scholarly journals Malachite Green and Leuco-Malachite Green Detection in Fish Using Modified Enzyme Biosensor

2016 ◽  
Vol 20 ◽  
pp. 85-89 ◽  
Author(s):  
A.P. Nurul Hidayah ◽  
S. Faridah ◽  
M.S. Nur Azura ◽  
A.R. Gayah ◽  
M. Othman ◽  
...  
2018 ◽  
Vol 35 (8) ◽  
pp. 1484-1496 ◽  
Author(s):  
Marc H. G. Berntssen ◽  
Rita Hannisdal ◽  
Louise Buttle ◽  
Rudolf Hoogenveen ◽  
Marcel Mengelers ◽  
...  

2020 ◽  
Vol 157 ◽  
pp. 104941
Author(s):  
T. Alexandra Ferreira ◽  
Israel S. Ibarra ◽  
M. Luisa S. Silva ◽  
Jose M. Miranda ◽  
Jose A. Rodriguez

2013 ◽  
Vol 96 (5) ◽  
pp. 1152-1157 ◽  
Author(s):  
Dominique Hurtaud-Pessel ◽  
Pierrick Couëdor ◽  
Eric Verdon ◽  
Dawn Dowell

Abstract During the AOAC Annual Meeting held from September 30 to October 3, 2012 in Las Vegas, NV, the Expert Review Panel (ERP) on Veterinary Drug Residues reviewed data for the method for determination of residues of three triphenylmethane dyes and their metabolites (malachite green, leuco malachite green, crystal violet, leuco crystal violet, and brilliant green) in aquaculture products by LC/MS/MS, previously published in the Journal of Chromatography A 1218, 1632–1645 (2006). The method data were reviewed and compared to the standard method performance requirements (SMPRs) found in SMPR 2009.001, published in AOAC's Official Methods of Analysis, 19th Ed. (2012). The ERP determined that the data were acceptable, and the method was approved AOAC Official First Action. The method uses acetonitrile to isolate the analyte from the matrix. Then determination is conducted by LC/MS/MS with positive electrospray ionization. Accuracy ranged from 100.1 to 109.8% for samples fortified at levels of 0.5, 0.75, 1.0, and 2.0 μg/kg. Precision ranged from 2.0 to 10.3% RSD for the intraday samples and 1.9 to 10.6% for the interday samples analyzed over 3 days. The described method is designed to accurately operate in the analytical range from 0.5 to 2 μg/kg, where the minimum required performance limit for laboratories has been fixed in the European Union at 2.0 μg/kg for these banned substances and their metabolites. Upper levels of concentrations (1–100 μg/kg) can be analyzed depending on the different optional calibrations used.


1975 ◽  
Vol 21 (3) ◽  
pp. 362-369 ◽  
Author(s):  
David Alan Ahlquist ◽  
Samuel Schwartz

Abstract Sensitivity, stability, and specificity of the color-producing reaction of hydrogen peroxide with benzidine, leucomalachite green, or o-dianisidine were tested in numerous systems containing hemoglobin and other hemoproteins. Use of urea or low temperature (to -12 °C), or both, was highly beneficial, especially with leuco-malachite green, for which the color reaction was stable, after about 15 min, for longer than 24 h, with a colorless blank. Absorbance was 0.3 at a final hemoglobin concentration of 0.27 mg/liter. Nonspecific color produced by substances such as FeCl3 and ascorbic acid was completely eliminated. Of the three leuco-dyes studied, only benzidine yielded a completely linear calibration curve, but its relative instability and reported carcinogenicity are serious disadvantages. No system tested eliminated completely the known inhibition by plasma of the peroxidase activity of these leuco dyes.


2016 ◽  
Vol 28 (4) ◽  
pp. 793-796
Author(s):  
Mamta Nirmal ◽  
Manish Kumar Rai ◽  
Vindhiya Patel ◽  
Raisa Khatoon ◽  
Kalpana Wani ◽  
...  

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