scholarly journals High-Level Toolset For Comprehensive Visual Data Analysis and Model Validation

2017 ◽  
Vol 108 ◽  
pp. 2090-2099 ◽  
Author(s):  
Konstantin Ryabinin ◽  
Svetlana Chuprina
Author(s):  
Daniel Baum ◽  
Felix Herter ◽  
John Møller Larsen ◽  
Achim Lichtenberger ◽  
Rubina Raja

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Biricik ◽  
V Bianchi ◽  
F Lecciso ◽  
M Surdo ◽  
M Manno ◽  
...  

Abstract Study question To explore ploidy concordance between invasive and non-invasive PGTA (niPGT-A) at different embryo culture time. Summary answer High level (>84%) of concordance rate for ploidy and sex, sensitivity (>88%), and specificity (76%) were obtained for both day6/7 samples and day5 samples. What is known already The analysis of embryo cell free DNA (cfDNA) that are released into culture media during in vitro embryo development has the potential to evaluate embryo ploidy status. However, obtaining sufficient quality and quantity of cfDNA is essential to achieve interpretable results for niPGT-A. More culture time is expected to be directly proportional to the release of more cfDNA. But embryo culture time is limited due to in-vitro embryo survival potential. Therefore, it is important to estimate the duration of the culture that will provide the maximum cfDNA that can be obtained without adversely affecting the development of the embryo. Study design, size, duration A total of 105 spent culture media (SCM) from day5-day7 blastocyst stage embryos have been included in this cohort study. The cfDNA of SCM samples were amplified and analyzed for niPGT-A by NGS analysis. The SCM samples were divided into 2 subgroups according the embryo culture hours (Day5 and Day6/7 group). The DNA concentration, informativity and euploidy results have then been compared with their corresponding embryos after trophectoderm biopsy (TE) and PGT-A analysis by NGS Participants/materials, setting, methods Embryos cultured until Day3 washed and cultured again in 20µl fresh culture media until embryo biopsy on Day5, 6, or 7. After biopsy SCM samples were immediately collected in PCR tubes and conserved at –20 °C until whole genome amplification by MALBAC® (Yicon Genomics). The TE and SCM samples were analyzed by next-generation sequencing (NGS) using Illumina MiSeq® System. NGS data analysis has been done by Bluefuse Multi Software 4.5 (Illumina) for SCM and TE samples Main results and the role of chance Only the SCM samples which have an embryo with a conclusive result were included in this cohort (n = 105). Overall 97.1% (102/105) of SCM samples gave a successful DNA amplification with a concentration ranging 32.4–128.5ng/µl. Non-informative (NI) results including a chaotic profile (>5 chromosome aneuploidies) were observed in 17 samples, so 83.3%(85/102) of SCM samples were informative for NGS data analysis. Ploidy concordance rate with the corresponding TE biopsies (euploid vs euploid, aneuploid vs aneuploid) was 84.7% (72/85). Sensitivity and specificity were 92,8% and 76,7%, respectively with no significant difference for all parameters for day 6/7 samples compared with day 5 samples. The false-negative rate was 3.5% (3/85), and false-positive rate was 11.7% (10/85). Limitations, reasons for caution The sample size is relatively small. Larger prospective studies are needed. As this is a single-center study, the impact of the variations in embryo culture conditions can be underestimated. Maternal DNA contamination risk cannot be revealed in SCM, therefore the use of molecular markers would increase the reliability. Wider implications of the findings: Non-invasive analysis of embryo cfDNA analyzed in spent culture media demonstrates high concordance with TE biopsy results in both early and late culture time. A non-invasive approach for aneuploidy screening offers important advantages such as avoiding invasive embryo biopsy and decreased cost, potentially increasing accessibility for a wider patient population. Trial registration number Not applicable


Author(s):  
Werner Benger ◽  
René Heinzl ◽  
Dietmar Hildenbrand ◽  
Tino Weinkauf ◽  
Holger Theisel ◽  
...  

2018 ◽  
Author(s):  
Dewi Putri Handayani ◽  
Rahadian Zainul ◽  
Fajriah Azra

Abstract— This research is aimed to produce a prezi multimedia based on Problem Based Learning (PBL) in Basic Law of Chemistry material and to determine the level ov validaty and practicality based on media’s function. This research is Researc and Development (R&D) which applies 4-D madel: define, design, develop, and disseminate. The research is done until development stage. Determining the level of validity and practicality are done by Chemistry lecturer, Chemistry teacher,and students of class XI IPA SMAN 1 Bukittinggi. The research instrument is questionnaire which consists of validity questionnaire and practicality questionnaire. The technic of data collection is done by distributing questionnaire. The data is analyzed by using moment kappa. From the data analysis,it is found that Prezi Multimedia based on Problem Based Learning (PBL) in Basic Law of Chemistry material has moment kappa validity about 0,85 with very high level of validity, moment kappa practicality from teacher’s evaluation about 0,86 with very high level of practicality, and moment kappa practicality from student’s evaluation about 0,80 with high level of practicality. The learning media that have been developed is proved valid and practical, so it can be used on Chemistry learning process in class X IPA at SMAN 1 Bukittinggi.


2012 ◽  
Vol 19 (2) ◽  
pp. 5-17
Author(s):  
Bartosz Czaplewski ◽  
Krzysztof Czaplewski

Abstract Vessel traffic systems provide a high level of safety on coastal waters due to coastal radar stations and industrial cameras transmitting information to traffic supervision centers, as well. To improve a vessel traffic services is very important to ensure the speed and secrecy for the transmission of video images. The paper presents the basic issues of the multimedia data protection by digital watermarking and fingerprinting methods. Main applications for such digital marking were described in the paper as well as its requirements. Furthermore, the importance of multicast transmission for fingerprinting methods was presented by comparing the scalability of methods using only unicast transmissions and methods using multicast transmissions. The paper also presents the greatest threat to fingerprinting methods, which are attacks performed by more than one pirate. These attacks are called collusion attacks. The criteria that should be followed during identifying rogue users taking part in the collusion attack on the security systems has been presented. The paper also contains description of the extended Hillcast method, which belongs to the group of JFD (Joint Fingerprinting and Decryption) methods. The method provides a cryptographic security and digital fingerprinting of multimedia content, while maintaining high scalability. Main purpose of this method is VoD (Video on Demand) service, but it can also be used in vessel traffic supervision systems, such as VTS and AIS. In the last part of paper, there are results of studies which indicate high resistance to most common collusion attacks. Method proposed by authors can enhance the security of visual data transmission in vessel traffic systems.


2020 ◽  
Vol 2 (2) ◽  
pp. 70-76
Author(s):  
Mujahidah ◽  
Nur Afiah ◽  
Syaiful

Perception can be interpreted as a process of giving meaning to a phenomenon, event or object. professionalism is interpreted as a professional trait. Those traits are mastering the Science in their field and have high skills in carrying out their duties. This Study aimed to describe the students' perceptions of professionalism lecturer English Education Program, State Islamic Institute of Parepare. To achieve this goal a questionnaire was distributed to 50 students (40% of the population). This Research is a descriptive quantitative study with a survey approach. The data processed using simple tabulation techniques and the data qualitatively interpreted. There are five aspects of the professionalism observed, namely: the ability to speak English, online teaching skills, insights, timeliness and using of online learning application. By the results of data analysis found that, the lecturers of English Education Program, State Islamic Institute of Parepare has a high level of professionalism.


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