Direct conversion of inulin into single cell protein by the engineered Yarrowia lipolytica carrying inulinase gene

2011 ◽  
Vol 46 (7) ◽  
pp. 1442-1448 ◽  
Author(s):  
Wei Cui ◽  
Qi Wang ◽  
Fang Zhang ◽  
Shi-Cui Zhang ◽  
Zhen-Ming Chi ◽  
...  
Keyword(s):  
Single Cell ◽  
Yarrowia Lipolytica ◽  
Direct Conversion ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Inulinase Gene

scholarly journals Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica

2007 ◽  
Vol 10 (3) ◽  
pp. 0-0 ◽  
Author(s):  
Seraphim Papanikolaou ◽  
Isabelle Chevalot ◽  
Maria Galiotou-Panayotou ◽  
Michael Komaitis ◽  
Ivan Marc ◽  
...  
Keyword(s):  
Single Cell ◽  
Yarrowia Lipolytica ◽  
Lipase Production ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Microbial Lipid

scholarly journals Effect of Plant Oils upon Lipase and Citric Acid Production inYarrowia lipolyticaYeast

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Farshad Darvishi ◽  
Iraj Nahvi ◽  
Hamid Zarkesh-Esfahani ◽  
Fariborz Momenbeik
Keyword(s):  
Citric Acid ◽  
Olive Oil ◽  
Single Cell ◽  
Yarrowia Lipolytica ◽  
Organic Nitrogen ◽  
Low Cost ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Plant Oils ◽  
Single Cell Oil

The nonconventional yeastYarrowia lipolyticadegrades very efficiently hydrophobic substrates to produce organic acids, single-cell oil, lipases, and so forth. The aim of this study was to investigate the biochemical behavior and simultaneous production of valuable metabolites such as lipase, citric acid (CA), and single-cell protein (SCP) byYarrowia lipolyticaDSM 3286 grown on various plant oils as sole carbon source. Among tested plant oils, olive oil proved to be the best medium for lipase and CA production. TheY. lipolyticaDSM 3286 produced 34.6 0.1 U/mL of lipase and also CA and SCP as by-product on olive oil medium supplemented with yeast extract. Urea, as organic nitrogen, was the best nitrogen source for CA production. The results of this study suggest that the two biotechnologically valuable products, lipase and CA, could be produced simultaneously by this strain using renewable low-cost substrates such as plant oils in one procedure.

scholarly journals Waste Cooking Oil as Substrate for Single Cell Protein Production by Yeast Yarrowia lipolytica

2020 ◽  
Vol 24 (3) ◽  
pp. 457-469
Author(s):  
Kriss Spalvins ◽  
Zane Geiba ◽  
Zane Kusnere ◽  
Dagnija Blumberga
Keyword(s):  
Carbon Source ◽  
Single Cell ◽  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Waste Cooking Oil ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Cooking Oil ◽  
Cooking Oils ◽  
Single Cell Protein Production

AbstractCooking oils are widely used in food preparation. During cooking, harmful compounds are formed in oils, therefore utilization of used cooking oils (waste cooking oils) is limited. Single cell protein (SCP) is dietary protein, which can be produced from various protein-rich microorganisms that are capable of utilizing industrial by-products such as waste cooking oil (WCO). In this study the utilization of industrial WCO (obtained from local potato chips manufacturer) as a carbon source for single cell protein production by yeast Yarrowia lipolytica was assessed. The medium containing 27.5 g/L WCO and C/N ratio of 5–10 for batch fermentations was determined to be the optimal composition for SCP production. In this study, the highest reported Yarrowia lipolytica biomass concentration (57.37 g/L) was achieved when WCO was used as the main carbon source. Protein concentrations were relatively low (12.6 %), which also affected the final protein yield (7.23 g/L). The resulting biomass accumulated low concentrations of toxic malondialdehyde (MDA) (2.32 mg MDA/kg) compared to concentrations initially detected in the WCO itself (30.87 mg MDA/kg). To the best of the authors knowledge this is the first study to report on MDA decrease via microbial fermentations.

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