Molecular Characterization of Iron-Containing Superoxide Dismutases in the Heterotrophic Dinoflagellate Crypthecodinium cohnii

Protist ◽  
2008 ◽  
Vol 159 (2) ◽  
pp. 223-238 ◽  
Author(s):  
Fabienne Dufernez ◽  
Evelyne Derelle ◽  
Christophe Noël ◽  
Giovanna Sanciu ◽  
Cléa Mantini ◽  
...  
2004 ◽  
Vol 37 (8) ◽  
pp. 1170-1181 ◽  
Author(s):  
Amandine Plantivaux ◽  
Paola Furla ◽  
Didier Zoccola ◽  
Ginette Garello ◽  
Didier Forcioli ◽  
...  

Gene ◽  
2007 ◽  
Vol 387 (1-2) ◽  
pp. 93-108 ◽  
Author(s):  
Bhagirathi Dash ◽  
Richard Metz ◽  
Henry J. Huebner ◽  
Weston Porter ◽  
Timothy D. Phillips

2008 ◽  
Vol 7 (5) ◽  
pp. 872-880 ◽  
Author(s):  
Philippe Deschamps ◽  
Delphine Guillebeault ◽  
Jimi Devassine ◽  
David Dauvillée ◽  
Sophie Haebel ◽  
...  

ABSTRACT The nature of the cytoplasmic pathway of starch biosynthesis was investigated in the model heterotrophic dinoflagellate Crypthecodinium cohnii. The storage polysaccharide granules were shown to be composed of both amylose and amylopectin fractions with a chain length distribution and crystalline organization very similar to those of green algae and land plant starch. Preliminary characterization of the starch pathway demonstrated that C. cohnii contains multiple forms of soluble starch synthases and one major 110-kDa granule-bound starch synthase. All purified enzymes displayed a marked substrate preference for UDP-glucose. At variance with most other microorganisms, the accumulation of starch in the dinoflagellate occurs during early and mid-log phase, with little or no synthesis witnessed when approaching stationary phase. In order to establish a genetic system allowing the study of cytoplasmic starch metabolism in eukaryotes, we describe the isolation of marker mutations and the successful selection of random recombinant populations after homothallic crosses.


2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom

2012 ◽  
Vol 224 (03) ◽  
Author(s):  
A Streltsov ◽  
S Emmrich ◽  
F Engeland ◽  
JH Klusmann

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