Phosphate removal using aerobic bacterial consortium and pure cultures isolated from activated sludge

2015 ◽  
Vol 95 ◽  
pp. 237-246 ◽  
Author(s):  
Leyla Benammar ◽  
Taha Menasria ◽  
Ammar Ayachi ◽  
Messaoud Benounis
Keyword(s):  
Activated Sludge ◽  
Bacterial Consortium ◽  
Phosphate Removal ◽  
Pure Cultures

Interaction of Different Groups of Micro-Organisms in Biological Phosphate Removal

1985 ◽  
Vol 17 (11-12) ◽  
pp. 89-97 ◽  
Author(s):  
Karin E. U. Brodisch
Keyword(s):  
Activated Sludge ◽  
Nutrient Removal ◽  
Continuous Flow ◽  
Phosphate Uptake ◽  
Phosphate Removal ◽  
Batch Experiments ◽  
Flow Conditions ◽  
End Products ◽  
Pure Cultures ◽  
Micro Organisms

Observations under continuous flow conditions in a bench-scale activated sludge unit and in full-scale nutrient removal activated sludge plants indicated that, despite relatively high numbers of Aoinetobaoter oalooaoetious, no enhanced biological phosphate removal was achieved. Total bacteriological analyses of the mixed liquor of these plants indicated that in all cases Aeromonas spp. were present in low numbers, particularly Aeromonas punotata. This bacterium is described as being able to produce acetate as one of its major fermentation end products. Phosphate uptake batch experiments using pure cultures showed that Aeromonas punotata enhances the phosphate uptake capability of Aoinetobaoter calooaoetious by converting carbohydrates present in the sewage to a substrate required for phosphate removal. It was shown that the anaerobic zone in a phosphate removing activated sludge plant is a fermentation reactor, where suitable substrates are generated rather than stress conditions imposed on the bacteria, a condition which was previously believed to be essential for enhanced phosphate uptake.

OPTIMIZATION OF PHOSPHATE REMOVAL FROM SYNTHETIC WASTEWATER BY BACTERIAL CONSORTIUM USING BOX-BEHNKEN DESIGN

2013 ◽  
Vol 12 (12) ◽  
pp. 2371-2383
Author(s):  
Krishnaswamy Usharani ◽  
Perumalsamy Lakshmanaperumalsamy ◽  
Muthusamy Muthukumar
Keyword(s):  
Bacterial Consortium ◽  
Phosphate Removal ◽  
Synthetic Wastewater ◽  
Box Behnken Design

Operational Results of the Wolfsburg Wastewater Treatment Plant

1992 ◽  
Vol 25 (4-5) ◽  
pp. 203-209 ◽  
Author(s):  
R. Kayser ◽  
G. Stobbe ◽  
M. Werner
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Content ◽  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Phosphate Removal ◽  
Total Nitrogen Content ◽  
Activated Sludge Process

At Wolfsburg for a load of 100,000 p.e., the step-feed activated sludge process for nitrogen removal is successfully in operation. Due to the high denitrification potential (BOD:TKN = 5:1) the effluent total nitrogen content can be kept below 10 mg l−1 N; furthermore by some enhanced biological phosphate removal about 80% phosphorus may be removed without any chemicals.

Observations Supporting Phosphate Removal by Biological Excess Uptake – A Review

1983 ◽  
Vol 15 (3-4) ◽  
pp. 15-41 ◽  
Author(s):  
G v R Marais ◽  
R E Loewenthal ◽  
I P Siebritz
Keyword(s):  
Activated Sludge ◽  
P Uptake ◽  
Phosphate Removal ◽  
Activated Sludge Process ◽  
Biochemical Mechanism ◽  
Paper Briefly ◽  
Uptake And Release

The paper briefly reviews the development of the biological excess removal of phosphorus in the activated sludge process, from 1959 when it was first observed to the present. It concludes by proposing, tentatively, a biochemical mechanism whereby excess P uptake and release can be explained.

Triclosan in contact with activated sludge and its impact on phosphate removal and microbial community

2021 ◽  
Vol 319 ◽  
pp. 124134
Author(s):  
Xiaoqi Dong ◽  
Yuzhe He ◽  
Xingxing Peng ◽  
Xiaoshan Jia
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Phosphate Removal

The Influence of Nitrogen on Phosphorus Removal in Activated Sludge Plants

1982 ◽  
Vol 14 (1-2) ◽  
pp. 31-45 ◽  
Author(s):  
J L Barnard
Keyword(s):  
Organic Carbon ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Complete Removal ◽  
Phosphate Removal ◽  
Electron Donors ◽  
Biological Phosphorus Removal ◽  
Anaerobic Conditions ◽  
Low Level ◽  
Biological Phosphorus

This paper discusses the need for anaerobiases as a pre-requisite for phosphorus removal in activated sludge plants and the effect of nitrates on the anaerobic conditions. If the plant could be operated to avoid nitrification, biological phosphorus removal presents no problems. When nitrification is required, the nitrates must be reduced to a low level through internal denitrification. If sufficient carbon is available to ensure complete removal of the nitrates and anaerobic conditions in a specific zone in the plant, good phosphate removal can be ensured. Below COD : TKN ratios of 10 : 1 it is becoming more difficult to control the plant and special care should be taken to determine not only the quantity of organic carbon available as electron donors for removal of the nitrates but also the form in which it arrives at the plant.

scholarly journals Molecular Basis of a Bacterial Consortium: Interspecies Catabolism of Atrazine

1998 ◽  
Vol 64 (1) ◽  
pp. 178-184 ◽  
Author(s):  
Mervyn L. de Souza ◽  
David Newcombe ◽  
Sam Alvey ◽  
David E. Crowley ◽  
Anthony Hay ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Southern Hybridization ◽  
Cyanuric Acid ◽  
Bacterial Species ◽  
Bacterial Consortium ◽  
Triazine Ring ◽  
Carbon And Nitrogen ◽  
Consortium Member ◽  
Ring Compounds ◽  
Pure Cultures

ABSTRACT Pseudomonas sp. strain ADP contains the genes,atzA, -B, and -C, that encode three enzymes which metabolize atrazine to cyanuric acid. Atrazine-catabolizing pure cultures isolated from around the world contain genes homologous to atzA, -B, and -C. The present study was conducted to determine whether the same genes are present in an atrazine-catabolizing bacterial consortium and how the genes and metabolism are subdivided among member species. The consortium contained four or more bacterial species, but two members, Clavibacter michiganese ATZ1 andPseudomonas sp. strain CN1, collectively mineralized atrazine. C. michiganese ATZ1 released chloride from atrazine, produced hydroxyatrazine, and contained a homolog to theatzA gene that encoded atrazine chlorohydrolase. C. michiganese ATZ1 stoichiometrically metabolized hydroxyatrazine to N-ethylammelide and contained genes homologous toatzB and atzC, suggesting that either a functional AtzB or -C catalyzed N-isopropylamine release from hydroxyatrazine. C. michiganese ATZ1 grew on isopropylamine as its sole carbon and nitrogen source, explaining the ability of the consortium to use atrazine as the sole carbon and nitrogen source. A second consortium member, Pseudomonassp. strain CN1, metabolized the N-ethylammelide produced byC. michiganese ATZ1 to transiently form cyanuric acid, a reaction catalyzed by AtzC. A gene homologous to the atzCgene of Pseudomonas sp. strain ADP was present, as demonstrated by Southern hybridization and PCR. Pseudomonassp. strain CN1, but not C. michiganese, metabolized cyanuric acid. The consortium metabolized atrazine faster than didC. michiganese individually. Additionally, the consortium metabolized a much broader set of triazine ring compounds than did previously described pure cultures in which the atzABCgenes had been identified. These data begin to elucidate the genetic and metabolic bases of catabolism by multimember consortia.

Comparison of hydrolytic enzyme systems in pure culture and activated sludge under different electron acceptor conditions

1998 ◽  
Vol 37 (4-5) ◽  
pp. 335-343 ◽  
Author(s):  
Rajeev Goel ◽  
Takashi Mino ◽  
Hiroyasu Satoh ◽  
Tomonori Matsuo
Keyword(s):  
Enzyme Activity ◽  
Activated Sludge ◽  
Electron Acceptor ◽  
Bacillus Amyloliquefaciens ◽  
Enzyme System ◽  
Weak Link ◽  
Batch Reactor ◽  
Hydrolytic Enzyme ◽  
Hydrolytic Enzymes ◽  
Pure Cultures

Enzymatic hydrolysis under different electron acceptor conditions in nutrient removal activated sludge treatment processes is a weak link in the Activated Sludge Model no. 2 (Henze et al., 1995). An experimental study was undertaken to gain insight into the hydrolysis process with specific focus on hydrolysis kinetics and rates under different electron acceptor conditions. Two pure cultures, Bacillus amyloliquefaciens (Gram positive) and Pseudomonas saccharophila (Gram negative) were chosen for the study. In addition, activated sludge grown in an anaerobic-aerobic system was tested for enzymatic activity using starch as the model substrate. The hydrolytic enzymes were found to be released into the bulk in pure cultures whereas the enzyme activity was found to be mainly associated with the cell surfaces in activated sludge. Further, it was observed that the development of the hydrolytic enzyme system in Bacillus amyloliquefaciens and P. saccharophila is strongly suppressed under anoxic and anaerobic conditions. However, the effect of anaerobic and aerobic incubation on hydrolytic enzyme activity in activated sludge was found to be small. Starch hydrolysis kinetic data from batch experiments with activated sludge followed substrate saturation kinetics that were linear with biomass concentration. Finally, the similar hydrolytic enzyme activities observed under anaerobic and aerobic phases of a sequencing batch reactor are explained by considering the aspects of enzyme location and enzyme system development under aerobic and anaerobic phases. It is proposed that the floc bound enzymes are recycled in a single sludge system so that an equilibrium exists between enzyme loss and synthesis at steady state.

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