scholarly journals Characterization of cookies formulated with rice and black bean extruded flours

2011 ◽  
Vol 1 ◽  
pp. 1645-1652 ◽  
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Priscila Z. Bassinello ◽  
Daniela de G.C. Freitas ◽  
José Luiz R. Ascheri ◽  
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M. R. Costa ◽  
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Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


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