Comparative study of sulfur utilization and speciation transformation of two elemental sulfur species by thermoacidophilic Archaea Acidianus manzaensis YN-25

2013 ◽  
Vol 48 (12) ◽  
pp. 1855-1860 ◽  
Author(s):  
Hong-Chang Liu ◽  
Jin-Lan Xia ◽  
Zhen-Yuan Nie ◽  
An-An Peng ◽  
Chen-Yan Ma ◽  
...  
Keyword(s):  
Comparative Study ◽  
Elemental Sulfur ◽  
Sulfur Species ◽  
Thermoacidophilic Archaea ◽  
Acidianus Manzaensis

Comparative Study of Iron and Sulfur Speciation Transformation of Pyrite and Marcasite by Extreme Thermophilic Archaea Acidianus manzaensis

2015 ◽  
Vol 1130 ◽  
pp. 342-346
Author(s):  
Hong Chang Liu ◽  
Jin Lan Xia ◽  
Zhen Yuan Nie ◽  
Hong Rui Zhu ◽  
Yi Dong Zhao ◽  
...  
Keyword(s):  
Elemental Sulfur ◽  
Sulfur Species ◽  
Edge Structure ◽  
X Ray ◽  
Mineral Structure ◽  
Sulfur Speciation ◽  
Acidianus Manzaensis ◽  
X Ray Absorption ◽  
Lower Oxidation ◽  
Thermophilic Archaea

Differential utilization of isomers pyrite and marcasite by Acidianus manzaensis were comparatively studied, besides the iron and sulfur speciation transformation of the minerals was also investigated based on synchrotron radiation X-ray absorption near edge structure (XANES) spectroscopy. The results showed that the biooxidation of pyrite was faster than marcasite. The bioleached surface of both pyrite and marcasite are serious corroded, and the Fe (III)-containing species as well as jarosite were gradually produced, and more elemental sulfur species were formed on the marcasite surface than that for pyrite. It demonstrates the mineral structure does affect the biooxidation of pyrite and marcasite, and the more precipitated elemental sulfur might be one of the reasons leading to lower oxidation rate of marcasite by A. manzaensis.

Identification of Sulfur Activation Relevant Protein Genes of Extremely Thermophilic Acidianus manzaensis

2017 ◽  
Vol 262 ◽  
pp. 461-465 ◽  
Author(s):  
Hong Chang Liu ◽  
Jin Lan Xia ◽  
Zhen Yuan Nie ◽  
Ya Long Ma ◽  
Yun Yang ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Elemental Sulfur ◽  
Extracellular Proteins ◽  
Laser Desorption Ionization ◽  
Two Dimensional Gel Electrophoresis ◽  
Ionization Time ◽  
Real Time Quantitative Pcr ◽  
Flight Mass Spectrometry ◽  
Genes Encoding ◽  
Acidianus Manzaensis

The sulfur activation by extracellular proteins is considered as the crucial stage during biooxidation of elemental sulfur (S0). In order to study genes encoding sulfur-activation related extracellular proteins of extremely thermophilic Acidianus manzaensis, the extracellular proteins with higher abundance for the strain grown on S0 allotropes than that on Fe2+ were first screened by two-dimensional gel electrophoresis, and then identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Nine genes amplified with PCR were satisfactory according to their agarose gel electrophoresis. The differential expression of these nine genes when the strain grown on S0 allotropes and Fe2+ were analyzed with real-time quantitative PCR (RT-qPCR). Results showed that seven of them were higher expressed when the strain grown on S0 allotropes than on Fe2+, indicating they may be related with sulfur activation by A. manzaensis.

Evolution of Compositions and Contents of Capsule and Slime EPSs for Adaptation to and Action on Energy Substrates and Heavy Metals by Typical Bioleaching Microorganisms

2017 ◽  
Vol 262 ◽  
pp. 466-470 ◽  
Author(s):  
Zhen Yuan Nie ◽  
Hong Chang Liu ◽  
Jin Lan Xia ◽  
Huan Liu ◽  
Yun Lu Cui ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal Ions ◽  
Elemental Sulfur ◽  
Uronic Acids ◽  
Energy Substrates ◽  
Inorganic Matter ◽  
Acidianus Manzaensis ◽  
First Time ◽  
Extracellular Polymer ◽  
Mineral Interaction

Adaption to the energy substrates and heavy metals by bioleaching micoorganisms is the prerequisite for efficient microbe-mineral interaction in bioleaching process. It is known extracellular polymer substances (EPSs) take important role in mediating the adaption to and action on energy substrates and heavy metals. This report presents the evolution of compositions and contents of the major components of EPSs of the typical bioleaching microorganisms (Acidithiobacillus ferrooxidans, Leptospirillum ferriphilum, Sulfobacillus thermosulfidooxidans, and Acidianus manzaensis,) exposed to different energy substrates and heavy metal ions. These strains were acclimated firstly to Fe2+ substrate, and then on the substrates elemental sulfur (S0), pyrite and chalcopyrite, respectively. It was found that the major components of capsule and slime EPSs in terms of proteins, polysaccharides, as well as uronic acids were quite different in contents for the Fe2+-acclimated strains, and they even changed more when the Fe2+-acclimated strains were further acclimated to the other substrates. When exposed to heavy metals, all strains demonstrated much decrease in contents of capsule EPSs, and much increase in slime EPSs contents and the heavy metals were found to bound to the slime parts. It was for the first time the EPSs of the bioleaching strains were fractionated into capsule part and slime part, and it was also for the first time we found the differences in evolution of compositions and contents of the major organic components as well as the inorganic matter of capsule EPSs and slime EPSs when the bioleaching strains were exposed to different energy substrates and heavy metals.

scholarly journals Insight Into Interactions of Thermoacidophilic Archaea With Elemental Sulfur: Biofilm Dynamics and EPS Analysis

2019 ◽  
Vol 10 ◽  
Author(s):  
Ruiyong Zhang ◽  
Thomas R. Neu ◽  
Qian Li ◽  
Véronique Blanchard ◽  
Yutong Zhang ◽  
...  
Keyword(s):  
Elemental Sulfur ◽  
Thermoacidophilic Archaea ◽  
Insight Into

Bioleaching of Enargite by Arsenic-Tolerant Acidithiobacillus Ferrooxidans

2009 ◽  
Vol 71-73 ◽  
pp. 485-488 ◽  
Author(s):  
Keiko Sasaki ◽  
Koichiro Takatsugi ◽  
Tsuyoshi Hirajima ◽  
Naofumi Kozai ◽  
Toshihiko Ohnuki ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Infrared Spectroscopy ◽  
Photoelectron Spectroscopy ◽  
Acidithiobacillus Ferrooxidans ◽  
Elemental Sulfur ◽  
Oxidative Dissolution ◽  
X Ray Diffraction ◽  
Sulfur Species ◽  
Secondary Minerals ◽  
X Ray

Acidithiobacillus ferrooxidans was acclimatized to 1000 mg/L arsenic(V) and then used for the bioleaching of enargite (Cu3AsS4) at pH 2. Secondary minerals formed during the bioleaching of enargite were characterized by X-ray diffraction, FT- infrared spectroscopy, Raman spectroscopy and X-ray photoelectron spectroscopy. Oxidative dissolution of enargite resulted in the formation of elemental sulfur, arsenate and oxidized sulfur species including jarosites and possibly also schwertmannite at pH 2.

scholarly journals Complete Genome Sequence of the Extremely Thermoacidophilic Archaeon Acidianus manzaensis YN-25

2017 ◽  
Vol 5 (25) ◽  
Author(s):  
Ya-long Ma ◽  
Jin-lan Xia ◽  
Yun Yang ◽  
Zhen-yuan Nie ◽  
Hong-chang Liu ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Dna Sequences ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Elemental Sulfur ◽  
Sulfur Compounds ◽  
Inorganic Sulfur ◽  
Acidianus Manzaensis

ABSTRACT The complete genome of Acidianus manzaensis YN-25 consists of a chromosome of 2,687,463 bp, with a G+C content of 30.62% and 2,746 coding DNA sequences. This archaeon contains a series of specific genes involved in the oxidation of elemental sulfur and reduced inorganic sulfur compounds.

Heavy metal ions removed from imitating acid mine drainages with a thermoacidophilic archaea: Acidianus manzaensis YN25

2020 ◽  
Vol 190 ◽  
pp. 110084 ◽  
Author(s):  
Mengke Li ◽  
Yongji Huang ◽  
Yanping Yang ◽  
Haibei Wang ◽  
Liang Hu ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Acid Mine ◽  
Thermoacidophilic Archaea ◽  
Acidianus Manzaensis
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