scholarly journals The Role of Chromatin Structure in Gene Regulation of the Human Malaria Parasite

2017 ◽  
Vol 33 (5) ◽  
pp. 364-377 ◽  
Author(s):  
Gayani Batugedara ◽  
Xueqing M. Lu ◽  
Evelien M. Bunnik ◽  
Karine G. Le Roch
Keyword(s):  
Gene Regulation ◽  
Chromatin Structure ◽  
Malaria Parasite ◽  
Human Malaria Parasite ◽  
Human Malaria

scholarly journals Genetic alteration of ferredoxin NADP+-reductase or cysteine desulfurase in piperaquine-resistant Plasmodium berghei restores susceptibility to lumefantrine and abolishes the impact of probenecid, verapamil, and cyproheptadine

2019 ◽  
Author(s):  
Fagdéba David Bara ◽  
Loise Ndung’u ◽  
Noah Machuki Onchieku ◽  
Beatrice Irungu ◽  
Simplice Damintoti Karou ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Berghei ◽  
Malaria Parasite ◽  
Drug Action ◽  
Cysteine Desulfurase ◽  
Human Malaria Parasite ◽  
Human Malaria ◽  
Essential Components ◽  
The Impact

AbstractChemotherapy remains central in the control of malaria; however, resistance has consistently thwarted these efforts. Currently, lumefantrine (LM), and piperaquine (PQ) drugs, are essential components in the mainstay artemisinin-based therapies used for the treatment of malaria globally. Using LM and PQ-resistant Plasmodium berghei, we measured the effect of known chemosensitizers: probenecid, verapamil, or cyproheptadine on the activity of LM or PQ. Using PlasmoGEM vectors, we then evaluated the impact of deleting cysteine desulfurase (SufS) or over-expressing Ferredoxin NADP+ reductase (FNR), genes that mediate drug action. Our data showed that, only cyproheptadine at 5mgkg−1 restored LM activity by above 65% against the LM-resistant parasites (LMR) but failed to reinstate PQ activity against the PQ-resistant parasites (PQR). Whereas the PQR had lost significant susceptibility to LM, the three chemosensitizers; cyproheptadine, probenecid, and verapamil, restored LM potency against the PQR by above 70%, 60%, and 55% respectively. We thus focused on LM resistance in PQR. Deletion of the SufS or overexpression of the FNR genes in the PQR abolished the impact of the chemosensitizers on the LM activity, and restored the susceptibility of the PQR parasites to LM. Taken together, we demonstrate the association between SufS or FNR genes with the action of LM and chemosensitizers in PQR parasites. There is, however, need to interrogate the impact of the chemosensitizers and the role of SufS or FNR genes on LM action in the human malaria parasite, Plasmodium falciparum.

scholarly journals Nascent RNA sequencing reveals mechanisms of gene regulation in the human malaria parasite Plasmodium falciparum

2017 ◽  
Vol 45 (13) ◽  
pp. 7825-7840 ◽  
Author(s):  
Xueqing Maggie Lu ◽  
Gayani Batugedara ◽  
Michael Lee ◽  
Jacques Prudhomme ◽  
Evelien M. Bunnik ◽  
...  
Keyword(s):  
Gene Regulation ◽  
Plasmodium Falciparum ◽  
Rna Sequencing ◽  
Malaria Parasite ◽  
Human Malaria Parasite ◽  
Human Malaria ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum ◽  
Nascent Rna

scholarly journals Plasmodium knowlesi: An Important Yet Overlooked Human Malaria Parasite

2009 ◽  
Vol 84 (7) ◽  
pp. 664
Author(s):  
Balaji Yegneswaran ◽  
David Alcid ◽  
Janani Mohan
Keyword(s):  
Malaria Parasite ◽  
Plasmodium Knowlesi ◽  
Human Malaria Parasite ◽  
Human Malaria
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