacterial genes code for abundant chitinolytic enzymes, suggesting that fungal biomass within the decaying wood likely contributes to gut bacterial or termite host nutrition. Across diets, the dominant biomass-degrading enzymes are predominantly coded for by the most abundant bacterial taxa, suggesting tight links between diet and gut community composition, with the most marked difference being the communities coding for the mycolytic capacity of the fungus-growing termite gut. IMPORTANCE Understanding functional capacities of gut microbiomes is important to improve our understanding of symbiotic associations. Here, we use peptide-based functional annotation to show that the gut microbiomes of fungus-farming termites code for a wealth of enzymes that likely target the fungal diet the termites eat. Comparisons to other termites showed that fungus-growing termite guts have relatively more fungal cell wall-degrading enzyme genes, whereas wood-feeding termite gut communities have relatively more plant cell wall-degrading enzyme genes. Across termites with different diets, the dominant biomass-degrading enzymes are predominantly coded for by the most abundant bacterial taxa, suggesting tight links between diet and gut community compositions.

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