scholarly journals Decolorization of Remazol Brilliant Blue R by New Isolated White Rot Fungus Collected from Tropical Rain Forest in East Kalimantan and its Ligninolytic Enzymes Activity

2015 ◽  
Vol 28 ◽  
pp. 45-51 ◽  
Author(s):  
Tito Sumandono ◽  
Henderson Saragih ◽  
Migirin ◽  
Takashi Watanabe ◽  
Rudianto Amirta
Keyword(s):  
Rain Forest ◽  
Tropical Rain Forest ◽  
Ligninolytic Enzymes ◽  
White Rot ◽  
Brilliant Blue ◽  
White Rot Fungus ◽  
Enzymes Activity ◽  
Remazol Brilliant Blue R ◽  
East Kalimantan

scholarly journals Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3914 ◽  
Author(s):  
Peng Qin ◽  
Yuetong Wu ◽  
Bilal Adil ◽  
Jie Wang ◽  
Yunfu Gu ◽  
...  
Keyword(s):  
Ganoderma Lucidum ◽  
Reactive Dye ◽  
White Rot ◽  
Brilliant Blue ◽  
White Rot Fungus ◽  
Protein Mass ◽  
Remazol Brilliant Blue R ◽  
Protein Mass Spectrometry ◽  
Laccase Enzyme

Many dyes and pigments are used in textile and printing industries, and their wastewater has been classed as a top source of pollution. Biodegradation of dyes by fungal laccase has great potential. In this work, the influence of reaction time, pH, temperature, dye concentration, metal ions, and mediators on laccase-catalyzed Remazol Brilliant Blue R dye (RBBR) decolorization were investigated in vitro using crude laccase from the white-rot fungus Ganoderma lucidum. The optimal decolorization percentage (50.3%) was achieved at 35 °C, pH 4.0, and 200 ppm RBBR in 30 min. The mediator effects from syringaldehyde, 1-hydroxybenzotriazole, and vanillin were compared, and 0.1 mM vanillin was found to obviously increase the decolorization percentage of RBBR to 98.7%. Laccase-mediated decolorization percentages significantly increased in the presence of 5 mM Na+ and Cu2+, and decolorization percentages reached 62.4% and 62.2%, respectively. Real-time fluorescence-quantitative PCR (RT-PCR) and protein mass spectrometry results showed that among the 15 laccase isoenzyme genes, Glac1 was the main laccase-contributing gene, contributing the most to the laccase enzyme activity and decolorization process. These results also indicate that under optimal conditions, G. lucidum laccases, especially Glac1, have a strong potential to remove RBBR from reactive dye effluent.

Decolorization of Orange G and Remazol Brilliant Blue R by the white rot fungus Dichomitus squalens: Toxicological evaluation and morphological study

Chemosphere ◽  
2007 ◽  
Vol 69 (5) ◽  
pp. 795-802 ◽  
Author(s):  
Ivana Eichlerová ◽  
Ladislav Homolka ◽  
Oldřich Benada ◽  
Olga Kofroňová ◽  
Tomáš Hubálek ◽  
...  
Keyword(s):  
Morphological Study ◽  
White Rot ◽  
Brilliant Blue ◽  
White Rot Fungus ◽  
Toxicological Evaluation ◽  
Remazol Brilliant Blue R ◽  
Dichomitus Squalens ◽  
Orange G

scholarly journals Aktivitas ligninolitik Omphalina sp. hasil isolasi dari TKKS dan aplikasinya untuk dekolorisasi limbah kosmetik Ligninolytic activity of Omphalina sp. isolated from EFB and its application for decolorization of cosmetic waste

2016 ◽  
Vol 80 (2) ◽  
Author(s):  
. SUHARYANTO ◽  
Irma KRESNAWATY ◽  
Haryo Tejo PRAKOSO ◽  
Deden Dewantara ERIS
Keyword(s):  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot ◽  
Brilliant Blue ◽  
Optimum Dose ◽  
Optimum Conditions ◽  
Ligninolytic Activity ◽  
Remazol Brilliant Blue R ◽  
Peroxidase Enzyme ◽  
Oxidation System

Abstract White-rot fungi (WRF) are belong to Basidiomycetes group that capable to degrade lignin, because they produce extracelullar ligninolytic enzymes such as lignin peroxsidase (Li-P), mangan peroxidase (Mn-P) and laccase. The ligninolytic activity can be used in bioprocess oxidation system such as biopulping, biobleaching and bioremediation.  The purposes of this research were to determine the optimum conditions of growth and ligni-nolytic activity of  Omphalina and to observe its potential to decolorize cosmetics wastewater.  Omphalina sp. was grown on media of PDA-Remazol Brilliant Blue R (RBBR) and PDA-Guaiacol (GU) at various pH and temperature conditions. The decolorization of cosmetic effluent was conducted by applying Omphalina sp. at various dose of inoculum.  Decolorization rate and change of COD were observed for eight days. The  results  showed that Ompha-lina sp. could grow and produce peroxidase enzyme both on RBBR and GU media at pH 4.5-8.5  and temperature 23-350C. Optimum dose of inoculum was as much as 5%  w/v at which the fungus was able to  decolorize cosmetic factory effluent up to 92.79% and to decrease COD value up to  48.57 % after eight days of incubation.Abstrak Jamur pelapuk putih (JPP) merupakan jamur kelompok Basidiomycetes yang mampu mendegradasi lignin karena memproduksi enzim-enzim ligninolitik ekstraseluler seperti lignin peroksidase (Li-P), mangan peroksidase (Mn-P) dan lakase.  Kemampuan ligninolitik JPP dapat dimanfaatkan dalam sistem oksidasi bioproses seperti biopulping, biobleaching dan bioremediasi. Pene-litian bertujuan menetapkan kondisi optimum pertumbuhan Omphalina sp. dan aktivitas ligninolitik yang dihasilkan-nya serta mempelajari potensinya dalam mendekolorisasi limbah cair kosmetik. Omphalina sp. ditumbuhkan dalam media PDA-Remazol Brilliant Blue R (RBBR) dan PDA-Guaiakol (GU)  pada  berbagai variasi pH dan suhu. Percobaan dekolorisasi limbah cair kosmetik dilakukan dengan aplikasi inokulum dalam berbagai dosis. Laju dekolorisasi dan perubahan COD diamati selama delapan hari. Hasil penelitian menunjukkan Omphalina sp. tumbuh dan menghasilkan enzim peroksidase, baik pada  media RBBR maupun GU pada pH 4,5-8,5 dan suhu 25-350C. Dosis optimum aplikasi Omphalina sp. adalah 5% (b/v) yang mampu mendekolorisasi limbah cair pabrik kosmetik hingga 92,79%  dan menurunkan COD 48,57% setelah delapan hari.

scholarly journals Aktivitas ligninolitik Omphalina sp. hasil isolasi dari TKKS dan aplikasinya untuk dekolorisasi limbah kosmetik Ligninolytic activity of Omphalina sp. isolated from EFB and its application for decolorization of cosmetic waste

2016 ◽  
Vol 80 (2) ◽  
Author(s):  
. SUHARYANTO ◽  
Irma KRESNAWATY ◽  
Haryo Tejo PRAKOSO ◽  
Deden Dewantara ERIS
Keyword(s):  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot ◽  
Brilliant Blue ◽  
Optimum Dose ◽  
Optimum Conditions ◽  
Ligninolytic Activity ◽  
Remazol Brilliant Blue R ◽  
Peroxidase Enzyme ◽  
Oxidation System

Abstract White-rot fungi (WRF) are belong to Basidiomycetes group that capable to degrade lignin, because they produce extracelullar ligninolytic enzymes such as lignin peroxsidase (Li-P), mangan peroxidase (Mn-P) and laccase. The ligninolytic activity can be used in bioprocess oxidation system such as biopulping, biobleaching and bioremediation.  The purposes of this research were to determine the optimum conditions of growth and ligni-nolytic activity of  Omphalina and to observe its potential to decolorize cosmetics wastewater.  Omphalina sp. was grown on media of PDA-Remazol Brilliant Blue R (RBBR) and PDA-Guaiacol (GU) at various pH and temperature conditions. The decolorization of cosmetic effluent was conducted by applying Omphalina sp. at various dose of inoculum.  Decolorization rate and change of COD were observed for eight days. The  results  showed that Ompha-lina sp. could grow and produce peroxidase enzyme both on RBBR and GU media at pH 4.5-8.5  and temperature 23-350C. Optimum dose of inoculum was as much as 5%  w/v at which the fungus was able to  decolorize cosmetic factory effluent up to 92.79% and to decrease COD value up to  48.57 % after eight days of incubation.Abstrak Jamur pelapuk putih (JPP) merupakan jamur kelompok Basidiomycetes yang mampu mendegradasi lignin karena memproduksi enzim-enzim ligninolitik ekstraseluler seperti lignin peroksidase (Li-P), mangan peroksidase (Mn-P) dan lakase.  Kemampuan ligninolitik JPP dapat dimanfaatkan dalam sistem oksidasi bioproses seperti biopulping, biobleaching dan bioremediasi. Pene-litian bertujuan menetapkan kondisi optimum pertumbuhan Omphalina sp. dan aktivitas ligninolitik yang dihasilkan-nya serta mempelajari potensinya dalam mendekolorisasi limbah cair kosmetik. Omphalina sp. ditumbuhkan dalam media PDA-Remazol Brilliant Blue R (RBBR) dan PDA-Guaiakol (GU)  pada  berbagai variasi pH dan suhu. Percobaan dekolorisasi limbah cair kosmetik dilakukan dengan aplikasi inokulum dalam berbagai dosis. Laju dekolorisasi dan perubahan COD diamati selama delapan hari. Hasil penelitian menunjukkan Omphalina sp. tumbuh dan menghasilkan enzim peroksidase, baik pada  media RBBR maupun GU pada pH 4,5-8,5 dan suhu 25-350C. Dosis optimum aplikasi Omphalina sp. adalah 5% (b/v) yang mampu mendekolorisasi limbah cair pabrik kosmetik hingga 92,79%  dan menurunkan COD 48,57% setelah delapan hari.

The estimation of temporal processes in tropical rain forest: a study of primary mixed dipterocarp forest in Indonesia

1985 ◽  
Vol 1 (2) ◽  
pp. 171-182 ◽  
Author(s):  
Soedarsono Riswan ◽  
J. B. Kenworthy ◽  
Kuswata Kartawinata
Keyword(s):  
Time Scale ◽  
Rain Forest ◽  
Tropical Rain Forest ◽  
Gap Formation ◽  
Growth Rings ◽  
Secondary Species ◽  
East Kalimantan ◽  
Forest Sites ◽  
Time Required ◽  
Individual Trees

ABSTRACTIn the absence of growth rings it is difficult to give a precise time scale for processes associated with the re-establishment of tropical rain forest. This paper explores other methods by which a time scale may be constructed. The proportions of primary and secondary species, an index of similarity, biomass measurements, girth dimensions and gap size are all considered from sites in East Kalimantan, Indonesia. Data from primary, secondary and experimentally cleared forest sites are compared to estimate the minimum time required for various phases involved in the re-establishment of tropical rain forest after disturbance. A simple model is proposed to accommodate the data and other estimates in the literature. The model predicts a minimum period for the stablization of secondary species numbers as 60–70 years and the replacement of primary species as 150 years at which point gap formation is initiated. After approximately 220–250 years biomass stabilizes while individual trees exist for over 500 years.

scholarly journals Decolourisation Capabilities of Ligninolytic Enzymes Produced byMarasmius cladophyllusUMAS MS8 on Remazol Brilliant Blue R and Other Azo Dyes

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Ngieng Ngui Sing ◽  
Ahmad Husaini ◽  
Azham Zulkharnain ◽  
Hairul Azman Roslan
Keyword(s):  
Azo Dyes ◽  
Culture Medium ◽  
Laccase Activity ◽  
Dye Degradation ◽  
Ligninolytic Enzymes ◽  
Brilliant Blue ◽  
Remazol Brilliant Blue R ◽  
Orange G ◽  
Dye Decolourisation

Marasmius cladophylluswas examined for its ability to degradatively decolourise the recalcitrant dye Remazol Brilliant Blue R (RBBR) and screened for the production of ligninolytic enzymes using specific substrates. Monitoring dye decolourisation by the decrease in absorbance ratio ofA592/A500shows that the decolourisation of RBBR dye was associated with the dye degradation.Marasmius cladophyllusproduces laccase and lignin peroxidase in glucose minimal liquid medium containing RBBR. Both enzyme activities were increased, with laccase activity recorded 70 times higher reaching up to 390 U L−1on day 12. Further in vitro RBBR dye decolourisation using the culture medium shows that laccase activity was correlated with the dye decolourisation. Fresh RBBR dye continuously supplemented into the decolourised culture medium was further decolourised much faster in the subsequent round of the RBBR dye decolourisation. In vitro dye decolourisation using the crude laccase not only decolourised 76% of RBBR dye in just 19 hours but also decolourised 54% of Orange G and 33% of Congo red at the same period of time without the use of any exogenous mediator. This rapid dye decolourisation ability of the enzymes produced byM. cladophyllusthus suggested its possible application in the bioremediation of dye containing wastewater.

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