High-level expression of a glycoside hydrolase family 26 β-mannanase from Aspergillus niger in Pichia pastoris for production of partially hydrolysed fenugreek gum

2021 ◽  
Vol 100 ◽  
pp. 90-97
Author(s):  
Nan-Nan Wang ◽  
Jun Liu ◽  
Yan-Xiao Li ◽  
Jun-Wen Ma ◽  
Qiao-Juan Yan ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Pichia Pastoris ◽  
Glycoside Hydrolase ◽  
Glycoside Hydrolase Family ◽  
High Level Expression ◽  
Fenugreek Gum ◽  
High Level ◽  
Hydrolase Family

scholarly journals Biochemical characterization of a glycoside hydrolase family 43 β-D-galactofuranosidase from the fungus Aspergillus niger

2021 ◽  
Author(s):  
Gregory S Bulmer ◽  
Fang Wei Yuen ◽  
Naimah Begum ◽  
Bethan S Jones ◽  
Sabine S Flitsch ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Glycoside Hydrolase ◽  
Biochemical Characterization ◽  
Maximum Activity ◽  
Glycoside Hydrolase Family ◽  
Enzyme Specificity ◽  
Fungal Enzymes ◽  
Glycoside Hydrolase Family 43 ◽  
Hydrolase Family

β-D-Galactofuranose (Galf) and its polysaccharides are found in bacteria, fungi and protozoa but do not occur in mammalian tissues, and thus represent a specific target for anti-pathogenic drugs. Understanding the enzymatic degradation of these polysaccharides is therefore of great interest, but the identity of fungal enzymes with exclusively galactofuranosidase activity has so far remained elusive. Here we describe the identification and characterization of a galactofuranosidase from the industrially important fungus Aspergillus niger. Phylogenetic analysis of glycoside hydrolase family 43 subfamily 34 (GH43_34) members revealed the occurrence of three distinct clusters and, by comparison with specificities of characterized bacterial members, suggested a basis for prediction of enzyme specificity. Using this rationale, in tandem with molecular docking, we identified a putative β-D-galactofuranosidase from A. niger which was recombinantly expressed in Escherichia coli. The Galf-specific hydrolase, encoded by xynD demonstrates maximum activity at pH 5, 25 °C towards 4-Nitrophenyl-β-galactofuranoside (pNP-βGalf), with a Km of 17.9 ± 1.9 mM and Vmax of 70.6 ± 5.3 μmol min-1. The characterization of this first fungal GH43 galactofuranosidase offers further molecular insight into the degradation of Galf-containing structures and may inform clinical treatments against fungal pathogens.

High-level expression and characterization of a thermophilic β-mannanase from Aspergillus niger in Pichia pastoris

2015 ◽  
Vol 37 (9) ◽  
pp. 1853-1859 ◽  
Author(s):  
Shi Yu ◽  
Zhezhe Li ◽  
Yaping Wang ◽  
Wanping Chen ◽  
Lin Fu ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Pichia Pastoris ◽  
High Level Expression ◽  
High Level

Crystal Structure of Aspergillus niger Isopullulanase, a Member of Glycoside Hydrolase Family 49

2008 ◽  
Vol 376 (1) ◽  
pp. 210-220 ◽  
Author(s):  
Masahiro Mizuno ◽  
Atsushi Koide ◽  
Akihiro Yamamura ◽  
Hiromi Akeboshi ◽  
Hiromi Yoshida ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Aspergillus Niger ◽  
Glycoside Hydrolase ◽  
Glycoside Hydrolase Family ◽  
Hydrolase Family

Identification of the acid/base catalyst of a glycoside hydrolase family 3 (GH3) β-glucosidase from Aspergillus niger ASKU28

2013 ◽  
Vol 1830 (3) ◽  
pp. 2739-2749 ◽  
Author(s):  
Preeyanuch Thongpoo ◽  
Lauren S. McKee ◽  
Ana Catarina Araújo ◽  
Prachumporn T. Kongsaeree ◽  
Harry Brumer
Keyword(s):  
Aspergillus Niger ◽  
Glycoside Hydrolase ◽  
Glycoside Hydrolase Family ◽  
Base Catalyst ◽  
Acid Base ◽  
Hydrolase Family

scholarly journals Biochemical characterization of Aspergillus niger CfcI, a glycoside hydrolase family 18 chitinase that releases monomers during substrate hydrolysis

Microbiology ◽  
2012 ◽  
Vol 158 (8) ◽  
pp. 2168-2179 ◽  
Author(s):  
Jolanda M. van Munster ◽  
Rachel M. van der Kaaij ◽  
Lubbert Dijkhuizen ◽  
Marc J. E. C. van der Maarel
Keyword(s):  
Aspergillus Niger ◽  
Glycoside Hydrolase ◽  
Biochemical Characterization ◽  
Glycoside Hydrolase Family ◽  
Hydrolase Family ◽  
Substrate Hydrolysis
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