scholarly journals Generation and evaluation of a vaccine candidate of attenuated and heat-resistant genotype VIII Newcastle disease virus

2020 ◽  
Vol 99 (7) ◽  
pp. 3437-3444 ◽  
Author(s):  
Baoyang Ruan ◽  
Qian Liu ◽  
Yin Chen ◽  
Xiaosai Niu ◽  
Xuefeng Wang ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Candidate ◽  
Resistant Genotype ◽  
Heat Resistant

scholarly journals Development and pre-clinical evaluation of Newcastle disease virus-vectored SARS-CoV-2 intranasal vaccine candidate

2021 ◽  
Author(s):  
Manolo Fernandez Díaz ◽  
Katherine Calderon ◽  
Aldo Rojas-Neyra ◽  
Vikram N. Vakharia ◽  
Ricardo Choque-Guevara ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Candidate ◽  
Allantoic Fluid ◽  
Cellular Damage ◽  
Hamster Model ◽  
Golden Syrian Hamster ◽  
Oncological Treatment

ABSTRACTThe COVID-19 pandemic has claimed the lives of millions of people worldwide and threatens to become an endemic problem, therefore the need for as many types of vaccines as possible is of high importance.Because of the millions of doses required, it is desirable that vaccines are not only safe and effective, but also easy to administer, store, and inexpensive to produce.Newcastle Disease Virus (NDV) is responsible for a respiratory disease in chickens. It has no pathogenic homologue in humans. NDV is recognized as an oncolytic virus, and its use in humans for oncological treatment is being evaluated.In the present work, we have developed two types of NDV-vectored candidate vaccines, which carry the surface-exposed RBD and S1 antigens of SARS-CoV-2, respectively. These vaccine candidates were produced in specific-pathogen-free embryonating chicken eggs, and purified from allantoic fluid before lyophilization. These vaccines were administered intranasally to three different animal models: mice, rats and hamsters, and evaluated for safety, toxicity, immunogenicity, stability and efficacy. Efficacy was evaluated in a challenge assay against active SARS-CoV-2 virus in the Golden Syrian hamster model.The NDV-vectored vaccine based on the S1 antigen was shown to be safe and highly immunogenic, with the ability to neutralize SARS-CoV-2 in-vitro, even with an extreme dilution of 1/640. Our results reveal that this vaccine candidate protects the lungs of the animals, preventing cellular damage in this tissue. In addition, this vaccine reduces the viral load in the lungs, suggesting that it may significantly reduce the likelihood of transmission. Being lyophilized, this vaccine candidate is very stable and can be stored for several months at 4-8⁰C.In conclusion, our NDV-based vaccine candidate has shown a very favorable performance in the pre-clinical study, serving as evidence for a future evaluation in a Phase-I human clinical trial. This candidate represents a promising tool in the fight against COVID-19.

scholarly journals Newcastle disease virus (NDV) expressing the spike protein of SARS-CoV-2 as a live virus vaccine candidate

EBioMedicine ◽  
2020 ◽  
Vol 62 ◽  
pp. 103132
Author(s):  
Weina Sun ◽  
Sarah R. Leist ◽  
Stephen McCroskery ◽  
Yonghong Liu ◽  
Stefan Slamanig ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Virus Vaccine ◽  
Newcastle Disease ◽  
Vaccine Candidate ◽  
Spike Protein ◽  
Live Virus ◽  
Live Virus Vaccine

scholarly journals Complete Genome Sequence of Heat-Resistant Newcastle Disease Virus Strain HR09

2017 ◽  
Vol 5 (44) ◽  
Author(s):  
Yongzhong Cao ◽  
Qian Liu ◽  
Xiaorong Zhang ◽  
Hai Hu ◽  
Yantao Wu
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Genome Sequence ◽  
Virus Strain ◽  
Newcastle Disease ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genomic Information ◽  
Heat Resistant ◽  
Complete Genomic

ABSTRACT In this study, the complete genome sequence of the heat-resistant Newcastle disease virus (NDV) strain HR09, which was isolated in China in the 1990s, was determined and characterized phylogenetically. This is the first report regarding the complete genomic information of a thermostable NDV strain from China.

scholarly journals Newcastle Disease Virus Vectored Chicken Infectious Anaemia Vaccine Induces Robust Immune Response in Chickens

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1985
Author(s):  
Madhan Mohan Chellappa ◽  
Sohini Dey ◽  
Dinesh Chandra Pathak ◽  
Asmita Singh ◽  
Narayan Ramamurthy ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Virus ◽  
Vaccine Candidate ◽  
Transcriptional Unit ◽  
Slight Reduction ◽  
Bivalent Vaccine ◽  
Mouth Disease Virus ◽  
Significant Difference

Newcastle disease virus (NDV) strain R2B, with an altered fusion protein cleavage site, was used as a viral vector to deliver the immunogenic genes VP2 and VP1 of chicken infectious anaemia virus (CIAV) to generate a bivalent vaccine candidate against these diseases in chickens. The immunogenic genes of CIAV were expressed as a single transcriptional unit from the NDV backbone and the two CIA viral proteins were obtained as separate entities using a self-cleaving foot-and-mouth disease virus 2A protease sequence between them. The recombinant virus (rR2B-FPCS-CAV) had similar growth kinetics as that of the parent recombinant virus (rR2B-FPCS) in vitro with similar pathogenicity characteristics. The bivalent vaccine candidate when given in specific pathogen-free chickens as primary and booster doses was able to elicit robust humoral and cell-mediated immune (CMI) responses obtained in a vaccination study that was conducted over a period of 15 weeks. In an NDV and CIAV ELISA trial, there was a significant difference in the titres of antibody between vaccinated and control groups which showed slight reduction in antibody titre by 56 days of age. Hence, a second booster was administered and the antibody titres were maintained until 84 days of age. Similar trends were noticed in CMI response carried out by lymphocyte transformation test, CD4+ and CD8+ response by flow cytometry analysis and response of real time PCR analysis of cytokine genes. Birds were challenged with virulent NDV and CIAV at 84 days and there was significant reduction in the NDV shed on the 2nd and 4th days post challenge in vaccinated birds as compared to unvaccinated controls. Haematological parameters comprising PCV, TLC, PLC and PHC were estimated in birds that were challenged with CIAV that indicated a significant reduction in the blood parameters of controls. Our findings support the development and assessment of a bivalent vaccine candidate against NDV and CIAV in chickens.

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