Mechanism of gamma-aminobutyric acid (GABA) production by a lactic acid bacterium in yogurt-sake

2018 ◽  
Vol 74 ◽  
pp. 21-27 ◽  
Author(s):  
Taketo Ohmori ◽  
Manaka Tahara ◽  
Toshihisa Ohshima
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Gaba Production

scholarly journals Gamma-aminobutyric acid fermentation with date residue by a lactic acid bacterium, Lactobacillus brevis

2018 ◽  
Vol 125 (3) ◽  
pp. 316-319 ◽  
Author(s):  
Momoko Hasegawa ◽  
Daisuke Yamane ◽  
Kouichi Funato ◽  
Atsushi Yoshida ◽  
Yoshihiro Sambongi
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Lactobacillus Brevis ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Acid Fermentation

scholarly journals Gamma-aminobutyric acid (GABA) production in milk fermented by specific wild lactic acid bacteria strains isolated from artisanal Mexican cheeses

2020 ◽  
Vol 70 (1) ◽  
Author(s):  
Alejandro Santos-Espinosa ◽  
Lilia María Beltrán-Barrientos ◽  
Ricardo Reyes-Díaz ◽  
Miguel Ángel Mazorra-Manzano ◽  
Adrián Hernández-Mendoza ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Gaba Production

scholarly journals ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT DARI PANGAN FERMENTASI CINCALOK SEBAGAI PENGHASIL GAMMA-AMINOBUTYRIC ACID

2021 ◽  
Vol 8 (1) ◽  
pp. 25-32
Author(s):  
Adhitya Naufal Pribadhi ◽  
Endang Kusdiyantini ◽  
Rejeki Siti Ferniah
Keyword(s):  
Lactic Acid ◽  
Silica Gel ◽  
Fermented Food ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Bacterial Isolates ◽  
West Kalimantan ◽  
Gaba Production ◽  
Isolation And Characterization ◽  
Layer Chromatography

Isolation and Characterization of Lactic Acid Bacteria from Fermented Food Cincalok as Producer of Gamma-Aminobutyric Acid Cincalok is a fermented food originating from West Kalimantan. This study aimed to obtain lactic acid bacterial isolates (LAB) capable of producing gamma-aminobutyric acid (GABA), to characterize the LAB isolates obtained, and to obtain GABA by the Thin Layer Chromatography (TLC) method. Bacterial growth and GABA production was carried out by adding 5% MSG and without MSG, and measured spectrophotometrically. In this study, 4 LAB bacterial isolates were obtained which were coded CIN-1, CIN-2, CIN-3, and CIN-4. GABA identification of all the LAB isolates using TLC Silica Gel 60 F254 with butanol: acetic acid: distilled water (5: 3: 2) as eluent yielded Rf 0.61 and Rf MSG 0.38. The highest growth was achieved by isolate CIN-3 with an absorbance of 1.488 (at 48 hour) in non-MSG medium, while the addition of 5% MSG resulted in an absorbance of 1.631 (at 42 hour). GABA production was achieved by isolate CIN-3 with 5% MSG treatment with a concentration of 201.472 mM and without MSG with a concentration of 171.195 mM. Cincalok merupakan pangan fermentasi yang berasal dari Kalimantan Barat. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat (BAL) yang mampu menghasilkan gamma-aminobutyric acid (GABA), melakukan karakterisasi isolat BAL yang diperoleh dan dapat diperoleh GABA dengan metode Kromatografi Lapis Tipis (KLT). Penumbuhan bakteri dan produksi GABA dilakukan dengan penambahan MSG 5% dan tanpa MSG, dan diukur menggunakan spektrofotometer. Dalam penelitian ini diperoleh 4 isolat bakteri BAL yang diberi kode CIN-1, CIN-2, CIN-3, dan CIN-4. Identifikasi GABA dari semua isolat BAL tersebut menggunakan KLT Silica Gel 60 F254 dengan eluen butanol: asam asetat: aquades (5: 3: 2), menghasilkan Rf 0,61 dan Rf MSG 0,38. Pertumbuhan tertinggi terjadi pada isolat CIN-3 non MSG dengan absorbansi 1,488 (jam ke-48), sedangkan dengan penambahan MSG 5% menghasilkan absorbansi 1,631 (jam ke-42). Produksi GABA dicapai isolat CIN-3 dengan perlakuan MSG 5% dengan konsentrasi 201.472 mM dan tanpa MSG dengan konsentrasi 171,195 mM.

scholarly journals Complete Genome Sequence of Streptococcus thermophilus Strain B59671, Which Naturally Produces the Broad-Spectrum Bacteriocin Thermophilin 110

2017 ◽  
Vol 5 (45) ◽  
Author(s):  
John A. Renye ◽  
David S. Needleman ◽  
George A. Somkuti ◽  
Dennis H. Steinberg
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Genome Sequence ◽  
Broad Spectrum ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Streptococcus Thermophilus ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Content Type

ABSTRACT Streptococcus thermophilus strain B59671 is a Gram-positive lactic acid bacterium that naturally produces a broad-spectrum bacteriocin, thermophilin 110, and is capable of producing gamma-aminobutyric acid (GABA). The complete genome sequence for this strain contains 1,821,173 nucleotides, 1,936 predicted genes, and an average G+C content of 39.1%.

scholarly journals Production of Gamma-Aminobutyric Acid from Lactic Acid Bacteria: A Systematic Review

2020 ◽  
Vol 21 (3) ◽  
pp. 995 ◽  
Author(s):  
Yanhua Cui ◽  
Kai Miao ◽  
Siripitakyotin Niyaphorn ◽  
Xiaojun Qu
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Genetic Engineering ◽  
Molecular Mechanisms ◽  
Aminobutyric Acid ◽  
Research Progress ◽  
Gamma Aminobutyric Acid ◽  
Culture Methods ◽  
Gaba Production ◽  
Gaba Biosynthesis

Gamma-aminobutyric acid (GABA) is widely distributed in nature and considered a potent bioactive compound with numerous and important physiological functions, such as anti-hypertensive and antidepressant activities. There is an ever-growing demand for GABA production in recent years. Lactic acid bacteria (LAB) are one of the most important GABA producers because of their food-grade nature and potential of producing GABA-rich functional foods directly. In this paper, the GABA-producing LAB species, the biosynthesis pathway of GABA by LAB, and the research progress of glutamate decarboxylase (GAD), the key enzyme of GABA biosynthesis, were reviewed. Furthermore, GABA production enhancement strategies are reviewed, from optimization of culture conditions and genetic engineering to physiology-oriented engineering approaches and co-culture methods. The advances in both the molecular mechanisms of GABA biosynthesis and the technologies of synthetic biology and genetic engineering will promote GABA production of LAB to meet people’s demand for GABA. The aim of the review is to provide an insight of microbial engineering for improved production of GABA by LAB in the future.

scholarly journals Construction of a Vitreoscilla Hemoglobin Promoter-Based Tunable Expression System for Corynebacterium glutamicum

Catalysts ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 561 ◽  
Author(s):  
Kei-Anne Baritugo ◽  
Hee Taek Kim ◽  
Mi Na Rhie ◽  
Seo Young Jo ◽  
Tae Uk Khang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Fluorescent Protein ◽  
Expression System ◽  
Vitreoscilla Hemoglobin ◽  
Aminobutyric Acid ◽  
Vector System ◽  
Efficient Production ◽  
Gamma Aminobutyric Acid ◽  
Recombinant Strains ◽  
Gaba Production

Corynebacterium glutamicum is an industrial strain used for the production of valuable chemicals such as L-lysine and L-glutamate. Although C. glutamicum has various industrial applications, a limited number of tunable systems are available to engineer it for efficient production of platform chemicals. Therefore, in this study, we developed a novel tunable promoter system based on repeats of the Vitreoscilla hemoglobin promoter (Pvgb). Tunable expression of green fluorescent protein (GFP) was investigated under one, four, and eight repeats of Pvgb (Pvgb, Pvgb4, and Pvgb8). The intensity of fluorescence in recombinant C. glutamicum strains increased as the number of Pvgb increased from single to eight (Pvgb8) repeats. Furthermore, we demonstrated the application of the new Pvgb promoter-based vector system as a platform for metabolic engineering of C. glutamicum by investigating 5-aminovaleric acid (5-AVA) and gamma-aminobutyric acid (GABA) production in several C. glutamicum strains. The profile of 5-AVA and GABA production by the recombinant strains were evaluated to investigate the tunable expression of key enzymes such as DavBA and GadBmut. We observed that 5-AVA and GABA production by the recombinant strains increased as the number of Pvgb used for the expression of key proteins increased. The recombinant C. glutamicum strain expressing DavBA could produce higher amounts of 5-AVA under the control of Pvgb8 (3.69 ± 0.07 g/L) than the one under the control of Pvgb (3.43 ± 0.10 g/L). The average gamma-aminobutyric acid production also increased in all the tested strains as the number of Pvgb used for GadBmut expression increased from single (4.81–5.31 g/L) to eight repeats (4.94–5.58 g/L).

scholarly journals Characterization of γ-Aminobutyric acid(GABA) produced by a lactic acid bacterium from button mushroom bed

2013 ◽  
Vol 11 (4) ◽  
pp. 181-186 ◽  
Author(s):  
Yun-Seok Lee ◽  
Tae-Young Song ◽  
Won-Sik Kong ◽  
Min-Ho Yoon
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacterium ◽  
Aminobutyric Acid ◽  
Button Mushroom

153 Sperm metabolomic landscape associated with bull fertility

2019 ◽  
Vol 31 (1) ◽  
pp. 201
Author(s):  
E. Menezes ◽  
F. Santos ◽  
A. Velho ◽  
T. Dinh ◽  
A. Kaya ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Lactic Acid ◽  
Oleic Acid ◽  
Benzoic Acid ◽  
Organic Acids ◽  
Low Fertility ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
General Decline ◽  
Bull Sperm

Sub-fertility fertility in bulls decreases the efficiency and profitability of cattle production because AI allows a single bull to inseminate thousands of cows. In recent decades, there has been a general decline in fertility of bulls, even among those exhibiting normal sperm motility and morphology. Despite advances in technology and knowledge, molecular, cellular and physiological mechanisms underlying the causes of low fertility in bulls are currently unclear. Therefore, the objective of this study was to identify sperm metabolites associated with fertility in Holstein bulls. The metabolome of sperm from 10 mature bulls with high fertility (HF, n=5) and low fertility (LF, n=5) was identified by gas chromatography coupled to mass spectrometry. Bull fertility was based on the sire conception rates deviating from the average. Statistical analysis was performed by using MetaboAnalyst 3.0 (http://www.metaboanalyst.ca/). A total of 22 metabolites were detected and categorized according to their major chemical classes, including amino acids, peptides/analogues, carbohydrates/carbohydrate conjugates, fatty acids, steroids/steroid derivatives, keto acids and derivatives, carboxylic acids, and other organic and inorganic compounds. Organic acids and derivatives as well as fatty acids were the major compounds in bull spermatozoa. Seven organic acids and derivatives were detected, including benzoic acid, carbonate, carbamate dimethyl, carbamate trimethyl, lactic acid, oxalic acid, and urea. Five fatty acids were identified including oleic acid, oleanitrile, nonanoic acid, and palmitic acid. Oleic acid, phosphoric acid, phosphine, carbamate trimethyl, and glycerol were the most abundant metabolites in bull sperm, whereas benzoic acid, acetic acid, l-serine, carbamate, and 2-ketobutyric acid were the least predominant metabolites present in bull sperm. Multivariate analysis (partial least squares-discriminant analysis) of the sperm metabolome showed a clear separation between HF and LF bulls. Variable importance in projection (VIP) score demonstrated that metabolites with VIP >1.5 were gamma-aminobutyric acid (VIP=2.01), carbamate trimethyl (VIP=1.88), benzoic acid (VIP=1.86), and lactic acid (VIP=1.81). Abundance ratios of gamma-aminobutyric acid, carbamate trimethyl, benzoic acid, and lactic acid was greater in HF bulls compared with LF animals. According to univariate analysis, abundance ratios of gamma-aminobutyric acid (P=0.03) and carbamate trimethyl (P=0.047) were greater in HF than in LF bulls. Gamma-aminobutyric acid was positively correlated with carbamate trimethyl (r=0.94; P<0.0001) and benzoic acid (r=0.74; P=0.0139). Benzoic acid was positively correlated with carbamate trimethyl (r=0.75; P=0.0107) and carbamate dimethyl (r=0.68; P=0.0274). The identified metabolites can serve as potential markers to evaluate semen quality and predict bull fertility.

Sign in / Sign up

Export Citation Format

Share Document