Enzyme-assisted extraction of carbohydrates from the brown alga Ecklonia radiata : Effect of enzyme type, pH and buffer on sugar yield and molecular weight profiles

2016 ◽  
Vol 51 (10) ◽  
pp. 1503-1510 ◽  
Author(s):  
Suvimol Charoensiddhi ◽  
Andrew J. Lorbeer ◽  
Jelle Lahnstein ◽  
Vincent Bulone ◽  
Christopher M.M. Franco ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Brown Alga ◽  
Sugar Yield ◽  
Ecklonia Radiata ◽  
Assisted Extraction ◽  
Enzyme Assisted Extraction

scholarly journals Enzyme-Assisted Fucoidan Extraction from Brown Macroalgae Fucus distichus subsp. evanescens and Saccharina latissima

Marine Drugs ◽  
2020 ◽  
Vol 18 (6) ◽  
pp. 296 ◽  
Author(s):  
Thuan Thi Nguyen ◽  
Maria Dalgaard Mikkelsen ◽  
Vy Ha Nguyen Tran ◽  
Vo Thi Dieu Trang ◽  
Nanna Rhein-Knudsen ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Alginate Lyase ◽  
Saccharina Latissima ◽  
Brown Macroalgae ◽  
Brown Seaweeds ◽  
Molecular Weights ◽  
Fucus Distichus ◽  
Assisted Extraction ◽  
High Yields ◽  
Enzyme Assisted Extraction

Fucoidans from brown macroalgae (brown seaweeds) have different structures and many interesting bioactivities. Fucoidans are classically extracted from brown seaweeds by hot acidic extraction. Here, we report a new targeted enzyme-assisted methodology for fucoidan extraction from brown seaweeds. This enzyme-assisted extraction protocol involves a one-step combined use of a commercial cellulase preparation (Cellic®CTec2) and an alginate lyase from Sphingomonas sp. (SALy), reaction at pH 6.0, 40 °C, removal of non-fucoidan polysaccharides by Ca2+ precipitation, and ethanol-precipitation of crude fucoidan. The workability of this method is demonstrated for fucoidan extraction from Fucus distichus subsp. evanescens (basionym Fucus evanescens) and Saccharina latissima as compared with mild acidic extraction. The crude fucoidans resulting directly from the enzyme-assisted method contained considerable amounts of low molecular weight alginate, but this residual alginate was effectively removed by an additional ion-exchange chromatographic step to yield pure fucoidans (as confirmed by 1H NMR). The fucoidan yields that were obtained by the enzymatic method were comparable to the chemically extracted yields for both F. evanescens and S. latissima, but the molecular sizes of the fucoidans were significantly larger with enzyme-assisted extraction. The molecular weight distribution of the fucoidan fractions was 400 to 800 kDa for F. evanescens and 300 to 800 kDa for S. latissima, whereas the molecular weights of the corresponding chemically extracted fucoidans from these seaweeds were 10–100 kDa and 50–100 kDa, respectively. Enzyme-assisted extraction represents a new gentle strategy for fucoidan extraction and it provides new opportunities for obtaining high yields of native fucoidan structures from brown macroalgae.

Enzyme-Assisted Extraction of Moniliformin from Extruded Corn Grits

2005 ◽  
Vol 53 (13) ◽  
pp. 5074-5078 ◽  
Author(s):  
Soo-Hyun Chung ◽  
Dojin Ryu ◽  
Eun-Kyung Kim ◽  
Lloyd B. Bullerman
Keyword(s):  
Assisted Extraction ◽  
Enzyme Assisted Extraction

Recovery of Biomolecules from Agroindustry by Solid-Liquid Enzyme-Assisted Extraction: a Review

2021 ◽  
Author(s):  
Elisa Hernández Becerra ◽  
Eduardo De Jesús Pérez López ◽  
Jhon Wilder Zartha Sossa
Keyword(s):  
Assisted Extraction ◽  
Solid Liquid ◽  
Enzyme Assisted Extraction

Detection of aflatoxin M1in milk, cheese and sour cream samples from Costa Rica using enzyme-assisted extraction and HPLC

2015 ◽  
Vol 8 (2) ◽  
pp. 128-135 ◽  
Author(s):  
Guadalupe Chavarría ◽  
Fabio Granados-Chinchilla ◽  
Margarita Alfaro-Cascante ◽  
Andrea Molina
Keyword(s):  
Costa Rica ◽  
Assisted Extraction ◽  
Sour Cream ◽  
Enzyme Assisted Extraction

Process and economic feasibility for the production of functional food from the brown alga Ecklonia radiata

2018 ◽  
Vol 29 ◽  
pp. 80-91 ◽  
Author(s):  
Suvimol Charoensiddhi ◽  
Andrew J. Lorbeer ◽  
Christopher M.M. Franco ◽  
Peng Su ◽  
Michael A. Conlon ◽  
...  
Keyword(s):  
Functional Food ◽  
Brown Alga ◽  
Economic Feasibility ◽  
Ecklonia Radiata
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