Seasonal population changes in the ammonia-oxidizing bacteria community structure of Songhua Lake, China

2016 ◽  
Vol 104 ◽  
pp. 523-530 ◽  
Author(s):  
Xinyu Zhao ◽  
Yue Zhao ◽  
Beidou Xi ◽  
Zimin Wei ◽  
Junqiu Wu ◽  
...  
Keyword(s):  
Community Structure ◽  
Ammonia Oxidizing Bacteria ◽  
Population Changes ◽  
Bacteria Community Structure ◽  
Seasonal Population

scholarly journals Higher Sensitivity of Soil Microbial Network Than Community Structure under Acid Rain

2021 ◽  
Vol 9 (1) ◽  
pp. 118
Author(s):  
Ziqiang Liu ◽  
Hui Wei ◽  
Jiaen Zhang ◽  
Muhammad Saleem ◽  
Yanan He ◽  
...  
Keyword(s):  
Community Structure ◽  
Acid Rain ◽  
Natural Soil ◽  
Available Phosphorus ◽  
Global Changes ◽  
N2o Emissions ◽  
Ammonia Oxidizing Bacteria ◽  
Soil Core ◽  
N Cycle ◽  
Microbial Network

Acid rain (AR), as a global environmental threat, has profoundly adverse effects on natural soil ecosystems. Microorganisms involved in the nitrogen (N) cycle regulate the global N balance and climate stabilization, but little is known whether and how AR influences the structure and complexity of these microbial communities. Herein, we conducted an intact soil core experiment by manipulating the acidity of simulated rain (pH 7.5 (control, CK) vs. pH 4.0 (AR)) in subtropical agricultural soil, to reveal the differences in the structure and complexity of soil nitrifying and denitrifying microbiota using Illumina amplicon sequencing of functional genes (amoA, nirS, and nosZ). Networks of ammonia-oxidizing archaea (AOA) and nirS-carrying denitrifiers in AR treatment were less complex with fewer nodes and lower connectivity, while network of nosZ-carrying denitrifiers in AR treatment had higher complexity and connectivity relative to CK. Supporting this, AR reduced the abundance of keystone taxa in networks of AOA and nirS-carrying denitrifiers, but increased the abundance of keystone taxa in nosZ-carrying denitrifiers network. However, AR did not alter the community structure of AOA, ammonia-oxidizing bacteria (AOB), nirS-, and nosZ-carrying denitrifiers. Moreover, AR did not change soil N2O emissions during the experimental period. AOB community structure significantly correlated with content of soil available phosphorus (P), while the community structures of nirS- and nosZ-carrying denitrifiers both correlated with soil pH and available P content. Soil N2O emission was mainly driven by the nirS-carrying denitrifiers. Our results present new perspective on the impacts of AR on soil N-cycle microbial network complexity and keystone taxa in the context of global changes.

scholarly journals Soil type determines the abundance and community structure of ammonia-oxidizing bacteria and archaea in flooded paddy soils

2010 ◽  
Vol 10 (8) ◽  
pp. 1510-1516 ◽  
Author(s):  
Xin Chen ◽  
Li-Mei Zhang ◽  
Ju-Pei Shen ◽  
Zhihong Xu ◽  
Ji-Zheng He
Keyword(s):  
Community Structure ◽  
Soil Type ◽  
Paddy Soils ◽  
Ammonia Oxidizing Bacteria

Distribution and Seasonal Population Changes of Campecopea Hirsuta [Isopoda: Flabellifera]

1968 ◽  
Vol 48 (3) ◽  
pp. 761-767 ◽  
Author(s):  
Clare E. Harvey
Keyword(s):  
Life Span ◽  
Late Summer ◽  
Wave Action ◽  
Maximum Length ◽  
Population Changes ◽  
South West ◽  
Steep Slopes ◽  
Seasonal Population

Campecopea hirsuta (Montagu) occurs in rock crevices, and among barnacles and the lichen Lichina in south-west Britain. It is particularly abundant on shores ex-posed to wave action, occurring most commonly at about M.T.L. and below on shallow slopes, and from M.T.L. to H.W.N. on steep slopes. Young Campecopea of about 1–5 mm long are released in late summer. Females attain a maximum length of 35 mm and males 4–0 mm, each having a life span of no more than 1½ years.

scholarly journals Effects of tobacco–peanut relay intercropping on soil bacteria community structure

2019 ◽  
Vol 69 (13) ◽  
pp. 1531-1536 ◽  
Author(s):  
Lin Gao ◽  
Xin-min Liu ◽  
Yong-mei Du ◽  
Hao Zong ◽  
Guo-ming Shen
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Soil Bacteria ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Continuous Cropping ◽  
Soil Microbial ◽  
Relay Intercropping ◽  
Bacteria Community Structure

Abstract Purpose A reasonable cultivation pattern is beneficial to maintain soil microbial activity and optimize the structure of the soil microbial community. To determine the effect of tobacco−peanut (Nicotiana tabacum−Arachis hypogaea) relay intercropping on the microbial community structure in soil, we compared the effects of relay intercropping and continuous cropping on the soil bacteria community structure. Methods We collected soil samples from three different cropping patterns and analyzed microbial community structure and diversity using high-throughput sequencing technology. Result The number of operational taxonomic units (OTU) for bacterial species in the soil was maximal under continuous peanut cropping. At the phylum level, the main bacteria identified in soil were Proteobacteria, Actinobacteria, and Acidobacteria, which accounted for approximately 70% of the total. The proportions of Actinobacteria and Firmicutes increased, whereas the proportion of Proteobacteria decreased in soil with tobacco–peanut relay intercropping. Moreover, the proportions of Firmicutes and Proteobacteria among the soil bacteria further shifted over time with tobacco–peanut relay intercropping. At the genus level, the proportions of Bacillus and Lactococcus increased in soil with tobacco–peanut relay intercropping. Conclusion The community structure of soil bacteria differed considerably with tobacco–peanut relay intercropping from that detected under peanut continuous cropping, and the proportions of beneficial bacteria (the phyla Actinobacteria and Firmicutes, and the genera Bacillus and Lactococcus) increased while the proportion of potentially pathogenic bacteria (the genera Variibacter and Burkholderia) decreased. These results provide a basis for adopting tobacco–peanut relay intercropping to improve soil ecology and microorganisms, while making better use of limited cultivable land.

scholarly journals The Isotope Array, a New Tool That Employs Substrate-Mediated Labeling of rRNA for Determination of Microbial Community Structure and Function

2003 ◽  
Vol 69 (11) ◽  
pp. 6875-6887 ◽  
Author(s):  
Justyna Adamczyk ◽  
Martin Hesselsoe ◽  
Niels Iversen ◽  
Matthias Horn ◽  
Angelika Lehner ◽  
...  
Keyword(s):  
Community Structure ◽  
Activated Sludge ◽  
Fluorescence Labeling ◽  
Specific Substrate ◽  
Ammonia Oxidizing Bacteria ◽  
Microarray Hybridization ◽  
Independent Manner ◽  
And Function

ABSTRACT A new microarray method, the isotope array approach, for identifying microorganisms which consume a 14C-labeled substrate within complex microbial communities was developed. Experiments were performed with a small microarray consisting of oligonucleotide probes targeting the 16S rRNA of ammonia-oxidizing bacteria (AOB). Total RNA was extracted from a pure culture of Nitrosomonas eutropha grown in the presence of [14C]bicarbonate. After fluorescence labeling of the RNA and microarray hybridization, scanning of all probe spots for fluorescence and radioactivity revealed that specific signals were obtained and that the incorporation of 14C into rRNA could be detected unambiguously. Subsequently, we were able to demonstrate the suitability of the isotope array approach for monitoring community composition and CO2 fixation activity of AOB in two nitrifying activated-sludge samples which were incubated with [14C]bicarbonate for up to 26 h. AOB community structure in the activated-sludge samples, as predicted by the microarray hybridization pattern, was confirmed by quantitative fluorescence in situ hybridization (FISH) and comparative amoA sequence analyses. CO2 fixation activities of the AOB populations within the complex activated-sludge communities were detectable on the microarray by 14C incorporation and were confirmed independently by combining FISH and microautoradiography. AOB rRNA from activated sludge incubated with radioactive bicarbonate in the presence of allylthiourea as an inhibitor of AOB activity showed no incorporation of 14C and thus was not detectable on the radioactivity scans of the microarray. These results suggest that the isotope array can be used in a PCR-independent manner to exploit the high parallelism and discriminatory power of microarrays for the direct identification of microorganisms which consume a specific substrate in the environment.

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