scholarly journals Co-immobilization and stabilization of xylanase, β-xylosidase and α-l-arabinofuranosidase from Penicillium janczewskii for arabinoxylan hydrolysis

2016 ◽  
Vol 51 (5) ◽  
pp. 614-623 ◽  
Author(s):  
César Rafael Fanchini Terrasan ◽  
Lara Trobo-Maseda ◽  
Sonia Moreno-Pérez ◽  
Eleonora Cano Carmona ◽  
Benevides Costa Pessela ◽  
...  
Keyword(s):  
Penicillium Janczewskii

Cloning and functional expression of α-galactosidase cDNA from Penicillium janczewskii zaleski

Biologia ◽  
2011 ◽  
Vol 66 (2) ◽  
Author(s):  
Bo Zhang ◽  
Yiqun Chen ◽  
Zhimin Li ◽  
Wenqing Lu ◽  
Yunhe Cao
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Functional Expression ◽  
Recombinant Enzyme ◽  
Methanol Induction ◽  
Potential Applications ◽  
Prospective Application ◽  
Polymerase Chain ◽  
Penicillium Janczewskii ◽  
Rapid Amplification ◽  
Different Levels

AbstractIn recent years, α-galactosidase has been attracting more and more attention because of its potential applications in many aspects. Using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends, a full-length cDNA sequence composed of 2,439 bp was cloned from Penicillium janczewskii zaleski and was subcloned into pPICZαA and transformed into Pichia pastoris strain X-33. In a 10-L fermentor, the recombinant yeast expressed α-galactosidase with a yield of 254 U/mL by methanol induction for 120 h. The recombinant enzyme showed the optimal activity at 40°C and pH 5.2. The K m values of the recombinant enzyme using p-nitrophenyl-α-D-galactopyranoside (pNPG), melibiose, raffinose and stachyose as substrates were 1, 16, 17.8 and 5.3 mM, respectively. V max values were 227.3, 116.7, 104.8, and 80.6 μM/min using pNPG, melibiose, raffinose and stachyose as substrates, respectively. The α-galactosidase exhibited no sensitivity to various metal ions and ethylenediaminetetraacetic acid, and hydrolyzed melibiose, raffinose and stachyose with different levels of galactose release. The biochemical characteristics of the α-galactosidase suggest that the enzyme may have a prospective application in feed industry as an additive.

scholarly journals Produção de concentrados de frutose por inulinases de Penicillium janczewskii e atividade sobre o nível de glicose plasmática em ratos diabéticos

2004 ◽  
Vol 24 (3) ◽  
pp. 373-377 ◽  
Author(s):  
Rosemeire A. Bom Pessoni ◽  
Pedro M. O. Olmedo ◽  
Ana C. Clemente Filha ◽  
Rita C. L. Figueiredo-Ribeiro
Keyword(s):  
Penicillium Janczewskii

scholarly journals Immobilization and Stabilization of Beta-Xylosidases from Penicillium janczewskii

2016 ◽  
Vol 182 (1) ◽  
pp. 349-366 ◽  
Author(s):  
César Rafael Fanchini Terrasan ◽  
Maria Romero-Fernández ◽  
Alejandro H. Orrego ◽  
Sandro Martins Oliveira ◽  
Benevides Costa Pessela ◽  
...  
Keyword(s):  
Penicillium Janczewskii

scholarly journals Woronin bodies in Penicillium janczewskii Zaleski

2002 ◽  
Vol 33 (2) ◽  
Author(s):  
Rosemeire A.B. Pessoni ◽  
Glenn Freshour ◽  
Rita de Cássia L. Figueiredo-Ribeiro ◽  
Michael G. Hahn ◽  
Márcia R. Braga
Keyword(s):  
Penicillium Janczewskii

scholarly journals CHARACTERISTICS OF MICROBIOLOGICAL AND AGROCHEMICAL COMPOSITION OF EFLUENT ORGANIC FERTILIZER

2019 ◽  
pp. 45-55
Author(s):  
Vitalii Palamarchuk ◽  
Vadym Krychkovskyi
Keyword(s):  
Biogas Production ◽  
Organic Fertilizer ◽  
Pathogenic Fungi ◽  
Organic Fertilizers ◽  
Pig Manure ◽  
Microbiological Analysis ◽  
Pathogenic Species ◽  
Saprotrophic Fungi ◽  
Alkaline Reaction ◽  
Penicillium Janczewskii

The article presents the results of Efluent organic fertilizer research. We used pig manure to obtain biomass (residues); it also provided biogas production after digestion. We used a biogas system located in LLC Organic-D for fermentation of biomass, it is located in the village of Sutysky, Tyvriv district, Vinnytsia region, Ukraine. The prospects of using similar types of fertilizers have been analyzed. Agrochemical and microbiological analysis was carried out in specialized laboratories for organic fertilizers. It has been established that the peculiarities of animal maintenance and feeding have affected the chemical composition of manure, it is confirmed by the data of microbiological and agrochemical analysis, in particular the level of its invasive contamination. The total number of fungi in the samples ranged from 118.8 to 193.8 thousand / g. The species composition of pathogenic fungi in manure were pathogenic species of the genus Alternaria and Fusarium by 3.2%, and the number of pathogenic species of fungi not rejuvenated manure was 66.7% (from the genus Fusarium - 9.5%, from the genus Aspergillus - 57.2%). Among saprotrophic fungi there are species from the genus Penicillium (Penicillium janczewskii Zaleski, P. raciborskii Zaleski, P. simplicissimum (Oudem.) Thom, P. chryzogenum Thom) from the genus Acremonium (Acremonium Kiliense Grutz). In the first sample (fermented) the number of saprotrophic fungi from the genus Penicillium was 87.1%, from the genus Acremonium 6.5%. In the second sample (without fermentation), the number of saprotrophic fungi of the genus Penicillium decreased to 33.3%. Saprotrophic fungi of the genus Acremonium were not observed. Among toxin-forming species, Penicillium janczewskii Zaleski, Penicillium chryzogenum Thom, Aspergillus flavus, Alternaria alternata (Fr.) Keissl. and Fusarium oxysporum (Schlecht.) Snyd. et Hans) were observed. A high amount of toxin-forming fungi was found in undiluted manure - 85.7%. In the updated material, the percentage of toxin-forming fungi was lower by 16.1%. Organic fertilizer Efluent was characterized by an alkaline reaction (pH 8.5), high moisture content (98.4%), significant content of nitrate nitrogen (18.2 mg/kg), copper (4.6 mg/kg), zinc (32 mg/kg), manganese (20 mg/kg) and iron (120 mg/kg). Therefore, the use of this fertilizer will provide plants with both macro and microelements, and its application on acidic soils will also reduce the level of soil acidity. Prospects of its use in soils with the raised acidity of a soil solution are given.

Directed Evolution of Penicillium janczewskii zalesk α-Galactosidase Toward Enhanced Activity and Expression in Pichia pastoris

2012 ◽  
Vol 168 (3) ◽  
pp. 638-650 ◽  
Author(s):  
Yiqun Chen ◽  
Bo Zhang ◽  
Honglei Pei ◽  
Junnan Lv ◽  
Wenhan Yang ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Directed Evolution ◽  
Enhanced Activity ◽  
Penicillium Janczewskii
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