Establishment of an organotypic in vitro culture system and its relevance to the characterization of human prostate epithelial cancer cells and their stromal interactions

2007 ◽  
Vol 203 (4) ◽  
pp. 209-216 ◽  
Author(s):  
Sandra Papini ◽  
Alfredo Rosellini ◽  
Anna De Matteis ◽  
Daniela Campani ◽  
Cesare Selli ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Cancer Cells ◽  
Culture System ◽  
Human Prostate ◽  
Epithelial Cancer ◽  
In Vitro Culture System

scholarly journals In-vitro culture system for mesenchymal progenitor cells derived from waste human ovarian follicular fluid

2014 ◽  
Vol 29 (4) ◽  
pp. 457-469 ◽  
Author(s):  
Federica Riva ◽  
Claudia Omes ◽  
Roberto Bassani ◽  
Rossella E Nappi ◽  
Giuliano Mazzini ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Progenitor Cells ◽  
Follicular Fluid ◽  
Culture System ◽  
Mesenchymal Progenitor Cells ◽  
In Vitro Culture System ◽  
Ovarian Follicular Fluid

First description of an in vitro culture system for Eimeria ovinoidalis macromeront formation in primary host endothelial cells

2016 ◽  
Vol 65 (5) ◽  
pp. 516-519 ◽  
Author(s):  
Tessa Carrau ◽  
Liliana Machado Ribeiro Silva ◽  
David Pérez ◽  
Rocio Ruiz de Ybáñez ◽  
Anja Taubert ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
In Vitro Culture ◽  
Culture System ◽  
Primary Host ◽  
In Vitro Culture System

scholarly journals Establishment of in vitro culture system for Codonopsis pilosula transgenic hairy roots

3 Biotech ◽  
2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Jing Yang ◽  
Xiaozeng Yang ◽  
Bin Li ◽  
Xiayang Lu ◽  
Jiefang Kang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Hairy Roots ◽  
Culture System ◽  
Codonopsis Pilosula ◽  
In Vitro Culture System ◽  
Transgenic Hairy Roots

Long-Term Ex Vivo Maintenance and Expansion of Transplantable Human Hematopoietic Stem Cells

Blood ◽  
1999 ◽  
Vol 94 (5) ◽  
pp. 1623-1636 ◽  
Author(s):  
Chu-Chih Shih ◽  
Mickey C.-T. Hu ◽  
Jun Hu ◽  
Jeffrey Medeiros ◽  
Stephen J. Forman
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
In Vitro Culture ◽  
Culture System ◽  
Ex Vivo ◽  
Human Stem Cells ◽  
Hematopoietic Stem ◽  
In Vitro Culture System

Abstract We have developed a stromal-based in vitro culture system that facilitates ex vivo expansion of transplantable CD34+thy-1+ cells using long-term hematopoietic reconstitution in severe combined immunodeficient-human (SCID-hu) mice as an in vivo assay for transplantable human hematopoietic stem cells (HSCs). The addition of leukemia inhibitory factor (LIF) to purified CD34+ thy-1+ cells on AC6.21 stroma, a murine bone marrow–derived stromal cell line, caused expansion of cells with CD34+ thy-1+ phenotype. Addition of other cytokines, including interleukin-3 (IL-3), IL-6, granulocyte-macrophage colony-stimulating factor, and stem cell factor, to LIF in the cultures caused a 150-fold expansion of cells retaining the CD34+ thy-1+ phenotype. The ex vivo–expanded CD34+ thy-1+ cells gave rise to multilineage differentiation, including myeloid, T, and B cells, when transplanted into SCID-hu mice. Both murine LIF (cannot bind to human LIF receptor) and human LIF caused expansion of human CD34+ thy-1+ cells in vitro, suggesting action through the murine stroma. Furthermore, another human HSC candidate, CD34+ CD38− cells, shows a similar pattern of proliferative response. This suggests thatex vivo expansion of transplantable human stem cells under this in vitro culture system is a general phenomenon and not just specific for CD34+ thy-1+ cells.

scholarly journals Efficient and simple approach to in vitro culture of primary epithelial cancer cells

2016 ◽  
Vol 36 (6) ◽  
Author(s):  
Karolina Janik ◽  
Marta Popeda ◽  
Joanna Peciak ◽  
Kamila Rosiak ◽  
Maciej Smolarz ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
In Vitro Culture ◽  
Cancer Cells ◽  
Simple Approach ◽  
Primary Cell ◽  
Epithelial Cancer ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Cell Medium

Primary breast and prostate epithelial cancer cells may be efficiently cultured in vitro using simple and easily validatable approach–plates coated with a mixture of extracellular matrix components and tissue-specific primary cell medium.

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